Specificity: CD19

Isotype: IgG1

Buffer Solution: Phosphate buffered saline pH7.4 Preservative Stabilisers: 0.09% Sodium Azide 1% Bovine Serum Albumin

Specificity: This antibody recognises the human CD19 cell surface antigen a 96kD single chain glycoprotein on the surface of human B lineage cells, including pre-B cells. B cells can be detected in peripheral blood and tissues. Specificity: Labels all B lymphocytes in peripheral blood and spleen but is negative on granulocytes, monocytes, platelets, T lymphocytes and erythrocytes.
This antibody is useful for enumerating normal B lymphocytes and typing B cell malignancies by flow cytometry and immunofluorescence.
The antibody reacts with most B cell malignancies including chronic leukaemia and most lymphomas, but not multiple myeloma. Recommended Negative Controls: MOUSE IgG1 NEGATIVE CONTROL:RPE Function: Assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Subunit structureForms a complex with CD21, CD81 and CD225 in the membrane of mature B cells. Interacts with VAV. Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409. Ref.10

Subcellular locationMembrane; Single-pass type I membrane protein.

Post-translational modificationPhosphorylated on serine and threonine upon DNA damage, probably by ATM or ATR. Phosphorylated on tyrosine following B-cell activation. Ref.10Ref.9Ref.11

Involvement in diseaseDefects in CD19 are a cause of hypogammaglobulinemia [MIM:107265]. Ref.12

Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.

Sequence cautionThe sequence AAA35533.1 differs from that shown. Reason: Frameshift at position 396. [1] \"Bmf: a proapoptotic BH3-only protein regulated by interaction with the myosin V actin motor complex, activated by anoikis.\"Puthalakath H., Villunger A., O\'Reilly L.A., Beaumont J.G., Coultas L., Cheney R.E., Huang D.C.S., Strasser A.Science 293:1829-1832(2001) [PubMed: 11546872] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: T-cell. [2] \"Expression and transcriptional regulation of functionally distinct Bmf isoforms in B-chronic lymphocytic leukemia cells.\"Morales A.A., Olsson A., Celsing F., Oesterborg A., Jondal M., Osorio L.M.Leukemia 18:41-47(2004) [PubMed: 14574334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), TISSUE SPECIFICITY. Tissue: B-cell. [3] \"Characterization of long cDNA clones from human adult spleen.\"Hattori A., Okumura K., Nagase T., Kikuno R., Hirosawa M., Ohara O.DNA Res. 7:357-366(2000) [PubMed: 11214971] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Spleen. [4] \"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).\"The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Placenta.