GenWay ID: GWB-B7EE87
Legacy ID: 18-783-77317
Size: 1 ml
Source: Rabbit
Reactivity: Human
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Details for Anti- ERYTHROCYTE ANKYRIN Antibody


Isotype: Specificity: ERYTHROCYTE ANKYRIN Immunogen: Synthetic peptide corresponding to the amino acids 1 - 11 of the native protein conjugated to Keyhole Limpet Haemocyanin.

Specificity Note: This product recognizes erythroid Ankyrin (ankyrin-1), isoforms 2.1 and 2.2.

Ankyrins are a family of protein believed to link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and therefore aid cell motility, activation, proliferation and function. Most are composed of 3 structural domains; a N-terminal regulatory domain containing multiple ankyrin repeats, a central spectrin binding domain and a C-terminal regulatory domain. Ankyrin-1 is present in erythrocytes and has also been found in brain and muscle. It is suspected that mutations in erythrocytic ankyrin-1 are associated with hereditary spherocytosis. Preparation: Purified IgG prepared by affinity chromatography

Buffer Solution: Phosphate buffered saline

Preservative Stabilisers: 0.09%Sodium Azide (NaN3)

Suggested Dilution: Western Blot: In blots of human erythrocytes bands of 220 kDa were observed.

Suggested Dilution: ELISA - 1/10,000 - 1/100,000 Function: Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu17 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils.

Subunit: Interacts with a number of integral membrane proteins and cytoskeletal proteins. Binds SPTB/spectrin (beta chain) through a 70 AA N-terminal region of the 62 kDa domain, and the C-terminal of SLC4A1/erythrocyte membrane protein band 3 through the ankyrin repeat region. Also interacts with TTN/titin. Isoform Mu17 interacts with OBSCN isoform 3/obscurin.

Subcellular Location: Isoform Er1: Cytoplasm, cytoskeleton. Note=Probably the other erythrocyte (Er) isoforms, are located near the surface of erythrocytic plasma membrane.

Subcellular Location: Isoform Mu17: Membrane. Cytoplasm, myofibril, sarcomere, M-band. Note=Colocalizes with OBSCN isoform 3/obscurin at the M line in differentiated skeletal muscle cells.

Subcellular Location: Isoform Mu18: Sarcoplasmic reticulum (Probable).

Subcellular Location: Isoform Mu19: Sarcoplasmic reticulum (Probable).

Subcellular Location: Isoform Mu20: Sarcoplasmic reticulum (Probable).

Tissue Specificity: Isoform Mu17, isoform Mu18, isoform Mu19 and isoform Mu20 are expressed in skeletal muscle. Isoform Br21 is expressed in brain.

Domain: The 55 kDa regulatory domain is involved in regulating binding of SPTB/spectrin (beta chain) and SLC4A1/erythrocyte membrane protein band 3.

Domain: The ANK repeat region forms a spiral around a large central cavity and is involved in binding of ion transporters.

Ptm: Regulated by phosphorylation.

Ptm: Palmitoylated.

Disease: Defects in ANK1 are a cause of hereditary spherocytosis (HS) [MIM:182900]. Inheritance can be autosomal dominant or recessive.

Similarity: Contains 23 ANK repeats.

Similarity: Contains 1 death domain.

Similarity: Contains 1 ZU5 domain.

Additional Info for Anti- ERYTHROCYTE ANKYRIN Antibody

NCBI Acc Number NP_000028.3
Format Purified
Stability 18 months from date of despatch.
Clonality Polyclonal
Storage Store at +4 degree C or at -20 degree C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a pr
Molecular Weight 206265
Applications ELISA, WB

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