Dengue NS1 n

GenWay ID: GWB-7C7E10
Legacy ID: 10-663-45283
Source: E. Coli
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Details for Dengue NS1 n

Method: Dengue protein is purified by proprietary chromatographic technique. Useful Applications: Each laboratory should determine an optimum working titer for use in its particular application.

Description: The E.coli derived recombinant protein contains the NS1 Dengue Virus n-end Type-2 immunodominant regions. The Recombinant Dengue Virus NS1n is fused to a GST tag. The E.coli derived recombinant protein contains the NS1 Dengue Virus n-end Type-2 immunodominant regions.

Caused by one of four closely related virusserotypesof the genus Flavivirus, family Flaviviridae, each serotype is sufficiently different that there is no cross-protection and epidemics caused by multiple serotypes (hyperendemicity) can occur. In cell culture experiments and mice Morpholinoantisense oligos have shown specific activity against Dengue virus.

Function: Protein C packages viral RNA to form a viral nucleocapsid, and promotes virion budding (By similarity).

Function: prM acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide. prM is matured in the last step of virion assembly, presumably to avoid catastrophic activation of the viral fusion peptide induced by the acidic pH of the trans-Golgi network. After cleavage by host furin, the pr peptide is released in the extracellular medium and small envelope protein M and envelope protein E homodimers are dissociated (By similarity).

Function: Envelope protein E binds cell surface receptor and is involved in membrane fusion between virion and target cell. Synthesized as an homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity).

Function: Non-structural protein 1 is slowly secreted from mammalian cells, but not from mosquito cells. Secreted form elicits protective immune response and plays an essential role in RNA replication. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome (By similarity).

Subunit: prM and envelope protein E form heterodimers in the endoplasmic reticulum and Golgi. Envelope protein E forms homodimers. NS1 forms homodimers as well as homohexamers when secreted. NS1 may interact with NS4A (By similarity).

Subcellular Location: Protein C: Virion (By similarity).

Subcellular Location: Peptide pr: Secreted (By similarity).

Subcellular Location: Small envelope protein M: Virion membrane; Single-pass type I membrane protein (By similarity).

Subcellular Location: Envelope protein E: Virion membrane; Single-pass type I membrane protein (By similarity).

Subcellular Location: Non-structural protein 1: Secreted. Endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side (By similarity).

Domain: Transmembrane domains of the small envelope protein M and envelope protein E contains an endoplasmic reticulum retention signals (By similarity).

Ptm: Specific enzymatic cleavages in vivo yield mature proteins. The nascent protein C contains a C-terminal hydrophobic domain that act as a signal sequence for translocation of prM into the lumen of the ER. Mature protein C is cleaved at a site upstream of this hydrophobic domain by NS3. prM is cleaved in post-Golgi vesicles by a host furin, releasing the mature small envelope protein M, and peptide pr (By similarity).

Ptm: Envelope protein E and non-structural protein 1 are N-glycosylated (By similarity).

Miscellaneous: The virion is assembled in the endoplasmic reticulum lumen, transported by vesicles to the Golgi, then transported again to the cell membrane where it is released outside the cell.

Additional Info for Dengue NS1 n

Name Dengue NS1 n
Related Product Names Dengue Virus NS1n Recombinant; N/A Dengue NS1 n
Intended Use Research Use Only
Purity Dengue protein is >90% pure as determined by 10% PAGE (coomassie staining).
Format (1mg/ml) 100mM Tris-Hcl pH 8.0, 60mM NaCl, and 5% glycerol.
Storage Stable at 4C for 1 week. Should be stored below -18C. Prevent freeze/thawcycles
Molecular Weight 0.5
Swiss Prot Number P27913

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