PGP 9.5, Antibody

GenWay ID: GWB-8A9363
Legacy ID: 20-511-240163
Size: 0.2 ml
Source: Mouse
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Details for PGP 9.5, Antibody

Isotype: IgG2a

Clone: 13C4

Host Animal: Mouse

Immunogen: Native from brain

Specificity: Reacts with PGP 9.5 from human. rat and guinea pig. Stains neuronal cell bodies and axons in the CNS and periphery. small nerve fibers in peripheral tissues. neuroendocrine cells in the pituitary. thyroid. pancreas and tumors of the DNES. Also stains neuroendocrine cells in human adult gut. Type of Product: Monoclonal Antibodies for Neuroscience

Concentration: 1mg/ml (OD280nm)

Preservatives: NaN3

Buffer: PBS, pH 7.2

Applications Notes : Suitable for use in Immunohistochemistry using paraffin sections (1:50–1:400) and ELISA (1:160,000) (EPT). Simple ELISA. Native protein coated at 100ng/well. Suitable for use on paraffin embedded tissue sections. We do recommend fixation in 95% ethanol/5% acetic acid for 2–3 hours prior to paraffin embedding. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. Warning: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1–1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains. MAb to PGP 9.5. Monoclonal Antibody to Protein Gene Product 9.5 (PGP 9.5)

Function: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Catalytic Activity: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Biophysicochemical Properties:

Subunit: Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5.

Subcellular Location: Cytoplasm.

Tissue Specificity: Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary.

Ptm: O-glycosylated (By similarity).

Disease: Oxidation of Met-1, Met-6, Met-12, Met-124 and Met-179 to methionine sulfoxide, and oxidation of Cys-220 to cysteine sulfonic acid have been observed in brains from Alzheimer disease (AD) and Parkinson disease (PD) patients. In AD, UCHL1 was found to be associated with neurofibrillary tangles.

Miscellaneous: In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.

Similarity: Belongs to the peptidase C12 family [view classification].

Additional Info for PGP 9.5, Antibody

Name PGP 9.5, Antibody
Related Product Names MAb to PGP 9.5; UCH-L1; EC; EC 6.-.-.-; Ubiquitin thioesterase L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5 PGP 9.5UCHL1
NCBI Acc Number NP_004172.2
Purity Protein A chromatography
Format Purified, Liquid
Clonality Monoclonal
Storage Upon receipt, aliquot and store at -20 C. Avoid multiple freeze-thaw cycles.
Molecular Weight 24824
Swiss Prot Number P09936
Applications ELISA, IHC-P

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