Specificity: CD134

NCBI Gene ID: 22163

Isotype: Specificity: CD134

Clone: OX-86 Immunogen: CHO cells transfected with murine OX40

Fusion Partner: Spleen cells from immunised AO rats were fused with cells of the mouse NS1 myeloma cell line.

Specificity Note: This product recognises murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes. Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution: Phosphate buffered saline pH7.4

Preservative Stabilisers: 0.09%Sodium Azide1%Bovine Serum Albumin

Suggested Dilution: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (/).

Suggested Dilution: Flow Cytometry - Neat - 1/10 1. Higgins. L. M. et al. (1999) Regulation of T Cell Activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: Amelioration of Ongoing Inflammatory Bowel Disease with an OX40-IgG Fusion Protein. but not with an OX40 ligand-IgG fusion protein. 2. Takeda. K. et al. (2000) CD27-mediated activation of murine NK cells.