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| S100 beta [SH-B1] Antibody, Mouse Monoclonal Antibody |
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| Catalog Number: 20-272-190390 |
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| Related Product Names:
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S100 beta antibody; S100 beta; 282059; S100B;; S100 beta [SH-B1]
- Mouse monoclonal [SH - B1] to S100 beta; 282059; S100B;; Protein S100-B |
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- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
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| Gene Name: S100 beta |
Gene Name Synonym: 282059; S100B; |
Gi #:
N/A |
NCBI Acc #:
NP_006263.1 |
Swiss Prot Acc #:
P04271 |
Length (aa): N/A |
Mol. Weight (Da): 10713 |
Chrom Location: N/A |
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 Clone: SH-B1
Isotype: IgG1
Immunogen: Full length native protein (purified) (Cow).
Antigen Species: Bovine
Specificity: The product does not react with other members of the EF-hand family such as calmodulin, parvalbumin, intestinal calcium binding protein, and myosin light chain.
Positive Control: Human tongue tissue slide (IHC-P)
Target: S100 beta
Concentration: ascites
Storage Buffer: Preservative: 15mM Sodium Azide
Application Note: Dot: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/1000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Use at an assay dependent dilution. Predicted molecular weight: 10.5 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Cellular Localization: Cytoplasmic
Source/Host: Mouse
Purity/Purification: Unpurified
Clonality: Monoclonal
Crossreactivity: Cross-reacts with Human, Rat, Cow, Cat, Pig and Rabbit. Not yet tested in other species.
Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.
Shipping: Products may be shipped on ice pack or dry ice. |
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| APPLICATIONS for S100 BETA ANTIBODY: |
| Dot, ELISA, IHC-P, WB: Tested |
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| S100 BETA ANTIBODY TARGET DESCRIPTION: |
Synonym Names for S100 beta antibody: S100 beta; 282059; S100B;; Mouse monoclonal [SH - B1] to S100 beta; 282059; S100B;; Protein S100-B
S100 is a set of small, thermolabile, highly acidic dimer proteins of approximately 20 kDa which are widely distributed in different tissues. Dimeric combinations of two chains, the alpha chain (93 a.a., 10.4 kDa) and the beta chain (91 a.a. 10.5 kDa), form the three known subtypes of S100: S100ao (alpha alpha), S100a (alpha beta) and S100b (beta beta). The S100 molecule is markedly conserved in the amino acid sequence although there is a slight variation of the primary structure in different species. The protein extracted from different organs of the same species is identical. The alpha and beta chains are 58% homologous (54 a.a.). Both have divalent cation binding sites situated toward the carboxy terminus and apparently have similar functional features. S100 can be grouped with other calcium binding proteins such as calmodulin, parvalbumin, intestinal calcium binding protein, myosin light chain and troponin-C. It shows a significant sequence homology with these proteins, particularly around the calcium binding domain. Hence, S100 is a calcium modulated protein2 that binds calcium and zinc ions reversibly at physiologic pH and ionic strength, followed by a conformational change in the molecule. S100 is considered to be a cell growth regulator, but other functions have been suggested, e.g., increasing the membrane permeability to cations under physiologic conditions, stimulation of nucleolar RNA polymerase activity and as a carrier of proteins and free fatty acids in adipocytes. Human S100 containing cells are subdivided to three groups: S100b containing cells, such as Schwann cells, pituicytes of the neurohypophysis, Langerhansí cells and interdigitating cells; S100a containing cells such as glial cells and melanocytes; and S100ao containing cells such as neurons, ganglion cells, slow muscle cells, cardiac cells, monocytes and some macrophages. Although the tissue distribution of S100 is known to be too broad to conform to a single histologic pattern, it is nonetheless sufficiently restricted that the localization of this protein is useful in the differential diagnosis of neoplasms and proliferative processes. Monoclonal antibody reacting specifically against the beta subunit of S100 is a useful tool in distinguishing malignant melanoma from undifferentiated carcinoma or lymphoma, and in distinguishing gliamyomas and schwannomas and their counterparts in the gastrointestinal tract.
Function: Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase.
Subunit: Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38 (By similarity). The S100B dimer interacts with two molecules of CAPZA1.
Subcellular Location: Cytoplasm. Nucleus.
Tissue Specificity: Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
Miscellaneous: In addition to metal-ion binding, this protein is involved with the regulation of protein phosphorylation in brain tissue.
Similarity: Belongs to the S-101 family.
Similarity: Contains 2 EF-hand domains.
S100 beta [SH-B1] reacts with cross-reacts with human, rat, cow, cat, pig and rabbit. not yet tested in other species..
OMIM: 176990; gene. [NCBI / EBI]
Products similar to S100 beta antibody:
IgG
 S100 protein, IgG
S100, IgG
S100 [4C4.9], IgG
S100 protein, IgG
S101 [6G1], IgG
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| PRODUCT INTERACTIONS: |
S100 beta [SH-B1] interacts with S100A1 Human, Protein (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100 [4B3], IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100 (ready-to-use), IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100 alpha, IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100 [4C4.9], IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100 [8B10], IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100 [3B10], IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100, IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S-100 (alpha-beta), IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with Sheep A' S-100A1 Pig/Bovine, IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with Rabbit anti S-100, IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with Human Brain S-100 Protein, Native_Protein (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100 alpha [SH-A1], IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S101 [6G1], IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100 (ready-to-use), IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100, IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100A11 Human, Protein (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100A11, IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100A11, IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100A11, IgG (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100A11: Human, Recombinant, E Coli, Protein (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100A11: Human, Recombinant, E Coli, Protein (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100A11: Human, Recombinant, E Coli, Protein (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100A11: Human, Recombinant, E Coli, Protein (GenWay Catalog #: 20-272-190390).
S100 beta [SH-B1] interacts with S100A6, Native_Protein (GenWay Catalog #: 20-272-190390).
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SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: S100 beta antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of S100 beta antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about S100 beta antibody, all related information, articles, resources about S100 beta antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
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