Melanoma antibody, Melanoma [HMB45 + DT101 + BC199] Antibody, Mouse Monoclonal Antibody

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Melanoma [HMB45 + DT101 + BC199] Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-272-192073

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Related Product Names:

- Melanoma antibody; Melanoma; CDKN2; MLM; Melanoma [HMB45 + DT101 + BC199]
- Mouse monoclonal [HMB45 + DT101 + BC199] to Melanoma; Cyclin-dependent kinase inhibitor 2A, isoform 4

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: Melanoma	Gene Name Synonym: CDKN2; MLM
Gi #: N/A	NCBI Acc #: N/A	Swiss Prot Acc #: Q8N726
Length (aa): N/A	Mol. Weight (Da): 18006	Chrom Location: N/A

Clone: HMB45 + DT101 + BC199

Isotype: IgG

Light chain: kappa

Immunogen: HMB45 - Pigmented melanoma metastases from LN MART-1 - Recombinant human MART-1 protein

Antigen Species: Human

Specificity: The HMB-45 clone reacts with a neuraminidase-sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45-reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. This antibody has been shown to label the majority of melanomas. MART-1 recognizes a protein of 18kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 recognizes a subcellular fraction found in melanosomes. The antibody labels melanomas and tumors showing melanocytic differentiation. It does not mark neoplasms of epithelial origin, lymphomas or mesenchymal tumors. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART-1 is more sensitive than HMB-45 when labeling metastatic melanomas.

Positive Control: Metastatic melanoma in lymph node

Target: Melanoma

Localization: Cytoplasmic

Concentration: 0.056 mg/ml

Storage Buffer: Preservative: Proprietary, not sodium azide or thimerosal. Constituents: PBS, Tissue Culture Supernatant. pH 7.3; Protein carrier.

Application Note: IHC:For an ABC system, dilute 1:50-100 and incubate for 30-60 minutes at RT. For formalin fixed paraffin embedded sections, the staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail. It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment, therefore we recommend you substitute an AEC or a Fast Red substrate protocol

Cellular Localization: Cytoplasmic

Source/Host: Mouse

Purity/Purification: n/a

Clonality: Monoclonal

Crossreactivity: Cross-reacts with Human, Rat and Mouse. Not yet tested in other species.

Format: This antibody is produced in tissue culture supernatant, concentrated and then placed in to PBS pH7.3 with a protein carrier and a proprietary preservative that is not sodium azide or thimerosal.

Storage: Keep as concentrated solution, aliquot and store at 4C.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for MELANOMA ANTIBODY:

IHC-Fr, IHC-P: Tested

TESTING: (secondary reagents and protocols )

Not Available

MELANOMA ANTIBODY TARGET DESCRIPTION:

Synonym Names for Melanoma antibody: Melanoma; CDKN2; MLM; Mouse monoclonal [HMB45 + DT101 + BC199] to Melanoma; Cyclin-dependent kinase inhibitor 2A, isoform 4

HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones DT101 and BC199. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.

Function: Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development.

Subunit: Does not interact with cyclins, CDC2, CDK2, CDK4, CDK5 or CDK6. Binds to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI and UBE2I/UBC9. Interacts with TBRG1. Interacts with CDKN2AIP and E4F1.

Subcellular Location: Nucleus, nucleolus (By similarity).

Melanoma [HMB45 + DT101 + BC199] reacts with cross-reacts with human, rat and mouse. not yet tested in other species..

OMIM: 600160; gene. [NCBI / EBI]

Products similar to Melanoma antibody:

IgG

CDK4 (cyclin-dependent kinase 4), IgG

MAGE 1 (ready-to-use), IgG

Melan A (ready-to-use), IgG

INK4c, IgG

beta Actin [AC-15], IgG

Protein

CDKN2A Human, Protein

Melanoma Human, Protein

Cyclin-dependent kinase 6 inhibitor, Protein

PRODUCT INTERACTIONS:

Melanoma [HMB45 + DT101 + BC199] interacts with MDM2 [2A10], IgG (GenWay Catalog #: 20-272-192073).

Melanoma [HMB45 + DT101 + BC199] interacts with MDM2 [SMP14], IgG (GenWay Catalog #: 20-272-192073).

Melanoma [HMB45 + DT101 + BC199] interacts with Rabbit anti Mouse MDM2 pS185, IgG (GenWay Catalog #: 20-272-192073).

Melanoma [HMB45 + DT101 + BC199] interacts with MDM2 [HDM2-323], IgG (GenWay Catalog #: 20-272-192073).

Melanoma [HMB45 + DT101 + BC199] interacts with MDM2 [MD-219], IgG (GenWay Catalog #: 20-272-192073).

Melanoma [HMB45 + DT101 + BC199] interacts with MDM2 (ready-to-use), IgG (GenWay Catalog #: 20-272-192073).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

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Precautions: Melanoma antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of Melanoma antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about Melanoma antibody, all related information, articles, resources about Melanoma antibody are being stored on our online database. Let us know if you have questions regarding this product.

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