Alpha factor mating pheromone Yeast

GenWay ID: GWB-E29545
Legacy ID: 06-271-83056
Size: 10 mg
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Details for Alpha factor mating pheromone Yeast

Sequence (One Letter Code) WHWLQLKPGQPMY
Sequence {TRP} {HIS} {TRP} {LEU} {GLN} {LEU} {LYS} {PRO} {GLY} {GLN} {PRO} {MET} {TYR}
MW 1683.98.
CAS 59401-28-4.
Formula C82H114N20O17S.
Solubility Soluble in water. Thaw and use. Recommended concentrations raging from 5uM (1bar D) to 100uM (BAR1).
Notes Simply thaw and use directly. We recommend using it at concentrations ranging from 5 uM (bar1 D) to 100 uM (BAR1). The alpha factor pheromone arrests yeast in G1 of the cell cycle. Alpha factor mating pheromone induces the expression of mating genes. Alpha factor mating pheromone changes in nuclear architecture. and Alpha factor mating pheromone polarized growth toward the mating partner. STE2 encodes the alpha factor pheromone receptor (a GPCR) found on mating-type a cells in the yeast.
Function The active factor is excreted into the culture medium by haploid cells of the A mating type and acts on cells of the opposite mating type (type alpha). It mediates the conjugation process between the two types by inhibiting the initiation of DNA synthesis in type alpha cells and synchronizing them with type A.
Subcellular Location Cell membrane; lipid-anchor; cytoplasmic side (Potential).
Miscellaneous Present with 28900 molecules/cell.
References
  1. Brake,A.J., Brenner,C., Najarian,R., Laybourn,P. and Merryweather,J.
    Structure of genes encoding precursors of the yeast peptide mating pheromone a-factor
  2. Michaelis,S. and Herskowitz,I., et al.
    The a-factor pheromone of Saccharomyces cerevisiae is essential for mating
  3. Jacq,C., Alt-Moerbe,J., Andre,B., Arnold,W., Bahr,A., Ballesta,J.P.G., Bargues,M., Baron,L., Becker,A., Biteau,N.,, et al.
    The nucleotide sequence of Saccharomyces cerevisiae chromosome IV
  4. Marsischky,G., Rolfs,A., Richardson,A., Kane,M., Baqui,M., Taycher,E., Hu,Y., Vannberg,F., Weger,J., Kramer,J., Moreira,D.,, et al.
    Direct Submission
  5. Betz,R., Crabb,J.W., Meyer,H.E., Wittig,R. and Duntze,W., et al.
    Amino acid sequences of a-factor mating peptides from Saccharomyces cerevisiae
  6. Brake A.J., Brenner C., Najarian R., Laybourn P., Merryweather J.
    Structure of genes encoding precursors of the yeast peptide mating pheromone a-factor.
  7. Michaelis S., Herskowitz I.
    The a-factor pheromone of Saccharomyces cerevisiae is essential for mating.
  8. Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., et al.
    The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
  9. Marsischky G., Rolfs A., Richardson A., Kane M., Baqui M., Taycher E., Hu Y., Vannberg F., Weger J., Kramer J., et al.
    Creation of the YFLEX clone resource: cloning of Saccharomyces cerevisiae ORFs in the Gateway recombinational cloning system.
  10. Betz R., Crabb J.W., Meyer H.E., Wittig R., Duntze W.
    Amino acid sequences of a-factor mating peptides from Saccharomyces cerevisiae..

Additional Info for Alpha factor mating pheromone Yeast

Name Alpha factor mating pheromone Yeast
Related Product Names Alpha factor mating pheromone YeastMFA1
NCBI Acc Number P34165
GI Number 462595
Purity > 95%
Storage Store this peptide in its dry form at 4C. For best results, rehydrate the peptide immediately before use. Do not re-freeze any unused portions.
Molecular Weight 1684.2
Sequence Length 36
Swiss Prot Number P34165

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