Caspase 3 antibody, Caspase 3 [T46L] Antibody, Mouse Monoclonal Antibody

Alphabetical Listing of Primary Antibodies:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Alphabetical Listing of Proteins:	f\| 0-4 \| 5-9 \| A \| B \| C \| D \| E \| F \| G \| H \| I \| J \| K \| L \| M \| N \| O \| P \| Q \| R \| S \| T \| U \| V \| W \| X \| Y \| Z \|
Peptides \| Secondary Antibodies \| ELISA Kits

Caspase 3 [T46L] Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-272-192361

Buy Caspase 3 antibody -

Size:

Related Product Names:

- Caspase 3 antibody; Caspase 3; APOPAIN, Apopain precursor, CASP3, Caspase 3 apoptosis related cysteine protease, CPP 32, CPP32, CPP32B, Cysteine protease CPP32; Caspase 3 [T46L]
- Mouse monoclonal [T46L] to Caspase 3; 12367; Casp3; A830040C14Rik; Apopain; CPP32; Caspase-3; Lice; Yama;; 12367; Casp3; A830040C14Rik; Apopain; CPP32; Caspase-3; Lice; Yama;

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: Caspase 3	Gene Name Synonym: APOPAIN, Apopain precursor, CASP3, Caspase 3 apoptosis related cysteine protease, CPP 32, CPP32, CPP32B, Cysteine protease CPP32
Gi #: N/A	NCBI Acc #: NP_004337.2	Swiss Prot Acc #: P42574
Length (aa): N/A	Mol. Weight (Da): 31608	Chrom Location: N/A

Clone: T46L

Isotype: IgG1

Immunogen: Full length protein (Mouse).

Antigen Species: Mouse

Specificity: This antibody recognizes 32 kDa Caspase 3.

Target: Caspase 3

Concentration: 0.2 mg/ml

Storage Buffer: PBS with 1 mg/ml BSA, 1.5 mM sodium azide and 50% glycerol

Application Note: WB: Use at a concentration of 1 - 2 µg/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Source/Host: Mouse

Purity/Purification: n/a

Clonality: Monoclonal

Crossreactivity: Cross-reacts with Mouse and Rat. Not yet tested in other species.

Format: Liquid (250µl)

Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for CASPASE 3 ANTIBODY:

WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

CASPASE 3 ANTIBODY TARGET DESCRIPTION:

Synonym Names for Caspase 3 antibody: Caspase 3; APOPAIN, Apopain precursor, CASP3, Caspase 3 apoptosis related cysteine protease, CPP 32, CPP32, CPP32B, Cysteine protease CPP32; Mouse monoclonal [T46L] to Caspase 3; 12367; Casp3; A830040C14Rik; Apopain; CPP32; Caspase-3; Lice; Yama;; 12367; Casp3; A830040C14Rik; Apopain; CPP32; Caspase-3; Lice; Yama;

The caspase family of cysteine proteases plays a key role in apoptosis. Caspase 3 is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase 3 consists of large (17 kD) and small (12 kD) subunits that associate to form an active enzyme. The active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).

Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.

Catalytic Activity: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position.

Enzyme Regulation: Inhibited by isatin sulfonamides.

Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.

Subcellular Location: Cytoplasm.

Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Ptm: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.

Ptm: S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.

Similarity: Belongs to the peptidase C14A family [view classification].

Caspase 3 [T46L] reacts with cross-reacts with mouse and rat. not yet tested in other species..

OMIM: 600636; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Alzheimer's disease 05010
KEGG pathway: Apoptosis 04210
KEGG pathway: Colorectal cancer 05210
KEGG pathway: Dentatorubropallidoluysian atrophy (DRPLA) 05050
KEGG pathway: Epithelial cell signaling in Helicobacter pylori infection 05120
KEGG pathway: Huntington's disease 05040
KEGG pathway: MAPK signaling pathway 04010
KEGG pathway: Natural killer cell mediated cytotoxicity 04650
KEGG pathway: Neurodegenerative Disorders 01510
Reactome Event:Apoptosis 109581
Reactome Event:Signalling by NGF 166520

Products similar to Caspase 3 antibody:

IgG

Caspase 3 protease, IgG

Caspase 3 [3F48], IgG

Caspase 3 active, IgG

Caspase 3, IgG

Caspase 3 [3F49], IgG

PRODUCT INTERACTIONS:

Caspase 3 [T46L] interacts with Baculoviral IAP repeat-containing protein 2, IgG (GenWay Catalog #: 20-272-192361).

Caspase 3 [T46L] interacts with cIAP1, IgG (GenWay Catalog #: 20-272-192361).

Caspase 3 [T46L] interacts with Baculoviral IAP repeat-containing protein 7, IgG (GenWay Catalog #: 20-272-192361).

Caspase 3 [T46L] interacts with LIVIN, IgG (GenWay Catalog #: 20-272-192361).

Caspase 3 [T46L] interacts with Livin [88C570], IgG (GenWay Catalog #: 20-272-192361).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: Caspase 3 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of Caspase 3 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about Caspase 3 antibody, all related information, articles, resources about Caspase 3 antibody are being stored on our online database. Let us know if you have questions regarding this product.

Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed.

GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.

Thank you for visiting GenWay Biotech's Datasheet. GenWay is the world's leader in chicken, IgY antibody production, producing over 900 quality polyclonal IgY antibodies to date. From our unique recombinant protein production, utilizing a variety of protein expression systems, GenWay's polyclonal IgY antibodies incorporate more epitopes, increasing the success rate for ELISA, western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), and many more immunoassays.



GenWay Biotech, Inc. 6777 Nancy Ridge Drive, San Diego, CA 92121 Tel: (858)458-0866 Fax: (858)458-0833 Email: sales@genwaybio.com www.genwaybio.com ©1998 - 2010 GenWay Biotech, Inc. - All Rights Reserved