Notch1 antibody, Notch1 [A6] Antibody, Mouse Monoclonal Antibody

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Notch1 [A6] Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-272-192515

Buy Notch1 antibody -

Volume:

Related Product Names:

- Notch1 antibody; Notch1; TAN1, Translocation Associated Notch Homolog,; Notch1 [A6]
- Mouse monoclonal [A6] to Notch1; TAN1 Translocation Associated Notch Homolog; TAN1 Translocation Associated Notch Homolog

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: Notch1	Gene Name Synonym: TAN1, Translocation Associated Notch Homolog,
Gi #: N/A	NCBI Acc #: NP_060087.3	Swiss Prot Acc #: P46531
Length (aa): N/A	Mol. Weight (Da): 272505	Chrom Location: N/A

Clone: A6

Isotype: IgG2b

Immunogen: Recombinant protein encoding the ligand binding region of human Notch 1

Antigen Species: Human

Specificity: This antibody has not been tested for its cross reactivity with Notch 2.

Positive Control: Pancreas

Target: Notch1

Concentration: 0.2 mg/ml

Storage Buffer: Phosphate-buffered saline, pH7.4, containing 0.2% BSA and 0.1% sodium azide

Application Note: For FACS: Use at an assay dependent dilution. For IHC-P: Use at 1/50 for 30 minute at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at room temperature for 20 min. Not tested in other applications. Optimal dilutions / concentrations should be determined by the end user.

Source/Host: Mouse

Purity/Purification: Protein A affinity purified

Clonality: Monoclonal

Crossreactivity: Human and Mouse - Not yet tested in other species

Format: Liquid

Storage: Keep as concentrated solution, aliquot and store at 4C. Do not freeze.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for NOTCH1 ANTIBODY:

FACS, IHC-P: Tested

TESTING: (secondary reagents and protocols )

Not Available

NOTCH1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for Notch1 antibody: Notch1; TAN1, Translocation Associated Notch Homolog,; Mouse monoclonal [A6] to Notch1; TAN1 Translocation Associated Notch Homolog; TAN1 Translocation Associated Notch Homolog

The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine rich EGF like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch 1. notch 2, notch 3, and notch 4(Int 3), which have been implicated in the differentiation of the nervous system and other structures. The EGF like proteins Delta and Serrate have been identified as ligands of notch 1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch preproteins into an extracellular subunit (NEC) containing multiple EGF like repeats and a transmembrane subunit including intracellular region (Ntm). Alteration of notch 1 signaling or expression may contribute to tumorigenesis.

Function: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity).

Subunit: Heterodimer of a C-terminal fragment N(TM) and an N-terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1.

Subcellular Location: Cell membrane; Single-pass type I membrane protein (By similarity).

Subcellular Location: Notch 1 intracellular domain: Nucleus (By similarity). Note=Following proteolytical processing NICD is translocated to the nucleus (By similarity).

Tissue Specificity: In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.

Ptm: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).

Ptm: Phosphorylated (By similarity).

Disease: NOTCH1 truncation is associated with T-cell acute lymphoblastic leukemia.

Disease: Defects in NOTCH1 are a cause of aortic valve disease [MIM:109730]. The disorder consists of an early developmental defect in the aortic valve and a later de-repression of calcium deposition that causes progressive aortic valve disease. Calcification of the aortic valve is the third leading cause of heart disease in adults. The incidence increases with age, and it is often associated with a bicuspid aortic valve present in 1-2% of the population.

Similarity: Belongs to the NOTCH family.

Similarity: Contains 5 ANK repeats.

Similarity: Contains 36 EGF-like domains.

Similarity: Contains 3 LNR (Lin/Notch) repeats.

Notch1 [A6] reacts with human and mouse - not yet tested in other species .

OMIM: 109730; phenotype. [NCBI / EBI]
190198; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Dorso-ventral axis formation 04320
KEGG pathway: Notch signaling pathway 04330
Reactome Event:Signaling by Notch 157118

Products similar to Notch1 antibody:

IgG

NOTCH 1, IgG

Notch1 [SPM206] (ready-to-use), IgG

Anti-NOTCH 1 (Cleaved N terminal), IgG

Notch1, IgG

Anti-NOTCH 1 (intra), IgG

PRODUCT INTERACTIONS:

Notch1 [A6] interacts with recombining binding protein suppressor of hairless (Drosophila) (RBPSUH), IgG (GenWay Catalog #: 20-272-192515).

Notch1 [A6] interacts with RBPJ (recombination signal binding protein for immunoglobulin kappa J region), IgG (GenWay Catalog #: 20-272-192515).

Notch1 [A6] interacts with SKIIP, IgG (GenWay Catalog #: 20-272-192515).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: Notch1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of Notch1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about Notch1 antibody, all related information, articles, resources about Notch1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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