NBS1 antibody, NBS1 [1D7] Antibody, Mouse Monoclonal Antibody

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NBS1 [1D7] Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-272-193314

Buy NBS1 antibody -

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Related Product Names:

- NBS1 antibody; NBS1; NBS, p95, nibrin, nibrin breakage syndrome, MRN, hNBS1, hNBS; NBS1 [1D7]
- Mouse monoclonal [1D7] NBS1; NBS p95 nibrin nibrin breakage syndrome MRN hNBS1 hNBS; NBS p95 nibrin nibrin breakage syndrome MRN hNBS1 hNBS

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: NBS1	Gene Name Synonym: NBS, p95, nibrin, nibrin breakage syndrome, MRN, hNBS1, hNBS
Gi #: N/A	NCBI Acc #: NP_001019859.1	Swiss Prot Acc #: O60934
Length (aa): N/A	Mol. Weight (Da): 84959	Chrom Location: N/A

Clone: 1D7

Isotype: IgG1

Light chain: kappa

Immunogen: The complete coding region of the human p95/NBS1 expressed in E. coli.

Antigen Species: Human

Positive Control: MCF7, HeLa, Raji

Target: NBS1

Concentration: 1 mg/ml

Purification Note: From ascitic fluid

Storage Buffer: Phosphate-buffered saline, pH 7.4, containing no preservatives

Application Note: For IP: Use at an assay dependent dilution. For WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 91 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.

Source/Host: Mouse

Purity/Purification: Protein G Affinity Purified

Clonality: Monoclonal

Crossreactivity: Human. Others not determined.

Format: Liquid

Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for NBS1 ANTIBODY:

IP, WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

NBS1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for NBS1 antibody: NBS1; NBS, p95, nibrin, nibrin breakage syndrome, MRN, hNBS1, hNBS; Mouse monoclonal [1D7] NBS1; NBS p95 nibrin nibrin breakage syndrome MRN hNBS1 hNBS; NBS p95 nibrin nibrin breakage syndrome MRN hNBS1 hNBS

Nijmegen breakage syndrome (NBS, nibrin) is an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The 1C3 antibody recognizes the 95 kDa NBS protein which contains a fork FHA head-associated domain that is adjacent to BRCT, a breast cancer C terminal domain, that has been implicated in protein-protein interactions. NBS is member of the Mre11/Rad50, p400 double-strand break repair complex involved in double-strand break repair and cell-cycle checkpoint functions.

Function: Component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability.

Subunit: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50 and MRE11A (By similarity). Interacts with histone H2AFX this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with HJURP, KPNA2 and TERF2.

Subcellular Location: Nucleus (By similarity). Telomere (By similarity). Note=Localizes to discrete nuclear foci after treatment with genotoxic agents (By similarity).

Tissue Specificity: Ubiquitous. Expressed at high levels in testis.

Domain: The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.

Domain: The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.

Domain: The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.

Ptm: Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.

Disease: Defects in NBN are the cause of Nijmegen breakage syndrome (NBS) [MIM:251260]. NBS is an autosomal recessive syndrome characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer, particularly to lymphoid malignancies.

Disease: Defects in NBN are a cause of genetic susceptibility to breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer.

Disease: Defects in NBN may be associated with aplastic anemia [MIM:609135]. Aplastic anemia is a disease of bone-marrow failure characterized by peripheral pancytopenia and marrow hypoplasia. Most of the cases of aplastic anemia are idiopathic, some are familial and some are due to a viral infection or to exposure to chemicals and radiation.

Disease: Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).

Miscellaneous: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.

Similarity: Contains 1 BRCT domain.

Similarity: Contains 1 FHA domain.

NBS1 [1D7] reacts with human. others not determined..

OMIM: 114480; phenotype. [NCBI / EBI]
251260; phenotype. [NCBI / EBI]
602667; gene. [NCBI / EBI]
609135; phenotype. [NCBI / EBI]

Products similar to NBS1 antibody:

IgG

NBS1 phospho S343, IgG

p95/NBS1 (Ab-343), IgG

NBS1 [1C3], IgG

Rabbit anti NALP2, IgG

p95/NBS1 (Phospho-Ser343), IgG

Peptide

p95/NBS1 (Phospho-Ser343) Antibody Blocking Peptide, Peptide

PRODUCT INTERACTIONS:

NBS1 [1D7] interacts with G1/S-specific cyclin-E1, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with CYCLIN E, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with Cyclin E (ready-to-use), IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with Cyclin E [HE12], IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with Rabbit anti Cyclin E, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with Cyclin E, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with HISTONE H2AX, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with PHOSPHORYLATED HISTONE H2AX (GAMMA-H2AX), IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with Histone H2A.X (Phospho-Ser139), IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with Histone H2A.X (Ab-139), IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with Histone H2A.X (Phospho-Ser139) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with H2A.X, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with H2A.X phospho S139, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with H2A.X, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with MDC1, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with Anti-MDC1, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with MDC, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with MRE11, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with Mre11, IgG (GenWay Catalog #: 20-272-193314).

NBS1 [1D7] interacts with Mre11 [12D7], IgG (GenWay Catalog #: 20-272-193314).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

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Precautions: NBS1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of NBS1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about NBS1 antibody, all related information, articles, resources about NBS1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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