RbAp48 antibody, RbAp48 [11G10] Antibody, Mouse Monoclonal Antibody

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RbAp48 [11G10] Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-272-193322

Buy RbAp48 antibody -

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Related Product Names:

- RbAp48 antibody; RbAp48; Rb associated protein 48, Rb-associated protein, RbAp 48, Chromatin assembly factor 1 subunit C, CAF-1 subunit C, Chromatin asse; RbAp48 [11G10]
- Mouse monoclonal antibody to human RbAp48; 5928; RBBP4; 602923; Q09028; RBAP48;; 5928; RBBP4; 602923; Q09028; RBAP48;

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: RbAp48	Gene Name Synonym: Rb associated protein 48, Rb-associated protein, RbAp 48, Chromatin assembly factor 1 subunit C, CAF-1 subunit C, Chromatin asse
Gi #: N/A	NCBI Acc #: NP_005601.1	Swiss Prot Acc #: Q09028
Length (aa): N/A	Mol. Weight (Da): 47656	Chrom Location: N/A

Clone: 11G10

Isotype: IgG2b

Light chain: kappa

Immunogen: The complete coding region (amino acids 1-425) of RbAp48 expressed in E. coli.

Antigen Species: Human

Positive Control: MOLT4, HeLa, LB22

Target: RbAp48

Localization: Nuclear

Concentration: 1 mg/ml

Purification Note: From hybridoma tissue culture supernatant

Storage Buffer: Phosphate-buffered saline, pH 7.4, containing no preservatives

Application Note: For IF: Permeabilization step recommended. Use at 1:500-1:1000 in PBST containing 2%BSA and 10% normal serum at 4ºC overnight. For WB: Use at a dilution of 1:500 -1:2,000 in 5% Milk/PBST at 4ºC overnight. Use under reducing conditions. For IP: se at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.

Source/Host: Mouse

Purity/Purification: Protein G Affinity Purified

Clonality: Monoclonal

Crossreactivity: Human and Mouse - Not yet tested in other species

Format: Liquid

Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for RBAP48 ANTIBODY:

IF, IP, WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

RBAP48 ANTIBODY TARGET DESCRIPTION:

Synonym Names for RbAp48 antibody: RbAp48; Rb associated protein 48, Rb-associated protein, RbAp 48, Chromatin assembly factor 1 subunit C, CAF-1 subunit C, Chromatin asse; Mouse monoclonal antibody to human RbAp48; 5928; RBBP4; 602923; Q09028; RBAP48;; 5928; RBBP4; 602923; Q09028; RBAP48;

RbAp48-11G10 recognizes full length RbAp48, a 48 kDa nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.

Function: Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.

Subunit: Interacts with SUV39H1 and HDAC7 (By similarity). Binds directly to helix 1 of the histone fold of histone H4, a region that is not accessible when H4 is in chromatin. Subunit of the chromatin assembly factor 1 (CAF-1) complex, which is composed of RBBP4, CHAF1B and CHAF1A. Subunit of the core histone deacetylase (HDAC) complex, which is composed of HDAC1, HDAC2, RBBP4 and RBBP7. The core HDAC complex associates with SIN3A, ARID4B/SAP180, SAP18, SAP30, SAP130, SDS3/SAP45 and possibly ARID4A/RBP1 and ING1 to form the SIN3 HDAC complex. The core HDAC complex may also associate with MTA2, MBD3, CHD3 and CHD4 to form the nucleosome remodeling and histone deacetylase complex (the NuRD complex). The NuRD complex may also interact with MBD3L1 and MBD3L2. Interacts with MTA1. Subunit of the PRC2/EED-EZH2 complex, which is composed of at least EED, EZH2, RBBP4, RBBP7 and SUZ12. The PRC2/EED-EZH2 complex may also associate with HDAC1. Part of the nucleosome remodeling factor (NURF) complex which consists of SMARCA1; BPTF; RBBP4 and RBBP7. Interacts with the viral protein-binding domain of the retinoblastoma protein (RB1). Interacts with SPEN/MINT. Interacts with BRCA1. Interacts with CREBBP, and this interaction may be enhanced by the binding of phosphorylated CREB1 to CREBBP. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2.

Subcellular Location: Nucleus.

Similarity: Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.

Similarity: Contains 6 WD repeats.

RbAp48 [11G10] reacts with human and mouse - not yet tested in other species .

OMIM: 602923; gene. [NCBI / EBI]

Products similar to RbAp48 antibody:

IgG

RbAp48 [15G12], IgG

RbAp48 [13D10], IgG

RbAp48 + RbAp46 [15G12], IgG

RbAp48 [11G10], IgG

RbAp48 [13D10], IgG

PRODUCT INTERACTIONS:

RbAp48 [11G10] interacts with HDAC1 (histone deacetylase 1), IgG (GenWay Catalog #: 20-272-193322).

RbAp48 [11G10] interacts with HDAC1 [HDAC1-21], IgG (GenWay Catalog #: 20-272-193322).

RbAp48 [11G10] interacts with Anti-HDAC-1, IgG (GenWay Catalog #: 20-272-193322).

RbAp48 [11G10] interacts with HDAC1, IgG (GenWay Catalog #: 20-272-193322).

RbAp48 [11G10] interacts with HDAC1 (ready-to-use), IgG (GenWay Catalog #: 20-272-193322).

RbAp48 [11G10] interacts with HDAC1, IgG (GenWay Catalog #: 20-272-193322).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: RbAp48 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of RbAp48 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about RbAp48 antibody, all related information, articles, resources about RbAp48 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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