ATM antibody, ATM [3E8] Antibody, Mouse Monoclonal Antibody

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ATM [3E8] Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-272-193220

Buy ATM antibody -

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Related Product Names:

- ATM antibody; ATM; ataxia telangiectasia, AT, ATR, PI3, phosphatidylinositol; ATM [3E8]
- Mouse monoclonal [3E8] to ATM; 472; ATM; 114480; Q13315; AT1; ATA; ATC; ATD; ATDC; ATE; DKFZp781A0353; MGC74674; TEL1;; 472; ATM; 114480; Q13315; AT1; ATA; ATC; ATD; ATDC; ATE; DKFZp781A0353; MGC74674; TEL1;

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: ATM	Gene Name Synonym: ataxia telangiectasia, AT, ATR, PI3, phosphatidylinositol
Gi #: N/A	NCBI Acc #: NP_000042.3	Swiss Prot Acc #: Q13315
Length (aa): N/A	Mol. Weight (Da): 350644	Chrom Location: N/A

Clone: 3E8

Isotype: IgG1

Light chain: kappa

Immunogen: Recombinant protein expressed in E. coli corresponding to amino acids 980-1512.

Antigen Species: Human

Positive Control: Raji, HeLa, or Akata

Target: ATM

Localization: Nuclear

Concentration: 1 mg/ml

Purification Note: From Ascites

Storage Buffer: Phosphate-buffered saline, pH7.4, containing no preservatives

Application Note: For IP: Use at a concentration of 2-10 µg/ml. For WB: Use at a concentration of 1-2 µg/ml. Predicted moolecular weight: 350kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.

Source/Host: Mouse

Purity/Purification: Protein G purified

Clonality: Monoclonal

Crossreactivity: Human, Rat, Mouse and Monkey - Not yet tested in other species

Format: Liquid

Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for ATM ANTIBODY:

IP, WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

ATM ANTIBODY TARGET DESCRIPTION:

Synonym Names for ATM antibody: ATM; ataxia telangiectasia, AT, ATR, PI3, phosphatidylinositol; Mouse monoclonal [3E8] to ATM; 472; ATM; 114480; Q13315; AT1; ATA; ATC; ATD; ATDC; ATE; DKFZp781A0353; MGC74674; TEL1;; 472; ATM; 114480; Q13315; AT1; ATA; ATC; ATD; ATDC; ATE; DKFZp781A0353; MGC74674; TEL1;

ATM-3E8 recognizes full-length ATM, a 370kDa nuclear phosphoprotein, which is involved in the autosomal recessive disease ataxia telangiectasia (AT). ATM belongs to a novel family of proteins associated with cell cycle regulation and response to DNA repair. The C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Its usefulness to monitor altered ATM expression in cancer is under active investigation.

Function: Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function.

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme Regulation: Inhibited by wortmannin.

Subunit: Exists in monomeric and tetrameric state. Binds DNA ends, p53/TP53, ABL1, BRCA1, NBN/nibrin and TERF1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. Interacts with EEF1E1. This interaction, which takes place independently of TP53, is induced by DNA damage that may occur during genotoxic stress or cell growth. Interacts with DCLRE1C. Interacts with MYST1. Interacts with HTATIP. Interacts with OBFC2B. Interacts with ATMIN.

Subcellular Location: Nucleus. Cytoplasmic vesicle. Note=Primarily nuclear. Found also in endocytic vesicles in association with beta-adaptin.

Tissue Specificity: Found in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, spleen, thymus, testis, ovary, small intestine, colon and leukocytes.

Induction: By ionizing radiation.

Domain: The FATC domain is required for interaction with HTATIP.

Ptm: Phosphorylated by ARK5. Autophosphorylated on Ser-1981 upon DNA damage.

Ptm: Acetylated by HTATIP upon DNA damage; which is required for autophosphorylation and subsequent activation.

Disease: Defects in ATM are the cause of ataxia telangiectasia (AT) [MIM:208900]; also known as Louis-Bar syndrome, which includes four complementation groups: A, C, D and E. This rare recessive disorder is characterized by progressive cerebellar ataxia, dilation of the blood vessels in the conjunctiva and eyeballs, immunodeficiency, growth retardation and sexual immaturity. AT patients have a strong predisposition to cancer; about 30% of patients develop tumors, particularly lymphomas and leukemias. Cells from affected individuals are highly sensitive to damage by ionizing radiation and resistant to inhibition of DNA synthesis following irradiation.

Disease: Defects in ATM contribute to T-cell acute lymphoblastic leukemia (TALL) and T-prolymphocytic leukemia (TPLL). TPLL is characterized by a high white blood cell count, with a predominance of prolymphocytes, marked splenomegaly, lymphadenopathy, skin lesions and serous effusion. The clinical course is highly aggressive, with poor response to chemotherapy and short survival time. TPLL occurs both in adults as a sporadic disease and in younger AT patients.

Disease: Defects in ATM contribute to B-cell non-Hodgkin lymphomas (BNHL), including mantle cell lymphoma (MCL).

Disease: Defects in ATM contribute to B-cell chronic lymphocytic leukemia (BCLL). BCLL is the commonest form of leukemia in the elderly. It is characterized by the accumulation of mature CD5+ B lymphocytes, lymphadenopathy, immunodeficiency and bone marrow failure.

Similarity: Belongs to the PI3/PI4-kinase family. ATM subfamily.

Similarity: Contains 1 FAT domain.

Similarity: Contains 1 FATC domain.

Similarity: Contains 1 PI3K/PI4K domain.

ATM [3E8] reacts with human, rat, mouse and monkey - not yet tested in other species.

OMIM: 208900; phenotype. [NCBI / EBI]
607585; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Apoptosis 04210
KEGG pathway: Cell cycle 04110
Reactome Event:Cell Cycle Checkpoints 69620
Reactome Event:DNA Repair 73894

Products similar to ATM antibody:

IgG

ATM, IgG

ATM [2C1] (supernatant), IgG

ATR [2B5], IgG

ATM [3E8], IgG

ATM phospho S1981, IgG

PRODUCT INTERACTIONS:

ATM [3E8] interacts with AATF (apoptosis antagonizing transcription factor), IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with apoptosis antagonizing transcription factor, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with AATF (apoptosis antagonizing transcription factor), IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with c-Abl phospho Y245, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with c-Abl (Phospho-Tyr412), IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with c-Abl (Ab-412), IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with c-Abl (Phospho-Tyr412) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with Herpes Type 6 p150, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with c-Abl (ready-to-use), IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with c-Abl, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with c-Abl [19-110], IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with c-Abl [ABL-148], IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with c-Abl phospho Y412, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with c-Abl, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with MDC1, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with Anti-MDC1, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with MDC, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with SMC1, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with SMC1 (Phospho-Ser957), IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with SMC1 (Ab-957), IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with SMC1 (Phospho-Ser957) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with SMC1, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with SMC1 phospho S966, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with SMC1 phospho S957, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with TRF1, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with TRF1 [3H11], IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with TRF1 [4E4], IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with TRF1 [TRF-78], IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with p53, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with TRP53, IgG (GenWay Catalog #: 20-272-193220).

ATM [3E8] interacts with Anti p53, IgG (GenWay Catalog #: 20-272-193220).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

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Precautions: ATM antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of ATM antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about ATM antibody, all related information, articles, resources about ATM antibody are being stored on our online database. Let us know if you have questions regarding this product.

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