Details for Human Serum Amyloid A (SAA) ELISA
The Human Serum Amyloid A (SAA) test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring SAA in serum or plasma samples of humans.
|Range of Standard Curve||10 ng/ml - 0.157 ng/ml|
|Total Assay Time||Total Incubation Time: 60 minutes|
PRINCIPLE OF THE ASSAY
The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the SAA present in samples reacts with the anti-SAA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-SAA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound SAA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of SAA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of SAA in the test sample. The quantity of SAA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Additional Info for Human Serum Amyloid A (SAA) ELISA
|Name||Human Serum Amyloid A (SAA) ELISA|
|Related Product Names||SAA, Serum amyloid A protein|
|NCBI Acc Number||P35541.2|
|Swiss Prot Number||P02735 (SAA_HUMAN)|
|Applications||A quantitative ELISA-based test kit for detecting Human SAA in biological samples.|