Details for NUCLEAR ANTIGEN, Antibody
Specificity: NUCLEAR ANTIGEN
Clone: 21NC85 Immunogen: Native
Specificity Note: This product is histone specific, it stains nuclei and chromosomes of cells from all species including animals and plants. It reacts preferentially with histone H1 by ELISA and immunoblotting. Stains nuclei of all human cell types and also stains chromosomes diffusely in metaphase cells. Intense diffuse staining is obtained in interphase cells.
Target Species: Human
Histology: Human Tonsil
Preparation: Ig fraction prepared by ammonium sulphate precipitation
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Suggested Dilution: Suggested Dilution: Immunofluorescence - 1/20 - 1/30 Function: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase\'s processibility during elongation of the leading strand.
Subunit: Homotrimer. Interacts with KCTD10. Interacts with PPP1R15A (By similarity). Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with POLH, POLK, DNMT1, ERCC5/XPG, FEN1, CDC6, APEX2 and POLDIP2. Interacts with EXO1 and SHPRH. Forms a ternary complex with DNTTIP2 and core histone. Interacts with POLD1, POLD3 and POLD4.
Subcellular Location: Nucleus.
Ptm: Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical poly-ubiquitination on Lys-164 through \'Lys-63\' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligase SHPRH, whih is required for DNA repair.
Disease: Antibodies are present in sera from patients with systemic lupus erythematosus.
Similarity: Belongs to the PCNA family.
Additional Info for NUCLEAR ANTIGEN, Antibody
|Name||NUCLEAR ANTIGEN, Antibody|
|Related Product Names||MOUSE ANTI HUMAN NUCLEAR ANTIGEN NUCLEAR ANTIGENPCNA|
|NCBI Acc Number||NP_002583.1|
|Purity||Purified IgG prepared by affinity chromatography|
|Format||IgG Fraction - liquid|
|Stability||18 months from date of despatch.|
|Storage||Store at -20°C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted.|
|Applications||IHC-F, ELISA, IF, IHC-P, WB|