GPT protein, Human Alanine Aminotransferase (r-h-ALT) recombinant protein

Alphabetical Listing of Primary Antibodies:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Alphabetical Listing of Proteins:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Peptides \| Secondary Antibodies \| ELISA Kits

Human Alanine Aminotransferase (r-h-ALT) recombinant protein

Catalog Number: 10-611-183005

Buy GPT protein -	Size:
Buy GPT protein -	Units:

Related Product Names:

- GPT protein; GPT; AAT1; GPT1; Human Alanine Aminotransferase (r-h-ALT)
- ALT1; EC 2.6.1.2; Glutamic--pyruvic transaminase 1; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Alanine aminotransferase 1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: GPT	Gene Name Synonym: AAT1; GPT1
Gi #: N/A	NCBI Acc #: NP_005300.1	Swiss Prot Acc #: P24298
Length (aa): N/A	Mol. Weight (Da): 54637	Chrom Location: N/A

Crossreactivity: Human

Shipping: Products may be shipped on ice pack or dry ice.

TESTING: (secondary reagents and protocols )

Not Available

GPT PROTEIN TARGET DESCRIPTION:

Synonym Names for GPT protein: GPT; AAT1; GPT1; ALT1; EC 2.6.1.2; Glutamic--pyruvic transaminase 1; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Alanine aminotransferase 1

The DNA encoding Human ALT is cloned from the human liver cDNA library. harboring the same amino acid sequence as the native cytosolic ALT ananlyzed by Ishiguro er al. r-hALT is expressed as an active holoenzyme in E. coli. r-hALT is purified to homogeneity as judged by SDS-PAGE. r-hALT is indistinguishable from native one in terms of molecular and catalytic properties. 1) Molecular size is 55 kDa on SDS-PAGE and 101 kDa on gel filtration. indicating a homodimer of two identical subunits. 2) Isoelectric Point is 6.5 when measured by isoelectric focusing. 3) Km value is measured to be 20.5 mM for L-Ala. and 0.443 mM for 2-oxoglutarate. 4) r-hALT shows high compatibility to native ALT on serum ALT assay in clinical chemistry tests.

Function: Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles.

Catalytic Activity: L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactor: Pyridoxal phosphate.

Pathway: Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.

Subunit: Homodimer.

Subcellular Location: Cytoplasm.

Tissue Specificity: Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.

Induction: By glucocorticoids.

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Human Alanine Aminotransferase (r-h-ALT) reacts with human.

OMIM: 138200; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Alanine and aspartate metabolism 00252
KEGG pathway: Carbon fixation 00710
KEGG pathway: Glutamate metabolism 00251
Reactome Event:Metabolism of amino acids 71291

Products similar to GPT protein:

IgG

SLC5A4 (solute carrier family 5 (low affinity glucose cotransporter). member 4), IgG

Anti-Angiopoietin 2 (Mouse specific), IgG

ANGPTL3 (clone 1D10), IgG

Anti-ALANINE TRANSAMINASE (ALT) (Pig Heart), IgG

Angptl4 (mouse), IgG

Native_Protein

GLUTAMIC PYRUVIC TRANSAMINASE, Native_Protein

Glut. Pyruvate Transaminase, Native_Protein

ANGPTL-4 Human, Protein

ANG-1 Human, Protein

39S ribosomal protein L28, mitochondrial, Protein

SUGGESTED PROTEIN REAGENTS - GenWay offers reagents to facilitate your research with cell-based assays, Mass Spectrometry, standards, calibrators, Western blot, cell culture, antibiotics, microwell substrates, and more!

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: GPT protein is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of GPT protein vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about GPT protein, all related information, articles, resources about GPT protein are being stored on our online database. Let us know if you have questions regarding this product.

Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.

GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.

GenWay has a diverse line of recombinant protein expression platforms, including E. coli expression, Bacliovirus expression, yeast expression, and mammalian expression. Combining the technologies of bioinformatics, protein domain analysis, gene codon optimization, protein fusion, and protein expression in different host cells, GenWay can produce soluble and functional proteins. In particular, GenWay has the capability and vector systems for expression of difficult proteins such as proteases, kinases, glycosylated and membrane proteins. We have a very high success rate (almost 100%) and can deliver the proteins in a timely fashion. This is a core competency necessary for efficient and effective production of immunogens and affinity ligands for antibody production and purification. GenWay has over 300

recombinant proteins

offered in our catalog.



GenWay Biotech, Inc. 6777 Nancy Ridge Drive, San Diego, CA 92121 Tel: (858)458-0866 Fax: (858)458-0833 Email: sales@genwaybio.com www.genwaybio.com ©1998 - 2010 GenWay Biotech, Inc. - All Rights Reserved