EDNRB antibody, Endothelin B receptor Antibody, Rabbit Polyclonal Antibody

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Endothelin B receptor Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-461-10577

Buy EDNRB antibody -

Volume:

Related Product Names:

- EDNRB antibody; EDNRB; ETRB; ETB; HSCR; ABCDS; Endothelin B receptor
- ET-B; Endothelin receptor Non-selective type; Endothelin receptor type B isoform 1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: EDNRB	Gene Name Synonym: ETRB; ETB; HSCR; ABCDS
Gi #: 4557547	NCBI Acc #: NP_000106.1	Swiss Prot Acc #: P24530
Length (aa): 442	Mol. Weight (Da): 49644	Chrom Location: 13q22

Immunogen: N-terminal extracellular domain of human. Synthetic peptide - KLH conjugated.

Family: GPCR / Endothelin

Eptiope: N-terminal extracellular domain of human
Mouse Homology: 21% (identity) / 21% (Similarity)
Rat Homology: 21% (identity) / 21% (Similarity)

Uses: WB, IHC (4 - 11 ug/ml), ICC (Optimal dilution to be determined by the researcher)

Summary: The Endothelin Receptor ETB is expressed in most tissues. Mutations of the receptor gene are responsible for Hirschsprung disease type 2, a multigenic disorder with phenotypes such as bicolored irides, megacolon, hypopigmentation, and hearingloss. The ETB receptor has similar affinity for all three endothelins and activates a phosphatidylinositol-calcium second messenger system. A splice variant, termed SVR, has been described; the sequence of the ETB-SVR receptor is identical toET-B receptor, with the exception of the intracellular C-terminal domain. While both splice variants bind endothelin ET1, they exhibit different responses, which suggests that they may be functionally distinct.

Source/Host: Rabbit

Purity/Purification: Peptide immunogen affinity column

Clonality: Polyclonal

Crossreactivity: Human

Format: Phosphate buffered saline, pH 7.7, containing 0.1% sodium azide.

Storage: Long term: -70C; Short term: +4C

Stability: 6-12 months at -20 degree C.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for EDNRB ANTIBODY:

ICC, IHC: Tested

ELISA, Western Blot: Not Tested

TESTING: (secondary reagents and protocols )

IHC: Adrenal, Medulla ( anti-EDNRB )

EDNRB antibody IHC image
EDNRB

ICC: Transfection - cells expressing target protein ( anti-EDNRB )

EDNRB antibody ICC image
EDNRB

ICC: Transfection - cells expressing vector only ( anti-EDNRB )

EDNRB antibody ICC image
EDNRB

IHC: 18-461-LS-A54_353093a ( anti-EDNRB )

EDNRB ANTIBODY TARGET DESCRIPTION:

Synonym Names for EDNRB antibody: EDNRB; ETRB; ETB; HSCR; ABCDS; ET-B; Endothelin receptor Non-selective type; Endothelin receptor type B isoform 1

Function: Non-specific receptor for endothelin 1, 2, and 3. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subcellular Location: Membrane; multi-pass membrane protein.

Tissue Specificity: Expressed in placental stem villi vessels, but not in cultured placental villi smooth muscle cells.

Ptm: Palmitoylation of Cys-402 was confirmed by the palmitoylation of Cys-402 in a deletion mutant lacking both Cys-403 and Cys-405.

Disease: Defects in EDNRB are a cause of Waardenburg syndrome type IV (WS4) [MIM:277580]; also known as Waardenburg-Shah syndrome. WS4 is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).

Disease: Defects in EDNRB are the cause of Hirschsprung disease type 2 (HSCR2) [MIM:600155]; also known as aganglionic megacolon (MGC). It is a congenital disorder characterized by absence of enteric ganglia along a variable length of the intestine. It is the most common cause of congenital intestinal obstruction. Early symptoms range from complete acute neonatal obstruction, characterized by vomiting, abdominal distention and failure to pass stool, to chronic constipation in the older child.

Disease: Defects in EDNRB are the cause of ABCD syndrome [MIM:600501]. ABCD syndrome is an autosomal recessive syndrome characterized by albinism, black lock, cell migration disorder of the neurocytes of the gut (Hirschsprung disease), and deafness.

Similarity: Belongs to the G-protein coupled receptor 1 family [view classification].

Summary: Endothelin receptor type B is a G protein-coupled receptor which activates a phosphatidylinoitol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutation in endothelin receptor type B gene. A splice variant, named SVR, has been described; the sequence of the ETB-SVR receptor is identical to ETRB except for the intracellular C-terminal domain. While both splice variants bind ET1, they exhibit different responses upon binding which suggests that they may be functionally distinct.

Endothelin B receptor reacts with human.

OMIM: 131244; gene. [NCBI / EBI]
142623; phenotype. [NCBI / EBI]
277580; phenotype. [NCBI / EBI]
600155; phenotype. [NCBI / EBI]
600501; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Calcium signaling pathway 04020
KEGG pathway: Neuroactive ligand-receptor interaction 04080

Products similar to EDNRB antibody:

IgG

GPR37L1, IgG

Endothelin B receptor, IgG

Endothelin B receptor-like protein 2, IgG

GPR37L1, IgG

Rabbit anti Endothelin B Receptor, IgG

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for EDNRB ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Shirai,N., Naruko,T., Ohsawa,M., Ikura,Y., Sugama,Y., Hirayama,M., Kitabayashi,C., Ehara,S., Inoue,T., Itoh,A., Haze,K., Tanzawa,K.,, et al.
     Expression of endothelin-converting enzyme, endothelin-1 and endothelin receptors at the site of percutaneous coronary intervention in humans
[2] Chen,S.C., Lin,C.Y., Chen,Y.H., Fang,H.Y., Cheng,C.Y., Chang,C.W., Chen,R.A., Tai,H.L., Lee,C.H., Chou,M.C., Lin,T.S. and Hsu,L.S.
     Aberrant promoter methylation of EDNRB in lung cancer in Taiwan
[3] Gormley,K., Bevan,S., Hassan,A. and Markus,H.S., et al.
     Polymorphisms in genes of the endothelin system and cerebral small-vessel disease
[4] Anguelova,E., Beuvon,F., Leonard,N., Chaverot,N., Varlet,P., Couraud,P.O., Daumas-Duport,C. and Cazaubon,S.
     Functional endothelin ET B receptors are selectively expressed in human oligodendrogliomas
[5] Laghmani,K., Sakamoto,A., Yanagisawa,M., Preisig,P.A. and Alpern,R.J.
     A consensus sequence in the endothelin-B receptor second intracellular loop is required for NHE3 activation by endothelin-1
[6] , Nakamuta M., Takayanagi R., Sakai Y., Sakamoto S., Hagiwara H., Mizuno T., Saito Y., Hirose S., Yamamoto M., Nawata H., et al.
     Cloning and sequence analysis of a cDNA encoding human non-selective type of endothelin receptor.
[7] , Ogawa Y., Nakao K., Arai H., Nakagawa O., Hosoda K., Suga S., Nakanishi S., Imura H.
     Molecular cloning of a non-isopeptide-selective human endothelin receptor.
[8] , Arai H., Nakao K., Hosoda K., Ogawa Y., Nakagawa O., Komatsu Y., Imura H.
     Molecular cloning of human endothelin receptors and their expression in vascular endothelial cells and smooth muscle cells.
[9] , Arai H., Nakao K., Takaya K., Hosoda K., Ogawa Y., Nakanishi S., Imura H.
     The human endothelin-B receptor gene. Structural organization and chromosomal assignment.
[10] , Sakamoto A., Yanagisawa M., Sakurai T., Takuwa Y., Yanagisawa H., Masaki T.
     Cloning and functional expression of human cDNA for the ETB endothelin receptor.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: EDNRB antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of EDNRB antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about EDNRB antibody, all related information, articles, resources about EDNRB antibody are being stored on our online database. Let us know if you have questions regarding this product.

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