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| CD45 [YW 62.3] (FITC) Antibody, Rat Monoclonal Antibody |
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| Catalog Number: 20-272-193622 |
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| Related Product Names:
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CD45 antibody; CD45; 19264; Ptprc; B220; CD45R; Cd45; Ly-5; Lyt-4; T200; loc; 5788; PTPRC; 126200; P08575; B220; CD45; GP180; LCA; LY5; T200;; CD45 [YW 62.3] (FITC)
- Rat Antibody to Mouse CD45 ( Ly - 5 ):FITC; 19264; Ptprc; B220; CD45R; Cd45; Ly-5; Lyt-4; T200; loc; 5788; PTPRC; 126200; P08575; B220; CD45; GP180; LCA; LY5; T200;; Leukocyte common antigen |
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- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
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| Gene Name: CD45 |
Gene Name Synonym: 19264; Ptprc; B220; CD45R; Cd45; Ly-5; Lyt-4; T200; loc; 5788; PTPRC; 126200; P08575; B220; CD45; GP180; LCA; LY5; T200; |
Gi #:
N/A |
NCBI Acc #:
NP_002829.2 |
Swiss Prot Acc #:
P08575 |
Length (aa): N/A |
Mol. Weight (Da): 147254 |
Chrom Location: N/A |
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 Clone: YW 62.3
Isotype: IgG2b
Immunogen: Mouse spleen cells.
Antigen Species: Mouse
Specificity: murine CD45
Target: CD45
Conjugation: FITC
Concentration: 0.1 mg/ml
Storage Buffer: Phosphate buffered saline
Application Note: Flow cytometry; Flow Cytometry:Use 10ul of the suggested working dilution to label 10^6 cells in 100ul.
Myeloma: Spleen cells from immunised DA rat were fused with cells of the Y3/Ag1.2.3 myeloma cell line.
Source/Host: Rat
Purity/Purification: Purified IgG
Clonality: Monoclonal
Crossreactivity: some reactivity with human tissue has been observed
Format: Liquid
Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use. This product contains sodium azide.
Shipping: Products may be shipped on ice pack or dry ice. |
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| APPLICATIONS for CD45 ANTIBODY: |
| FACS: Tested |
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| CD45 ANTIBODY TARGET DESCRIPTION: |
Synonym Names for CD45 antibody: CD45; 19264; Ptprc; B220; CD45R; Cd45; Ly-5; Lyt-4; T200; loc; 5788; PTPRC; 126200; P08575; B220; CD45; GP180; LCA; LY5; T200;; Rat Antibody to Mouse CD45 ( Ly - 5 ):FITC; 19264; Ptprc; B220; CD45R; Cd45; Ly-5; Lyt-4; T200; loc; 5788; PTPRC; 126200; P08575; B220; CD45; GP180; LCA; LY5; T200;; Leukocyte common antigen
Mouse CD45 monomorphic antigen, expressed on most haemopoietic cells (1,2). Immunoprecipitates multiple isoforms with molecular weights 180-220kD.
Function: Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.
Catalytic Activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Subunit: Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.
Subcellular Location: Membrane; Single-pass type I membrane protein.
Domain: The first PTPase domain interacts with SKAP1.
Ptm: Heavily N- and O-glycosylated.
Disease: Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+)SCID) [MIM:608971]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Disease: Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain.
Similarity: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
Similarity: Contains 2 fibronectin type-III domains.
Similarity: Contains 2 tyrosine-protein phosphatase domains.
CD45 [YW 62.3] (FITC) reacts with some reactivity with human tissue has been observed.
OMIM: 126200; phenotype. [NCBI / EBI]
151460; gene. [NCBI / EBI]
608971; phenotype. [NCBI / EBI]
Pathways:
KEGG pathway: Cell adhesion molecules (CAMs) 04514
KEGG pathway: T cell receptor signaling pathway 04660
Reactome Event:Signaling in Immune system 168256
Products similar to CD45 antibody:
IgG
 CD18 [MEM-48], IgG
Mouse Anti-Human CD45RA, IgG
Rat Anti-Mouse CD45RC, IgG
CD45RA, IgG
CD45RO, IgG
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SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: CD45 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of CD45 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about CD45 antibody, all related information, articles, resources about CD45 antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
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