LGALS3 antibody, GALECTIN-3 Antibody, Mouse IgG1 Monoclonal Antibody

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GALECTIN-3 Antibody, Mouse IgG1 Monoclonal Antibody

Catalog Number: 20-321-175224

Buy LGALS3 antibody -

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Related Product Names:

- LGALS3 antibody; LGALS3; MAC2; GALECTIN-3
- MONOCLONAL ANTIBODY TO HUMAN GALECTIN-3; Galactose-specific lectin 3; Mac-2 antigen; IgE-binding protein; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Laminin-binding protein; Lectin L-29; L-31; Galactoside-binding protein; GALBP; Galectin 3; Galectin-3

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: LGALS3	Gene Name Synonym: MAC2
Gi #: 115430223	NCBI Acc #: NP_002297.2	Swiss Prot Acc #: P17931
Length (aa): 250	Mol. Weight (Da): 26188	Chrom Location: N/A

Clone: B2C10

Applications: The monoclonal antibody B2C10 can be used for Western blotting, flow cytometry and immunohistology on both frozen and paraffin embedded sections. Furthermore the monoclonal antibody B2C10 is useful for immuno assays and functional studies.

Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies dilutions have to be made according to the amounts of galectin-3 to be inhibited.

Source/Host: Mouse IgG1

Clonality: Monoclonal

Crossreactivity: Human

Format: Filtered antibody solution in PBS. containing 0.1% bovine serum albumin.

Storage: Product should be stored at 4C. Under recommended storage conditions, product is stable for one year.

Stability: Stable for 1 year

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for LGALS3 ANTIBODY:

WB, FACS/FC, IHC-F, IHC-P: Tested

TESTING: (secondary reagents and protocols )

Not Available

LGALS3 ANTIBODY TARGET DESCRIPTION:

Synonym Names for LGALS3 antibody: LGALS3; MAC2; MONOCLONAL ANTIBODY TO HUMAN GALECTIN-3; Galactose-specific lectin 3; Mac-2 antigen; IgE-binding protein; 35 kDa lectin; Carbohydrate-binding protein 35; CBP 35; Laminin-binding protein; Lectin L-29; L-31; Galactoside-binding protein; GALBP; Galectin 3; Galectin-3

The monoclonal antibody B2C10 reacts with galectin-3. a 30 kDa protein. Galectin-3 is a member of the galectin family. The protein is composed of three domains: a small amino-terminal domain. a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal domain containing repeating elements. Galectin-3 is normally distributed in epithelia of many organs and various inflammatory cells. including macrophages. as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation. cell proliferation. cell differentiation and through trans-activation by viral proteins. The expression is also affected by neoplastic transformation: up-regulated in certain types of lymphomas and thyroid carcinoma. while down-regulated in other types of malignancies. such as colon. breast. ovarian and uterine carcinomas. Galectin-3 has been shown to function through both intracellular and extracellular actions. Related to its intracellular functions. galectin-3 has been identified as a component of heterogeneous nuclear ribonuclear protein (hnRNP). a factor in pre-mRNA splicing. and has been found to control cell cycle and prevent T cell apoptosis. On the other hand. this protein has also been demonstrated to function as extracellular molecule in activating various types of cells. including monocytes/macrophages. mast cells. neutrophils and lymphocytes. Galectin-3 has been shown to mediate cell-cell and cell-extracellular matrix interactions. The monoclonal antibody B2C10 inhibits the binding of 125I-labeled galectin-3 to IgE coated on microtiter plates. the galecin-3's hemagglutination activity and galectin-3-induced superoxide production by human neutrophils. This inhibitory activity of B2C10 is probably the result of its disruption of the self-association process. The epitope of the monoclonal antibody B2C10 is found within the first 45 amino acids of galectin-3. The antibody B2C10 does not react with Galecin-3C and is cross reactive with mouse galectin-3.

Function: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration.

Subunit: Probably forms homo- or heterodimers. Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3 and UACA.

Subcellular Location: Nucleus. Note=Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.

Tissue Specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.

Similarity: Contains 1 galectin domain.

GALECTIN-3 reacts with human.

OMIM: 153619; gene. [NCBI / EBI]

Products similar to LGALS3 antibody:

IgG

IGFBP7, IgG

Galectin-3-binding protein, Protein

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for LGALS3 ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Seidel,R.D. III, Zhuang,T. and Prestegard,J.H., et al.
     Bound-state residual dipolar couplings for rapidly exchanging ligands of His-tagged proteins
[2] Mina-Osorio,P., Soto-Cruz,I. and Ortega,E., et al.
     A role for galectin-3 in CD13-mediated homotypic aggregation of monocytes
[3] Nieminen,J., Kuno,A., Hirabayashi,J. and Sato,S., et al.
     Visualization of galectin-3 oligomerization on the surface of neutrophils and endothelial cells using fluorescence resonance energy transfer
[4] Yu,L.G., Andrews,N., Zhao,Q., McKean,D., Williams,J.F., Connor,L.J., Gerasimenko,O.V., Hilkens,J., Hirabayashi,J., Kasai,K., et al.
     Galectin-3 interaction with Thomsen-Friedenreich disaccharide on cancer-associated MUC1 causes increased cancer cell endothelial adhesion
[5] Nakahara,S., Hogan,V., Inohara,H. and Raz,A., et al.
     Importin-mediated nuclear translocation of galectin-3
[6] Robertson M.W., Albrandt K., Keller D., Liu F.-T.
     Human IgE-binding protein: a soluble lectin exhibiting a highly conserved interspecies sequence and differential recognition of IgE glycoforms.
[7] Cherayil B., Chaitovitz S., Wong C., Pillai S.
     Molecular cloning of a human macrophage lectin specific for galactose.
[8] Oda Y., Leffler H., Sakakura Y., Kasai K., Barondes S.H.
     Human breast carcinoma cDNA encoding a galactoside-binding lectin homologous to mouse Mac-2 antigen.
[9] Raz A., Carmi P., Raz T., Hogan V., Mohamed A., Wolman S.R.
     Molecular cloning and chromosomal mapping of a human galactoside-binding protein.
[10] Lotz M.M., Andrews C.W. Jr., Korzelius C.A., Lee E.C., Steele G.D. Jr., Clarke A., Mercurio A.M.
     Decreased expression of Mac-2 (carbohydrate binding protein 35) and loss of its nuclear localization are associated with the neoplastic progression of colon carcinoma.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: LGALS3 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of LGALS3 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about LGALS3 antibody, all related information, articles, resources about LGALS3 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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