Details for BMP-2 Human
Amino Acid Sequence: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC CPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR
Biological Activity: 1. The ED50 as determined by its ability to induce alkaline phosphatase production by ATDC-5 cells is 0.5-1.0 ug/ml.
Protein Content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of BMP-2 as a Reference Standard.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility: It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-2 in sterile 100mM AcOH (acetic Acid) not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Bone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton.
The BMP-2 is purified by proprietary chromatographic techniques
BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia (myositis) ossificans progressiva.
Function: Induces cartilage and bone formation.
Subunit: Homodimer; disulfide-linked. Interacts with GREM2 (By similarity) and SOSTDC1.
Subcellular Location: Secreted.
Tissue Specificity: Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.
Similarity: Belongs to the TGF-beta family.
Additional Info for BMP-2 Human
|Related Product Names||BMP-2, BMP2A.; BMP-2; BMP-2A BMP2ABMP-2 HumanBMP2|
|NCBI Acc Number||NP_001191.1|
|Purity||Greater than 95.0% as determined by:(a) Analysis by RP-HPLC
(b) Analysis by SDS-PAGE.
|Format||BMP-2 was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5.|
|Stability||Lyophilized Bone Morphogenetic Protein-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution BMP2 should be stored at 4 C between 2-7 days and for future use below -18 C.
For long term storage,
|Swiss Prot Number||P12643|