MiTF antibody, MiTF [C5] Antibody, Mouse Monoclonal Antibody

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MiTF [C5] Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-272-190706

Buy MiTF antibody -

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Related Product Names:

- MiTF antibody; MiTF; Microphthalmia Associated Transcription Factor MITF WS2A; MiTF [C5]
- Mouse monoclonal [C5] to microphthalmia; Microphthalmia Associated Transcription Factor MITF WS2A; Microphthalmia-associated transcription factor

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: MiTF	Gene Name Synonym: Microphthalmia Associated Transcription Factor MITF WS2A
Gi #: N/A	NCBI Acc #: NP_000239.1	Swiss Prot Acc #: O75030
Length (aa): N/A	Mol. Weight (Da): 58795	Chrom Location: N/A

Clone: C5

Isotype: IgG1

Immunogen: N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.

Antigen Species: Human

Positive Control: 501 Mel human melanoma cells and wild-type human, rat or mouse osteoclast cells

Target: MiTF

Concentration: 1 mg/ml

Storage Buffer: Phosphate-buffered saline containing 0.08% sodium azide

Application Note: For IP: Use at a concentration of 2 ug/ml. For WB: Use at a concentration of 1 ug/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. The usefulness of this product in other applications has not been determined.

Source/Host: Mouse

Purity/Purification: Protein G purified

Clonality: Monoclonal

Crossreactivity: Human, Mouse, and Rat - Not yet tested in other species

Format: Liquid

Storage: Upon Receipt - Keep as concentrated solution. Aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for MITF ANTIBODY:

GSA, IHC-Fr, IHC-P, IP, WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

MITF ANTIBODY TARGET DESCRIPTION:

Synonym Names for MiTF antibody: MiTF; Microphthalmia Associated Transcription Factor MITF WS2A; Mouse monoclonal [C5] to microphthalmia; Microphthalmia Associated Transcription Factor MITF WS2A; Microphthalmia-associated transcription factor

This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome.

Function: Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Subunit: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA in the form of homodimer or heterodimer with either TFE3, TFEB or TFEC.

Subcellular Location: Nucleus.

Tissue Specificity: Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.

Ptm: Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.

Disease: Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.

Disease: Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.

Disease: Defects in MITF are the cause of Tietz syndrome [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.

Similarity: Belongs to the MiT/TFE family.

Similarity: Contains 1 basic helix-loop-helix (bHLH) domain.

MiTF [C5] reacts with human, mouse, and rat - not yet tested in other species .

OMIM: 103470; phenotype. [NCBI / EBI]
103500; phenotype. [NCBI / EBI]
156845; gene. [NCBI / EBI]
193510; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Melanogenesis 04916
KEGG pathway: Melanoma 05218

Products similar to MiTF antibody:

IgG

MITF (microphthalmia-associated transcription factor), IgG

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: MiTF antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of MiTF antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about MiTF antibody, all related information, articles, resources about MiTF antibody are being stored on our online database. Let us know if you have questions regarding this product.

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