beta Catenin antibody, beta Catenin [15B8] Antibody, Mouse Monoclonal Antibody

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beta Catenin [15B8] Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-272-192649

Buy beta Catenin antibody -

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Related Product Names:

- beta Catenin antibody; beta Catenin; 1499; CTNNB1; 114550; P35222; CTNNB; 395964; CTNNB1; CHBCAT;; beta Catenin [15B8]
- Mouse monoclonal [15B8] to beta Catenin; 1499; CTNNB1; 114550; P35222; CTNNB; 395964; CTNNB1; CHBCAT;; Catenin beta-1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: beta Catenin	Gene Name Synonym: 1499; CTNNB1; 114550; P35222; CTNNB; 395964; CTNNB1; CHBCAT;
Gi #: N/A	NCBI Acc #: NP_001091679.1	Swiss Prot Acc #: P35222
Length (aa): N/A	Mol. Weight (Da): 85497	Chrom Location: N/A

Clone: 15B8

Isotype: IgG1

Immunogen: Recombinant full length protein (Chicken).

Antigen Species: Chicken

Specificity: Recognises beta-catenin in immunoblotting (94kD). Does not recognise plakoglobin.

Positive Control: Cultured MDBK cells or cell extract

Target: beta Catenin

Localization: Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation).

Storage Buffer: Ascitic fluid with 0.1% sodium azide

Application Note: ICC: Use at an assay dependant dilution. IF: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular Localization: Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation).

Source/Host: Mouse

Purity/Purification: Ascites

Clonality: Monoclonal

Crossreactivity: Cross-reacts with Cow, Dog and Human. Not yet tested in other species.

Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for BETA CATENIN ANTIBODY:

ICC, IF, WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

BETA CATENIN ANTIBODY TARGET DESCRIPTION:

Synonym Names for beta Catenin antibody: beta Catenin; 1499; CTNNB1; 114550; P35222; CTNNB; 395964; CTNNB1; CHBCAT;; Mouse monoclonal [15B8] to beta Catenin; 1499; CTNNB1; 114550; P35222; CTNNB; 395964; CTNNB1; CHBCAT;; Catenin beta-1

The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kD) found in many tissues (1,2,3) bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. (6,7) They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. (4,5) Absence of alpha-catenin is found in certain tumor cell lines (8) and reduced levels in certain human carcinomas (9). Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. (10) This interaction involves a 15-aa repeat in the APC. (11) Beta-catenin cell levels seem to be controlled by APC. (12) The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR. (13) .

Function: Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

Subunit: Two separate pools are found in the cytoplasm: one is PSEN1/cadherin/catenin complex which anchors to the actin cytoskeleton. The other pool is part of a large complex containing AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. Wnt-dependent activation of DVL antagonizes the action of GSK3B. When GSK3B activity is inhibited the complex dissociates, CTNNB1 is dephosphorylated and is no longer targeted for destruction. The stabilized protein translocates to the nucleus where it binds TCF/LEF-1 family members, TBP, BCL9 and possibly also RUVBL1 and DUPLIN. Binds CTNNBIP and EP300. CTNNB1 forms a ternary complex with LEF1 and EP300 that is disrupted by CTNNBIP1 binding (By similarity). Interacts with TAX1BP3 (via the PDZ domain); this interaction inhibits the transcriptional activity of CTNNB1 (By similarity). Interacts with AJAP1, BAIAP1, CARM1, CTNNA3, CXADR and PCDH11Y. Binds SLC9A3R1. Interacts with GLIS2 and MUC1. Interacts with SLC30A9. Interacts with XIRP1 (By similarity). Interacts with PTPRU (via the cytoplasmic juxtamembrane domain).

Subcellular Location: Cytoplasm. Nucleus. Cytoplasm, cytoskeleton. Note=Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation.

Tissue Specificity: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheats. Expressed in colon.

Ptm: Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.

Ptm: EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.

Ptm: Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X (Probable). Its ubiquitination leads to its subsequent proteasomal degradation.

Disease: Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.

Disease: Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.

Disease: Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.

Disease: A chromosomal rearrangement involving CTNNB1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.

Similarity: Belongs to the beta-catenin family.

Similarity: Contains 12 ARM repeats.

beta Catenin [15B8] reacts with cross-reacts with cow, dog and human. not yet tested in other species. .

OMIM: 116806; gene. [NCBI / EBI]
132600; phenotype. [NCBI / EBI]
155255; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Adherens junction 04520
KEGG pathway: Basal cell carcinoma 05217
KEGG pathway: Colorectal cancer 05210
KEGG pathway: Endometrial cancer 05213
KEGG pathway: Focal adhesion 04510
KEGG pathway: Leukocyte transendothelial migration 04670
KEGG pathway: Melanogenesis 04916
KEGG pathway: Pathogenic Escherichia coli infection - EHEC 05130
KEGG pathway: Pathogenic Escherichia coli infection - EPEC 05131
KEGG pathway: Prostate cancer 05215
KEGG pathway: Thyroid cancer 05216
KEGG pathway: Tight junction 04530
KEGG pathway: Wnt signaling pathway 04310
Reactome Event:Apoptosis 109581
Reactome Event:Signaling by Wnt 195721

Products similar to beta Catenin antibody:

IgG

beta Catenin [6F9], IgG

beta Catenin phospho S33 [BC-76], IgG

beta Catenin, IgG

Catenin beta,(exon 3), IgG

Catenin beta, pTyr 86, IgG

Peptide

beta-Catenin (Phospho-Thr41/Ser45) Antibody Blocking Peptide, Peptide

beta-Catenin (Phospho-Ser37) Antibody Blocking Peptide, Peptide

beta-Catenin (Phospho-Ser33) Antibody Blocking Peptide, Peptide

PRODUCT INTERACTIONS:

beta Catenin [15B8] interacts with ACTN4 (actinin. alpha 4), IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with Anti-Dihydrotestosterone, IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with B-cell CLL/lymphoma 9 (BCL9), IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with E-cadherin, Reagents (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with Cadherin-1 (E-cadherin), IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with E Cadherin [67A4], IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with pan Cadherin [CH-19], IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with Human E-cadherin EIA Kit, ELISA_Kits (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with E-Cadherin, IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with Cadherin-E Human, Protein (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with E Cadherin [DECMA-1], IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with pan Cadherin [CH-19] (FITC), IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with pan Cadherin (ready-to-use), IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with pan Cadherin, IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with E Cadherin (ready-to-use), IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with E Cadherin, IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with CD324, IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with FGFR1, IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with FGFR1 [M19B2], IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with FGFR1 [M5G10], IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with FGFR1 [M17D10], IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with FGFR1 alpha [M2F12], IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with FGFR1 alpha [M1C4], IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with FGFR1 beta [M17A3], IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with FGF Receptor 1 alpha, IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with FGF Receptor 1 beta, IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with FGFR1 Human, Protein (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with NDRG1 (N-myc downstream regulated gene 1), IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with PRESENILIN-1, IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with Presenilin 1 (ready-to-use), IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with Rabbit anti Presenilin-1, IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with Goat A' Presenilin-2 (N-terminal), IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with Goat A' Presenilin-1 (N-terminal), IgG (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with Presenilin-1 N-terminal Peptide, Peptide (GenWay Catalog #: 20-272-192649).

beta Catenin [15B8] interacts with Presenilin 1, IgG (GenWay Catalog #: 20-272-192649).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: beta Catenin antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of beta Catenin antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about beta Catenin antibody, all related information, articles, resources about beta Catenin antibody are being stored on our online database. Let us know if you have questions regarding this product.

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