Glucocorticoid receptor antibody, Glucocorticoid receptor [8E9] Antibody, Mouse Monoclonal Antibody

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Glucocorticoid receptor [8E9] Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-272-190483

Buy Glucocorticoid receptor antibody -

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Related Product Names:

- Glucocorticoid receptor antibody; Glucocorticoid receptor; 2908; NR3C1; 138040; P04150; GCCR; GCR; GR; GRL;; Glucocorticoid receptor [8E9]
- Mouse monoclonal [8E9] to Glucocorticoid Receptor; 2908; NR3C1; 138040; P04150; GCCR; GCR; GR; GRL;; Glucocorticoid receptor

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: Glucocorticoid receptor	Gene Name Synonym: 2908; NR3C1; 138040; P04150; GCCR; GCR; GR; GRL;
Gi #: N/A	NCBI Acc #: NP_000167.1	Swiss Prot Acc #: P04150
Length (aa): N/A	Mol. Weight (Da): 85659	Chrom Location: N/A

Clone: 8E9

Isotype: IgG1

Immunogen: 26 amino acid peptide corresponding to residues 150-176 of human GCR linked to thyroglobulin (human).

Antigen Species: Human

Specificity: GTX11379 recognises the human glucocorticoid receptor.

Target: Glucocorticoid receptor

Localization: Cytoplasmic in the absence of ligand; nuclear after ligand-binding.

Concentration: 1 mg/ml

Purification Note: Purified IgG prepared by affinity chromatography from tissue culture supernatant.

Storage Buffer: Preservative: 0.09% Sodium Azide; Constituents: 0.1% BSA, PBS. pH 7.2

Application Note: ELISA: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Predicted molecular weight: 93.24 kDa. Is unsuitable for IHC-P, IHC-Fr or IHC-R. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular Localization: Cytoplasmic in the absence of ligand; nuclear after ligand-binding.

Myeloma: Sp2/0-Ag14

Source/Host: Mouse

Purity/Purification: Protein G purified

Clonality: Monoclonal

Crossreactivity: Human - Not yet tested in other species

Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for GLUCOCORTICOID RECEPTOR ANTIBODY:

ELISA, WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

GLUCOCORTICOID RECEPTOR ANTIBODY TARGET DESCRIPTION:

Synonym Names for Glucocorticoid receptor antibody: Glucocorticoid receptor; 2908; NR3C1; 138040; P04150; GCCR; GCR; GR; GRL;; Mouse monoclonal [8E9] to Glucocorticoid Receptor; 2908; NR3C1; 138040; P04150; GCCR; GCR; GR; GRL;; Glucocorticoid receptor

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.

Function: Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation (By similarity).

Subunit: Heteromultimeric cytoplasmic complex with HSP90, HSP70, and FKBP5 or another immunophilin, or the immunophilin homolog PPP5C. Directly interacts with UNC45A. Upon ligand binding FKBP5 dissociates from the complex and FKBP4 takes its place, thereby linking the complex to dynein and mediating transport to the nucleus where the complex dissociates (By similarity). Binds to DNA as a homodimer, and as a heterodimer with NR3C2 or the retinoid X receptor. Binds STAT5A and STAT5B homodimers and heterodimers. Interacts with NRIP1, POU2F1, POU2F2 and TRIM28. Interacts with NCOA1, NCOA3, SMARCA4, SMARCC1, SMARCD1, and SMARCE1 (By similarity). Interacts with several coactivator complexes, including the SMARCA4 complex, CREBBP/EP300, TADA2L and p160 coactivators such as NCOA2 and NCOA6. Interaction with BAG1 inhibits transactivation. Interacts with HEXIM1, PELP1 and TGFB1I1.

Subcellular Location: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand, nuclear after ligand-binding.

Tissue Specificity: Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart.

Domain: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.

Ptm: Increased proteasome-mediated degradation in response to glucocorticoids.

Ptm: Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.

Ptm: Sumoylated; this reduces transcription transactivation.

Ptm: Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling (By similarity).

Polymorphism: Carriers of the 22-Glu-Lys-23 allele are relatively more resistant to the effects of GCs with respect to the sensitivity of the adrenal feedback mechanism than non-carriers, resulting in a better metabolic health profile. Carriers have a better survival than non-carriers, as well as lower serum CRP levels. The 22-Glu-Lys-23 polymorphism is associated with a sex-specific, beneficial body composition at young-adult age, as well as greater muscle strength in males.

Disease: Defects in NR3C1 are a cause of glucocorticoid resistance [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant.

Miscellaneous: High constitutive expression of isoform beta by neutrophils may provide a mechanism by which these cells escape glucocorticoid-induced cell death. Up-regulation by proinflammatory cytokines such as IL8 further enhances their survival in the presence of glucocorticoids during inflammation.

Miscellaneous: Can up- or down-modulate aggregation and nuclear localization of expanded polyglutamine polypeptides derived from AR and HD through specific regulation of gene expression. Aggregation and nuclear localization of expanded polyglutamine proteins are regulated cellular processes that can be modulated by this receptor, a well-characterized transcriptional regulator.

Similarity: Belongs to the nuclear hormone receptor family. NR3 subfamily.

Similarity: Contains 1 nuclear receptor DNA-binding domain.

Glucocorticoid receptor [8E9] reacts with human - not yet tested in other species.

OMIM: 138040; gene+phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Neuroactive ligand-receptor interaction 04080

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PRODUCT INTERACTIONS:

Glucocorticoid receptor [8E9] interacts with SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), IgG (GenWay Catalog #: 20-272-190483).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

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Precautions: Glucocorticoid receptor antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of Glucocorticoid receptor antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about Glucocorticoid receptor antibody, all related information, articles, resources about Glucocorticoid receptor antibody are being stored on our online database. Let us know if you have questions regarding this product.

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