Details for Rat BDNF ELISA Kit
Application: For quantitative detection of rat BDNF in sera, plasma, body fluids, tissue lysates or cell culture supernates.
Specificity: No detectable cross-reactivity with any other cytokine.
Background: Brain-derived neurotrophic factor (BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor (NGF). BDNF and neurotrophin-3 (NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system.1 BDNF and other neurotrophins are critically involved in long-term potentiation (LTP). BDNF-mediated LTP is induced postsynaptically.2 BDNF has trophic effects on serotonergic (5-HT) neurons in the central nervous system.3 BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state.4 It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury.5 The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13. 6
Principle of ELISA: GenWay's rat BDNF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat BDNF specific-specific polyclonal antibodies were precoated onto 96-well plates. The rat specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat BDNF amount of sample captured in plate.
Expiration: Four months at 4C and eight months at -20C.
References: 1. Jones, K. R.; Reichardt, L. F. Molecular cloning of a human gene that is a member of the nerve growth factor family. Proc. Nat. Acad. Sci. 87: 8060-8064, 1990. 2. Kovalchuk, Y.; Hanse, E.; Kafitz, K. W.; Konnerth, A. Postsynaptic induction of BDNF-mediated long-term potentiation. Science 295: 1729-1734, 2002. 3. Lyons, W. E.; Mamounas, L. A.; Ricaurte, G. A; Coppola, V.; Reid, S. W.; Bora, S. H.; Wihler, C.; Koliatsos, V. E.; Tessarollo, L. Brain-derived neurotrophic factor-deficient mice develop aggressiveness and hyperphagia in conjunction with brain serotonergic abnormalities. Proc. Nat. Acad. Sci. 96: 15239-15244, 1999. 4. Rios, M.; Fan, G.; Fekete, C.; Kelly, J.; Bates, B.; Kuehn, R.; Lechan, R. M.; Jaenisch, R. Conditional deletion of brain-derived neurotrophic factor in the postnatal brain leads to obesity and hyperactivity. Molec. Endocr. 15: 1748-1757, 2001. 5. Baker-Herman, T. L.; Fuller, D. D.; Bavis, R. W.; Zabka, A. G.; Golder, F. J.; Doperalski, N. J.; Johnson, R. A.; Watters, J. J.; Mitchell, G. S. BDNF is necessary and sufficient for spinal respiratory plasticity following intermittent hypoxia. Nature Neurosci. 7: 48-55, 2004. 6. Maisonpierre, P. C.; Le Beau, M. M.; Espinosa, R., III; Ip, N. Y.; Belluscio, L.; de la Monte, S. M.; Squinto, S.; Furth, M. E.; Yancopoulos, G. D. Human and rat brain-derived neurotrophic factor and neurotrophin-3: gene structures, distributions and chromosomal localizations. Genomics 10: 558-568, 1991.
Additional Info for Rat BDNF ELISA Kit
|Name||Rat BDNF ELISA Kit|
|Related Product Names||Brain-derived neurotrophic factor, BDNF, Abrineurin,Bdnf ELISA Kit, Rat Brain-derived neurotrophic factor|
|Stability||Four months at 4C and eight months at -20C.|
|Storage||Store at 4C for frequent use, at -20C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Applications||For quantitative detection of rat BDNF in sera, plasma, body fluids, tissue lysates or cell culture supernates.|