Mouse Fibrinogen, ELISA Kit

Alphabetical Listing of Primary Antibodies:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Alphabetical Listing of Proteins:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Peptides \| Secondary Antibodies \| ELISA Kits

Catalog Number: 40-374-130050

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Product Name: Mouse Fibrinogen

Sample Type: Serum/Plasma

# of Samples: 1X96 Assays

Intended Use: The Fibrinogen test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Fibrinogen in biological samples of mice.

Introduction: Soluble Fibrinogen (FIB) circulates in the blood and provides the material from which the insoluble fibrin clot is formed during blood coagulation. Fibrinogen is an acute phase reactant that may be a useful marker for infection and inflammation. This ELISA kit can be used to measure Fibrinogen in biological samples of mice.

Principles of the assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the Fibrinogen present in serum sample reacts with the anti-Fibrinogen. antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-Fibrinogen. antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum Fibrinogen. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Fibrinogen in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Fibrinogen in the test sample. The quantity of Fibrinogen in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.

Storage and Stability: The expiration date for the package is stated on the box label.
1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8c.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16-25C) or at 4-8c.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-Fibrinogen conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for one day.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. FIBRINOGEN ELISA Micro plate
Anti-Mouse Fibrinogen coated wells are stable until the expiration date, and should be stored at 4-8C in the sealed foil pouch with desiccant pack.
7. FIBRINOGEN CALIBRATOR
Mouse Fibrinogen calibrator should be aliquoted out and stored frozen. For storage longer than 14 days, keep frozen until the expiration date. Storage of less than 14 days can be 4C. Avoid multiple freeze-thaw cycles. The working standard solutions are stable for 12 hours.
INDICATIONS OF INSTABILITY
If the test is performing correctly, the results observed with the standard solutions should be within 20 % of the expected values.

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