F-actin antibody, F-actin [NH3] Antibody, Mouse Monoclonal Antibody

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F-actin [NH3] Antibody, Mouse Monoclonal Antibody

Catalog Number: 20-272-193885

Buy F-actin antibody -

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Related Product Names:

- F-actin antibody; F-actin; F-actin [NH3]
- Mouse monoclonal [NH3] to human actin sample; Actin, cytoplasmic 1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: F-actin	Gene Name Synonym: N/A
Gi #: N/A	NCBI Acc #: NP_001092.1	Swiss Prot Acc #: P60709
Length (aa): N/A	Mol. Weight (Da): 41737	Chrom Location: N/A

Clone: NH3

Isotype: IgM

Immunogen: Human monocytes and U937 cell line

Antigen Species: Human

Specificity: GTX76100 recognises human Filamentous actin (F-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types.

Target: F-actin

Concentration: 1.0 mg/ml

Purification Note: From hybridoma culture supernatant

Storage Buffer: Phosphate buffered saline pH 7.4, containing 0.09% sodium azide

Application Note: For Flow cytometry: Use at a dilution of 1:5 - 1:25. For IHC-Fr: Use at an assay dependent dilution. Not tested in other applications. Optimal concentrations/dilutions should be determined by the researcher.

Myeloma: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Source/Host: Mouse

Purity/Purification: Purified IgM

Clonality: Monoclonal

Crossreactivity: Rabbit - Not yet tested in other species

Format: Liquid

Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for F-ACTIN ANTIBODY:

FACS, IHC-Fr: Tested

TESTING: (secondary reagents and protocols )

Not Available

F-ACTIN ANTIBODY TARGET DESCRIPTION:

Synonym Names for F-actin antibody: F-actin; Mouse monoclonal [NH3] to human actin sample; Actin, cytoplasmic 1

Filamentous (F) actin is a principle cytoskeletal element in some non-muscle cells including polymorphonuclear leukocytes (PMNs).

Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Subunit: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with XPO6.

Subcellular Location: Cytoplasm, cytoskeleton.

Disease: Defects in ACTB are a cause of juvenile-onset dystonia [MIM:607371]. Dystonia is a movement disorder with a neurological basis, due to disordered tonicity of muscle. It is characterized by sustained involuntary muscle contractions that cause abnormal postures and repetitive movements. It may affect muscles throughout the body (generalized), in certain parts of the body (segmental), or may be confined to particular muscles or muscle groups (focal). Individuals with juvenile-onset dystonia due to ACTB mutations present a complicated phenotype that includes progressive generalized dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.

Miscellaneous: In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility.

Similarity: Belongs to the actin family.

F-actin [NH3] reacts with rabbit - not yet tested in other species.

OMIM: 102630; gene. [NCBI / EBI]
607371; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Adherens junction 04520
KEGG pathway: Cell Communication 01430
KEGG pathway: Focal adhesion 04510
KEGG pathway: Leukocyte transendothelial migration 04670
KEGG pathway: Pathogenic Escherichia coli infection - EHEC 05130
KEGG pathway: Pathogenic Escherichia coli infection - EPEC 05131
KEGG pathway: Regulation of actin cytoskeleton 04810
KEGG pathway: Tight junction 04530

PRODUCT INTERACTIONS:

F-actin [NH3] interacts with Cofilin 2 phospho S3 antibody, IgG (GenWay Catalog #: 20-272-193885).

F-actin [NH3] interacts with Cofilin 2, IgG (GenWay Catalog #: 20-272-193885).

F-actin [NH3] interacts with MDM2 [2A10], IgG (GenWay Catalog #: 20-272-193885).

F-actin [NH3] interacts with MDM2 [SMP14], IgG (GenWay Catalog #: 20-272-193885).

F-actin [NH3] interacts with Rabbit anti Mouse MDM2 pS185, IgG (GenWay Catalog #: 20-272-193885).

F-actin [NH3] interacts with MDM2 [HDM2-323], IgG (GenWay Catalog #: 20-272-193885).

F-actin [NH3] interacts with MDM2 [MD-219], IgG (GenWay Catalog #: 20-272-193885).

F-actin [NH3] interacts with MDM2 (ready-to-use), IgG (GenWay Catalog #: 20-272-193885).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: F-actin antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of F-actin antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about F-actin antibody, all related information, articles, resources about F-actin antibody are being stored on our online database. Let us know if you have questions regarding this product.

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