CD95 antibody, CD95 [LT95] Antibody, Mouse Monoclonal Antibody

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CD95 [LT95] Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-272-192193

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Related Product Names:

- CD95 antibody; CD95; APO 1, Apo 1 antigen, APO 1 cell surface antigen, Apoptosis Antigen 1, Apoptosis APO 1 antigen 1, Apoptosis mediating surface an; CD95 [LT95]
- Mouse monoclonal [LT95] to CD95 ; 355; FAS; 134637; P25445; ALPS1A; APO-1; APT1; Apo-1 Fas; CD95; FAS1; FASTM; TNFRSF6;; 355; FAS; 134637; P25445; ALPS1A; APO-1; APT1; Apo-1 Fas; CD95; FAS1; FASTM; TNFRSF6;

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: CD95	Gene Name Synonym: APO 1, Apo 1 antigen, APO 1 cell surface antigen, Apoptosis Antigen 1, Apoptosis APO 1 antigen 1, Apoptosis mediating surface an
Gi #: N/A	NCBI Acc #: N/A	Swiss Prot Acc #: P25445
Length (aa): N/A	Mol. Weight (Da): 37732	Chrom Location: N/A

Clone: LT95

Isotype: IgG1

Immunogen: Tissue / cell preparation (Human). (T cell lymphoma cells HUT-78).

Antigen Species: Human

Specificity: Human Fas (CD95/APO-1).

Positive Control: Reacts strongly with IM9 and HUT-78 cell lines. Reacts moderately with Raji, Ramos, U266, Jurkat, BJAB and ML-1 cell lines. Reacts weakly with KG1a, U937, KM3 and TF-1 cell lines. Does not react with K562 and THP-1 cell lines.

Target: CD95

Localization: Type I membrane protein (isoform 1); Secreted (isoforms 2 to 6).

Concentration: 1 mg/ml

Purification Note: Purified from ascites by DEAE-chromatography and precipitation methods.

Storage Buffer: Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Application Note: FACS: Use at an assay dependant concentration. Does not induce Fas mediated apoptosis in susceptible cells. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular Localization: Type I membrane protein (isoform 1); Secreted (isoforms 2 to 6).

Source/Host: Mouse

Purity/Purification: >95% by SDS-PAGE

Clonality: Monoclonal

Crossreactivity: Human - Not yet tested in other species

Format: Liquid

Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for CD95 ANTIBODY:

FACS: Tested

TESTING: (secondary reagents and protocols )

Not Available

CD95 ANTIBODY TARGET DESCRIPTION:

Synonym Names for CD95 antibody: CD95; APO 1, Apo 1 antigen, APO 1 cell surface antigen, Apoptosis Antigen 1, Apoptosis APO 1 antigen 1, Apoptosis mediating surface an; Mouse monoclonal [LT95] to CD95 ; 355; FAS; 134637; P25445; ALPS1A; APO-1; APT1; Apo-1 Fas; CD95; FAS1; FASTM; TNFRSF6;; 355; FAS; 134637; P25445; ALPS1A; APO-1; APT1; Apo-1 Fas; CD95; FAS1; FASTM; TNFRSF6;

CD95, also known as FAS or APO-1, is a 36 kDa cell surface type I- membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. CD95 is a member of the TNF receptor family, which includes TNFR-1, TNFR-2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti-CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl-terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase-8 that together with the receptor form the death-inducing signaling complex (DISC).

Function: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

Subunit: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BRE and FEM1B.

Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein.

Subcellular Location: Isoform 2: Secreted.

Subcellular Location: Isoform 3: Secreted.

Subcellular Location: Isoform 4: Secreted.

Subcellular Location: Isoform 5: Secreted.

Subcellular Location: Isoform 6: Secreted.

Tissue Specificity: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.

Domain: Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.

Disease: Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.

Similarity: Contains 1 death domain.

Similarity: Contains 3 TNFR-Cys repeats.

CD95 [LT95] reacts with human - not yet tested in other species.

OMIM: 134637; gene. [NCBI / EBI]
601859; phenotype. [NCBI / EBI]

Products similar to CD95 antibody:

IgG

FAS [5F-7], IgG

FAS [H11] (Biotin), IgG

FAS [101624], IgG

FAS [Alf2.1], IgG

PTPN13, IgG

PRODUCT INTERACTIONS:

CD95 [LT95] interacts with Calmodulin Human, Protein (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Caspase-8, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Caspase 8 [C502S], IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Rabbit anti Caspase 8 (Middle), IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Rabbit anti Caspase 8 (CT), IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Caspase 8 (ready-to-use), IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Caspase 8, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with DAXX, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with DAXX [DAXX-01], IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with DAXX, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with FADD (Fas (TNFRSF6)-associated via death domain), IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with FADD, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with FADD [FD19], IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with ASK1, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with ASK1 (Phospho-Ser83), IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with ASK1 (Phospho-Ser966), IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with ASK1 (Ab-83), IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with ASK1 (Ab-966), IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with ASK1 (Phospho-Ser83) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with ASK1 (Phospho-Ser966) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Rabbit anti ASK-1 pS83, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Anti-ASK-1 phospho specific pS83, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Tyrosine-protein phosphatase non-receptor type 13, Protein (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with PTPN13, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with SRC, Protein (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with SRC, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with SRC phospho Y529, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Src (Phospho-Tyr418), IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Src (Phospho-Tyr529), IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Src (Ab-418), IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Src (Ab-529), IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Src(Phospho-Tyr418) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Src (Phospho-Tyr529) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with Src Mouse, Protein (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with SRC, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with SRC phospho Y418, IgG (GenWay Catalog #: 20-272-192193).

CD95 [LT95] interacts with YES1 Human, Protein (GenWay Catalog #: 20-272-192193).

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Precautions: CD95 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of CD95 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about CD95 antibody, all related information, articles, resources about CD95 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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