E Cadherin antibody, E Cadherin [DECMA-1] Antibody, Rat Monoclonal Antibody

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E Cadherin [DECMA-1] Antibody, Rat Monoclonal Antibody

Catalog Number: 20-272-190575

Buy E Cadherin antibody -

Volume:

Related Product Names:

- E Cadherin antibody; E Cadherin; 12550; Cdh1; AA960649; E-cadherin; Ecad; MGC107495; UVO; Um; uvomorulin; 999; CDH1; 137215; P12830; Arc-1; CDHE; ECAD; LCAM; UVO; E Cadherin [DECMA-1]
- Rat monoclonal [DECMA - 1] to E Cadherin; 12550; Cdh1; AA960649; E-cadherin; Ecad; MGC107495; UVO; Um; uvomorulin; 999; CDH1; 137215; P12830; Arc-1; CDHE; ECAD; LCAM; UVO;; Epithelial cadherin

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: E Cadherin	Gene Name Synonym: 12550; Cdh1; AA960649; E-cadherin; Ecad; MGC107495; UVO; Um; uvomorulin; 999; CDH1; 137215; P12830; Arc-1; CDHE; ECAD; LCAM; UVO
Gi #: N/A	NCBI Acc #: NP_004351.1	Swiss Prot Acc #: P12830
Length (aa): N/A	Mol. Weight (Da): 97456	Chrom Location: N/A

Clone: DECMA-1

Isotype: IgG1

Immunogen: Mouse embryonal carcinoma cell line PCC4 Aza RI.

Antigen Species: Mouse

Positive Control: Tested with MDCK cells

Target: E Cadherin

Concentration: ascites

Storage Buffer: Preservative: 15mM Sodium Azide; Constituents: Raw Ascites

Application Note: IF: Use at a dilution of 1/1600. IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 106 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Source/Host: Rat

Purity/Purification: Ascites

Clonality: Monoclonal

Crossreactivity: Cross-reacts with Mouse and Dog. Not yet tested in other species.

Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for E CADHERIN ANTIBODY:

IF, IHC-Fr, IP, WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

E CADHERIN ANTIBODY TARGET DESCRIPTION:

Synonym Names for E Cadherin antibody: E Cadherin; 12550; Cdh1; AA960649; E-cadherin; Ecad; MGC107495; UVO; Um; uvomorulin; 999; CDH1; 137215; P12830; Arc-1; CDHE; ECAD; LCAM; UVO; Rat monoclonal [DECMA - 1] to E Cadherin; 12550; Cdh1; AA960649; E-cadherin; Ecad; MGC107495; UVO; Um; uvomorulin; 999; CDH1; 137215; P12830; Arc-1; CDHE; ECAD; LCAM; UVO;; Epithelial cadherin

Cell-cell interactions during embryonic development and in tissue organization involve specific cell-adhesion molecules (CAMs) that are functionally defined by antibodies that interfere with cell-cell adhesion. CAMs are expressed on early embryonic cells and persist in derivatives of all three germ layers. The best characterized CAMs are N-CAM (neural CAM) and L-CAM (liver CAM). The protein Uvomorulin, initially identified in embryonal carcinoma, is identical to L-CAM, E-Cadherin, Cell CAM 80/120 and ARC-1, each of which have been characterized in different systems. E-Cadherin has been characterized as a 120 kDa cell surface glycoprotein from which an 84 kDa fragment can be released by trypsin digestion in the presence of Ca²⁺.

Function: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.

Function: E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

Subunit: Homodimer; disulfide-linked. Interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. Interaction with PSEN1, cleaves CDH1 resulting in the disassociation of cadherin-based adherens junctions (CAJs). Interacts with AJAP1, CTNND1 and DLGAP5.

Subcellular Location: Cell junction. Cell membrane; Single-pass type I membrane protein. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma-catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm.

Tissue Specificity: Non-neural epithelial tissues.

Ptm: During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3 to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disaaaembly of adherens junctions.

Disease: Defects in CDH1 are involved in dysfunction of the cell-cell adhesion system, triggering cancer invasion (gastric, breast, ovary, endometrium and thyroid) and metastasis.

Disease: Defects in CDH1 are a cause of hereditary diffuse gastric cancer (HDGC) [MIM:137215].

Disease: Defects in CDH1 are a cause of susceptibility to endometrial cancer [MIM:608089].

Similarity: Contains 5 cadherin domains.

E Cadherin [DECMA-1] reacts with cross-reacts with mouse and dog. not yet tested in other species..

OMIM: 137215; phenotype. [NCBI / EBI]
192090; gene. [NCBI / EBI]
608089; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Adherens junction 04520
KEGG pathway: Cell adhesion molecules (CAMs) 04514
KEGG pathway: Endometrial cancer 05213
KEGG pathway: Melanoma 05218
KEGG pathway: Pathogenic Escherichia coli infection - EHEC 05130
KEGG pathway: Pathogenic Escherichia coli infection - EPEC 05131
KEGG pathway: Thyroid cancer 05216
Reactome Event:Apoptosis 109581
Reactome Event:Signaling in Immune system 168256

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PRODUCT INTERACTIONS:

E Cadherin [DECMA-1] interacts with CTNNB1, IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with CATENIN, IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with b-Catenin (CTNNB1) Dephospho, IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with b-Catenin (CTNNB1), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with BETA-CATENIN, IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta Catenin [6F9], IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta Catenin [15B8], IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta-Catenin (Phospho-Thr41/Ser45), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with b-Catenin (Ab-41/45), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta-Catenin (Phospho-Ser33), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta-Catenin (Phospho-Ser37), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with Catenin (Ab-33), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with Catenin (Ab-37), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta-Catenin (Phospho-Thr41/Ser45) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta-Catenin (Phospho-Ser33) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta-Catenin (Phospho-Ser37) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with Catenin beta, C-terminal (exon 14), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with Catenin beta, N-terminal (exon 2), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with Catenin, beta, Core, IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with Catenin beta, C-terminal (a.a. 769-781), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with Catenin, beta, dephosphorylated (35-50), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with Catenin beta, pTyr 86, IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with Catenin beta, pTyr 654, IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with Catenin, beta, dephosphorylated (27-37), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with Catenin beta,(exon 3), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta Catenin phospho S33, S37 [BC-22], IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta Catenin [15B8] (Cy3), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta Catenin (ready-to-use), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta Catenin, IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with beta Catenin phospho S33 [BC-76], IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with FGFR1, IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with FGFR1 [M19B2], IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with FGFR1 [M5G10], IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with FGFR1 [M17D10], IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with FGFR1 alpha [M2F12], IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with FGFR1 alpha [M1C4], IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with FGFR1 beta [M17A3], IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with FGF Receptor 1 alpha, IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with FGF Receptor 1 beta, IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with FGFR1 Human, Protein (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with Serine/threonine-protein kinase D1, Protein (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with PKD/PKC-mu (Phospho-Ser738), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with PKD/PKC-mu (Phospho-Ser910), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with PKD/PKC-mu (Ab-738), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with PKD/PKC-mu (Ab-910), IgG (GenWay Catalog #: 20-272-190575).

E Cadherin [DECMA-1] interacts with PKC mu, IgG (GenWay Catalog #: 20-272-190575).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: E Cadherin antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of E Cadherin antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about E Cadherin antibody, all related information, articles, resources about E Cadherin antibody are being stored on our online database. Let us know if you have questions regarding this product.

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