Clone ID: EP1845Y

Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human Na+/K+ ATPase was used as an immungen. Specificity: A synthetic peptide corresponding to residues near the N-terminus of human Na+/K+ ATPase α was used as an immungen. Dilution: WB: 1:10,000 - 50,000
IHC: 1:100 250
FC: 1:20. Na+, K+-ATPase is a heterodimeric enzyme responsible for the active maintenance of sodium and potassium gradients across the plasma membrane (1). The sodium- and potassium-dependent adenosine triphosphatase (Na+,K(+)-ATPase) maintains the transmembrane Na+ gradient to which is coupled all active cellular transport systems (2). It is well accepted that inhibition of the Na,K-ATPase in the heart, through effects on the Na/Ca exchanger, raises the intracellular Ca2+ concentration and strengthens cardiac contraction. Assessing the phenotypes of mouse hearts with genetically reduced levels of Na,K-ATPase alpha 1 or alpha 2 isoforms clearly demonstrates different functional roles for these isoforms in vivo. Results definitively illustrate a specific role for the alpha 2 Na,K-ATPase isoform in Ca2+ signaling during cardiac contraction (3). 1. Kada S, Nakagawa T, Ito J, A Mouse Model for Degeneration of the Spiral Ligament ,J Assoc Res Otolaryngol 10 (2009) 161-172
[Application: IHC]
2. Daley WP, Gulfo KM, Sequeira SJ, et al, Na+/K+ ATPase ,Dev Biol 336 (2009) 169-182
[Application: ICC]
1. Yang-Feng TL, et al. Genomics 2(2):128-38, 1988
2. Herrera VL, et al. Science 249(4972):1023-6, 1990
3. James PF, et al. Mol Cell 3(5):555-63, 1999.
4. Manufactured under U.S. Patents, No. 5,675,063 and 7,429,487