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| FAS [Alf2.1] Antibody, Mouse Monoclonal Antibody |
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| Catalog Number: 20-272-190231 |
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| Related Product Names:
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FAS antibody; FAS; 355; FAS; 134637; P25445; ALPS1A; APO-1; APT1; Apo-1 Fas; CD95; FAS1; FASTM; TNFRSF6;; FAS [Alf2.1]
- Mouse monoclonal [Alf2.1] to Fas Ligand; 355; FAS; 134637; P25445; ALPS1A; APO-1; APT1; Apo-1 Fas; CD95; FAS1; FASTM; TNFRSF6;; Tumor necrosis factor receptor superfamily member 6 |
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- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
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| Gene Name: FAS |
Gene Name Synonym: 355; FAS; 134637; P25445; ALPS1A; APO-1; APT1; Apo-1 Fas; CD95; FAS1; FASTM; TNFRSF6; |
Gi #:
N/A |
NCBI Acc #:
N/A |
Swiss Prot Acc #:
P25445 |
Length (aa): N/A |
Mol. Weight (Da): 37732 |
Chrom Location: N/A |
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 Clone: Alf2.1
Isotype: IgG1
Immunogen: Recombinant soluble active extracellular domain of human Fas ligand.
Antigen Species: Human
Specificity: Monoclonal Anti-Human Fas Ligand (CD95L) specifically recognizes the extracellular domain of human Fas ligand.
Target: FAS
Localization: Plasma membrane
Concentration: 1 mg/ml
Purification Note: The antibody is purified from culture supernatant of hybridoma cells, grown in a bioreactor.
Storage Buffer: Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4
Application Note: FACS: Use at an assay dependent dilution. A minimum working concentration of 1-2 µg/test is determined by flow cytometry, using cultured KFL9 cells (human chronic myelogenous leukemia cell line K562, stably-trasfected with human Fas ligand). When assayed by flow cytometric analysis, using the antibody at working concentration to stain 1 x 10^6 to 2 x 10^6 cells/0.1ml/test, fluorescence intensity is observed similar to that obtained with saturating antibody levels. The percentage population positive is also at the maximum percentage positive, using saturating antibody levels. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Cellular Localization: Plasma membrane
Source/Host: Mouse
Purity/Purification: Protein A purified
Clonality: Monoclonal
Crossreactivity: Human - Not yet tested in other species
Shipping: Products may be shipped on ice pack or dry ice. |
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| APPLICATIONS for FAS ANTIBODY: |
| FACS: Tested |
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| FAS ANTIBODY TARGET DESCRIPTION: |
Synonym Names for FAS antibody: FAS; 355; FAS; 134637; P25445; ALPS1A; APO-1; APT1; Apo-1 Fas; CD95; FAS1; FASTM; TNFRSF6;; Mouse monoclonal [Alf2.1] to Fas Ligand; 355; FAS; 134637; P25445; ALPS1A; APO-1; APT1; Apo-1 Fas; CD95; FAS1; FASTM; TNFRSF6;; Tumor necrosis factor receptor superfamily member 6
Homeostasis of multicellular organisms is controlled not only by the proliferation and differentiation of cells but also by cell death. The death of cells during embryogenesis, endocrine-dependent tissue atrophy, metamorphosis, a variety of pathologic conditions, and normal tissue turnover, is called programmed cell death (PCD). Most PCD proceeds by apoptosis, a process that includes condensation and segmentation of nuclei, condensation and fragmentation of the cytoplasm, and often extensive fragmentation of chromosomal DNA into nucleosome units. Many cells can be activated to undergo apoptosis following the interaction of selected ligands with cell surface receptors. The most well-studied receptors are CD95/Fas/Apo1 (apoptosis inducing protein 1) and tumor necrosis factor receptor 1 (TNFR1). Apoptosis mediated by either of these receptors results in activation of a family of cysteine proteases known as caspases. However, Fas-mediated death occurs much more rapidly than that triggered by the TNFR1. The action of Fas is mediated via FADD (Fas- associated death domain)/MORT1, an adapter protein that has a death domain at its C-terminus and binds to the cytoplasmic death domain of Fas. Human CD95/Fas/Apo-1 antigen is a single transmembrane glycoprotein receptor (325 amino acids, 45-48 kDa). An integral membrane protein, has been identified as the Fas ligand (Fas ligand, FasL, CD95L). FasL is a type II transmembrane glycoprotein and is a member of the TNF family, which includes TNFalpha, alpha- and beta-chains of lymphotoxin (LT), CD40 ligand and CD30 ligand. The amino acid sequences of human and murine FasL are 76.9% identical, and they are not species-specific. Membrane bound FasL (mFasL) is a 40 kDa protein, while the active soluble form of FasL (sFasL) was identified as a 26 kDa protein in the supernatant of activated peripheral T cells and cultured cells transfected with the full-length FasL DNA. FasL has four potential N-glycosylation sites which appear to be variably used. Consequently, the apparent MW of FasL may vary, per glycosylation and breakdown patterns in certain preparations. Engagement of Fas by its ligand results in the rapid induction of PCD in susceptible cells. This process bypasses the usual long sequence of signaling enzymes and immediately activates preexisting caspases. FasL is expressed on activated T cells whereas Fas is expressed on various types of cells. The cellular pathways that control apoptosis are critical to the maturation, selection, and survival of lymphocytes. Apoptosis or cell suicide is the physiological mode of lymphoid cell death in circumstances like negative selection of T cells in the thymus, ligation of CD4 and CD3 in mature T cells, downregulation of the immune response, clonal deletion of B cells by antigen, death of killer cell targets, cytokine-mediated killing, and tumor regression. Fas is also expressed on a number of lymphoma cell lines, on Epstein-Barr virus-transformed B lymphoblasts, and on a proportion of activated B and T cells. The production of excess soluble Fas protein would prevent cells from undergoing Fas ligand induced apoptosis and thereby permit tumor cells to escape immunosurveillance. The activation of mature T cells with phorbol myristic acetate (PMA) and ionomycin, concanavalin A (Con A) or anti-CD3, induces FasL gene expression. Herpes Simplex virus type 2 (HSV-2) but not HSV-1, potentially inhibits FasL surface expresssion in infected cells and thereby suppresses FasL-mediated cell death. Antibodies reacting specifically with Fas ligand are useful tools in the study of the intracellular pathways leading from membrane receptor engagement to apoptotic cell death, the tissue distribution and developmental expression pattern of Fas ligand, and its essential role during mammalian development especially in immune system homeostasis.
Function: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
Subunit: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B.
Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein.
Subcellular Location: Isoform 2: Secreted.
Subcellular Location: Isoform 3: Secreted.
Subcellular Location: Isoform 4: Secreted.
Subcellular Location: Isoform 5: Secreted.
Subcellular Location: Isoform 6: Secreted.
Tissue Specificity: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
Domain: Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
Disease: Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
Similarity: Contains 1 death domain.
Similarity: Contains 3 TNFR-Cys repeats.
FAS [Alf2.1] reacts with human - not yet tested in other species.
OMIM: 134637; gene. [NCBI / EBI]
601859; phenotype. [NCBI / EBI]
Products similar to FAS antibody:
IgG
 FAS [DX2], IgG
MMP15, IgG
CD95 [SA8 ], IgG
FAS (ready-to-use), IgG
FRA2, IgG
Protein
CD95 Human, Protein
Fas-activated serine/threonine kinase, Protein
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| PRODUCT INTERACTIONS: |
FAS [Alf2.1] interacts with Calmodulin Human, Protein (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Calmodulin (CaM) (C-term), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Caspase-8, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Caspase-8, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Caspase 8 [C502S], IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Rabbit anti Caspase 8 (Middle), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Rabbit anti Caspase 8 (CT), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Caspase 8 (ready-to-use), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Caspase 8, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Caspase 8, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Caspase 8, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Caspase-8 (N-term), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Caspase-8, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with DAXX, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with DAXX, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with DAXX [DAXX-01], IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with DAXX, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with DAXX, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with FADD (Fas (TNFRSF6)-associated via death domain), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with FADD, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with FADD [FD19], IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Recombinant Human Fas-Associated Death Domain, Protein (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with ASK1, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with ASK1 (Phospho-Ser83), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with ASK1 (Phospho-Ser966), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with ASK1 (Ab-83), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with ASK1 (Ab-966), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with ASK1 (Phospho-Ser83) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with ASK1 (Phospho-Ser966) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Rabbit anti ASK-1 pS83, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Anti-ASK-1 phospho specific pS83, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with MEKK5 / ASK1 (N-term), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Tyrosine-protein phosphatase non-receptor type 13, Protein (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with PTPN13, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with SRC, Protein (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with SRC, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with SRC, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Src (Phospho-Tyr418), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Src (Phospho-Tyr529), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Src (Ab-418), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Src (Ab-529), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Src(Phospho-Tyr418) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Src (Phospho-Tyr529) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Src Mouse, Protein (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with SRC, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with SRC phospho Y418, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Src (pY529), IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Src, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with Src, IgG (GenWay Catalog #: 20-272-190231).
FAS [Alf2.1] interacts with YES1 Human, Protein (GenWay Catalog #: 20-272-190231).
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SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: FAS antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of FAS antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about FAS antibody, all related information, articles, resources about FAS antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
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