Clone: 2G2

Isotype: IgG1

Immunogen: Recombinant full length protein (Human).

Antigen Species: Human Target: Mitochondrial p110

Localization: Mitochondrial

Concentration: 1 mg/ml Storage Buffer: Preservative: 0.08% Sodium Azide; Constituents: PBS. pH 7.4

Application Note: IF: Use at an assay dependent dilution. WB: Use at a concentration of 2 - 10 µg/ml. Detects a band of approximately 110 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular Localization: Mitochondrial p110 has an approximate isoelectric point of 6.5 and co-partitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. Close examination of the staining pattern in HeLa and Fanconiís anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconiís anemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules.