TLR4 antibody, TLR4 [MTS510] (Biotin) Antibody, Rat Monoclonal Antibody

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TLR4 [MTS510] (Biotin) Antibody, Rat Monoclonal Antibody

Catalog Number: 20-272-192974

Buy TLR4 antibody -

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Related Product Names:

- TLR4 antibody; TLR4; Homolog of Drosophila toll, hToll, Toll (Drosophila) homolog, TOLL, Toll Endotoxin Hyporesponsiveness, Toll like receptor 4 prec; TLR4 [MTS510] (Biotin)
- Rat monoclonal [MTS510] to TLR4 ( Biotin ); 21898; Tlr4; Lps; Ly87; Ran/M1; Rasl2-8;; 21898; Tlr4; Lps; Ly87; Ran/M1; Rasl2-8;

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: TLR4	Gene Name Synonym: Homolog of Drosophila toll, hToll, Toll (Drosophila) homolog, TOLL, Toll Endotoxin Hyporesponsiveness, Toll like receptor 4 prec
Gi #: N/A	NCBI Acc #: NP_612564.1	Swiss Prot Acc #: O00206
Length (aa): N/A	Mol. Weight (Da): 95680	Chrom Location: N/A

Clone: MTS510

Isotype: IgG2a

Immunogen: Mouse Ba/F3 cells expressing mouse TLR4/MD-2

Antigen Species: Mouse

Specificity: Only detects murine TLR-4 when complexed to MD-2.

Target: TLR4

Localization: Type I membrane protein.

Conjugation: Biotin

Concentration: 0.5 mg/ml

Storage Buffer: PBS with 0.1% BSA 0.02% sodium azide pH7.2

Application Note: The antibody recognizes the murine TLR4/MD-2 complex and is not useful for detection of TLR4 alone. The antibody can be used for flow cytometry and immunoprecipitation. Furthermore the antibody can be used as antagonist for TLR4, it blocks LPS-induced cytokine production.FLOW: 2-5 ug/ 1x 10^6 cellsIP: 2-5 ug/ ml: Please see Akashi et al (2000) for detailsFunctional Studies: Please see Akashi et al (2000) for details

Cellular Localization: Type I membrane protein.

Source/Host: Rat

Purity/Purification: IgG fraction

Clonality: Monoclonal

Crossreactivity: Cross-reacts with Mouse. Not yet tested in other species.

Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for TLR4 ANTIBODY:

FACS, FuncS, IP: Tested

TESTING: (secondary reagents and protocols )

Not Available

TLR4 ANTIBODY TARGET DESCRIPTION:

Synonym Names for TLR4 antibody: TLR4; Homolog of Drosophila toll, hToll, Toll (Drosophila) homolog, TOLL, Toll Endotoxin Hyporesponsiveness, Toll like receptor 4 prec; Rat monoclonal [MTS510] to TLR4 ( Biotin ); 21898; Tlr4; Lps; Ly87; Ran/M1; Rasl2-8;; 21898; Tlr4; Lps; Ly87; Ran/M1; Rasl2-8;

Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram-negative bacteria. The MTS510 monoclonal antibody reacts preferentially, especially in flow cytometry, with murine TLR4 that is associated with MD-2. MTS510 is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to co-precipitate MD-2 (30 kDa) with TLR4 (100 kDa).

Function: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.

Subunit: Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4.

Subcellular Location: Membrane; Single-pass type I membrane protein.

Tissue Specificity: Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

Domain: The TIR domain mediates interaction with NOX4.

Ptm: N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.

Polymorphism: Allele TLR4*B (Gly-299, Ile-399) is associated with a blunted response to inhaled LPS.

Disease: Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.

Similarity: Belongs to the Toll-like receptor family.

Similarity: Contains 21 LRR (leucine-rich) repeats.

Similarity: Contains 1 TIR domain.

TLR4 [MTS510] (Biotin) reacts with cross-reacts with mouse. not yet tested in other species..

OMIM: 603030; gene+phenotype. [NCBI / EBI]
611488; phenotype. [NCBI / EBI]

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TLR4 [HTA125] (Biotin), IgG

PRODUCT INTERACTIONS:

TLR4 [MTS510] (Biotin) interacts with Toll-interleukin 1 receptor domain-containing adapter protein, IgG (GenWay Catalog #: 20-272-192974).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

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Precautions: TLR4 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of TLR4 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about TLR4 antibody, all related information, articles, resources about TLR4 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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