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| Multidrug Resistance-related Protein MRP2 (cMOAT) Antibody, Mouse Monoclonal Antibody |
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| Catalog Number: 20-881-120009 |
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| Related Product Names:
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ABCC2 antibody; ABCC2; CMOAT; CMOAT1; CMRP; MRP2; Multidrug Resistance-related Protein MRP2 (cMOAT)
- Monoclonal antibody to the Multidrug Resistance-related Protein MRP2 (cMOAT); ATP-binding cassette sub-family C member 2; Multidrug resistance-associated protein 2; Canalicular multidrug resistance protein; Canalicular multispecific organic anion transporter 1 |
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- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
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| Gene Name: ABCC2 |
Gene Name Synonym: CMOAT; CMOAT1; CMRP; MRP2 |
Gi #:
N/A |
NCBI Acc #:
N/A |
Swiss Prot Acc #:
Q92887 |
Length (aa): N/A |
Mol. Weight (Da): 174191 |
Chrom Location: N/A |
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 Clone: M2I-4
Ig Subclass: IgG1
Specificity: M2I-4 reacts with an internal epitope of MRP2, a 190-200 kD transmembrane protein earlier known as the canalicular multi-organic anion transporter cMOAT, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines. M2I-4 was raised against a bacterial fusion protein of MRP2, containing amino acids 215-310 of the protein. M2I-4 did not cross-react with the human MDR1, MRP1, MRP3 and MRP5 gene products
Mab Producing Cells: The hybridoma cell line was obtained by fusion of lymph node cells from an immunized mouse (Balb/c) with SP2/O mouse myeloma cells
Uses: M2I-4 has potential value for detection of MRP2-mediated drug-resistance in human tumor samples. Immunocytochemistry: use 1:20-50 dil. on acetone fixed cytospin preparations. For immunohistochemistry: M2I-4 (use 1:20) on acetone fixed frozen sections can be followed by incubation with rabbit anti- mouse IgG (1:25, Dako) and a monoclonal mouse APAAP complex (1:50, Dako). After incubation of M2I-4 (use 1:20) and washing, slides can be incubated with biotinylated rabbit anti-mouse IgG (1:100, Jackson, West Grove) and streptavidin conjugated to horseradish peroxidase (1:500, Zymed, San Francisco, CA). Flow cytometry: optimal conditions still to be established. Western blotting: use 1:20-50 dil, and anti-mouse-HRP
Culture Medium: IMDM (BioWhittaker), supplemented with Nutridoma-SP (Boehringer, Indianapolis, USA), without serum or added enzymes. Antibody containing supernatant has been concentrated and filtered through a 0.22 micron filter
Note: this monoclonal antibody has been produced in a clinical laboratory in which no animal viruses are being studied or cultured
Source/Host: Mouse
Clonality: Monoclonal
Format: 1 ml vials (>200 tests) containing antibody in serumfree culture supernatant, with 0.7% BSA (Roche, Mannheim, Germany) and 0.1% Sodium azide. Concentration 250 µg immunoglobulin/ml (by ELISA)
Storage: Ship in ambient temperature. Store 4 degee C. For extended storage, Freeze at -20 degree C.
Shipping: Products may be shipped on ice pack or dry ice. |
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| ABCC2 ANTIBODY TARGET DESCRIPTION: |
Synonym Names for ABCC2 antibody: ABCC2; CMOAT; CMOAT1; CMRP; MRP2; Monoclonal antibody to the Multidrug Resistance-related Protein MRP2 (cMOAT); ATP-binding cassette sub-family C member 2; Multidrug resistance-associated protein 2; Canalicular multidrug resistance protein; Canalicular multispecific organic anion transporter 1
Function: Mediates hepatobiliary excretion of numerous organic anions. May function as a cellular cisplatin transporter.
Subcellular Location: Membrane; Multi-pass membrane protein.
Tissue Specificity: Found on the apical membrane of polarized cells in liver, kidney and intestine. The highest expression is found in liver.
Disease: Defects in ABCC2 are the cause of Dubin-Johnson syndrome (DJS) [MIM:237500]. DJS is an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin-like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.
Similarity: Belongs to the ABC transporter family. Conjugate transporter (TC 3.A.1.208) subfamily [view classification].
Similarity: Contains 2 ABC transmembrane type-1 domains.
Similarity: Contains 2 ABC transporter domains.
OMIM: 237500; phenotype. [NCBI / EBI]
601107; gene. [NCBI / EBI]
Products similar to ABCC2 antibody:
IgG
 MRP3, IgG
MRP3 [M3II-21], IgG
Multidrug Resistance-related Protein MRP2/cMOAT, IgG
Multidrug Resistance Protein 2, MRP2/cMOAT, IgG
MRP2 [M2 III-6], IgG
Protein
MIP-1 GAMMA, Protein
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SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
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| BACKGROUND
REFERENCES for ABCC2 ANTIBODY: |
| Background references for antibody target are not specific to GenWay products |
| * Paulusma et al. Congenital jaundice in rats with a mutation in a multidrug resistance- associated protein gene. Science 271: 1126-1128, 1996!* Kool et al. Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologs of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines. Cancer Res 57: 3537-3547, 1997.!* Scheffer et al. Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5 and MDR3 P-glycoprotein with a panel of monoclonal antibodies. Cancer Res 60: 5269, 2000 |
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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: ABCC2 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of ABCC2 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about ABCC2 antibody, all related information, articles, resources about ABCC2 antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
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Thank you for visiting GenWay Biotech's Datasheet. GenWay is the world's leader in chicken, IgY antibody production, producing over 900 quality polyclonal IgY antibodies to date. From our unique recombinant protein production, utilizing a variety of protein expression systems, GenWay's polyclonal IgY antibodies incorporate more epitopes, increasing the success rate for ELISA, western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), and many more immunoassays. |
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