Jagged 1 antibody, Jagged 1 Antibody, Goat Polyclonal Antibody

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Jagged 1 Antibody, Goat Polyclonal Antibody

Catalog Number: 18-272-195143

Buy Jagged 1 antibody -

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Related Product Names:

- Jagged 1 antibody; Jagged 1; JAGL1; Jagged 1
- Goat polyclonal to Jagged 1; Protein jagged-1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: Jagged 1	Gene Name Synonym: JAGL1
Gi #: N/A	NCBI Acc #: NP_000205.1	Swiss Prot Acc #: P78504
Length (aa): N/A	Mol. Weight (Da): 133799	Chrom Location: N/A

Isotype: IgG

Immunogen: Recombinant rat jagged-1 extracellular domain expressed in mouse NSO cells.

Antigen Species: Mouse

Target: Jagged 1

Concentration: 0.1 mg/ml

Purification Note: The antibody is purified using rat jagged 1 affinity chromatography.

Storage Buffer: Preservative: None Constituents: PBS

Application Note: ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit for recombinant rat Jagged-1 is approximately 0.16 ng/well. FACS: Use at a concentration of 20 µg/ml. (10 µl/10^5 - 10^6 cells) this concentration is recommended to label human Jagged 1 transfected FBM stromal cells. Neut: Use at a concentration of 2 - 10 µg/ml. The antibody will block 50% of the binding of 200 ng/ml of recombinant rat Jagged-1/Fc to immobilized recombinant rat Notch-1/Fc or recombinant rat Notch-2/Fc (100 µl of a 5 µg/ml solution coated in each well) in an ELISA assay. WB: Use at a concentration of 0.1 - 0.2 µg/ml, this concentration is determined using rat jagged-1 at 5 ng/lane under non-reducing conditions. Predicted molecular weight: 146 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Source/Host: Goat

Purity/Purification: Immunogen affinity purified

Clonality: Polyclonal

Crossreactivity: Human and Rat. Not yet tested in other species.

Storage: Aliquot and store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for JAGGED 1 ANTIBODY:

ELISA, FACS, Neut, WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

JAGGED 1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for Jagged 1 antibody: Jagged 1; JAGL1; Goat polyclonal to Jagged 1; Protein jagged-1

Jagged 1 is a notch ligand. The Notch signaling system regulates proliferation, differentiation and apoptosis in many tissues, including hematopoietic tissue. Notch is a family of transmembrane receptors activated by ligands such as jagged 1, jagged 2 and delta 1 that are expressed on adjacent cells. Hematopoietic stem cells and early progenitors express notch 1 and notch 2 whereas primary stromal cultures have been shown to express jagged 1, indicating that jagged 1 may play a role in nurturing progenitor cells within the hematopoietic microenvironment. Cells engineered to express high levels of jagged 1 caused a four-fold increase in formation of hematopoietic progenitor cell colonies. However, without the presence of other cytokines that stimulate proliferation and maturation, such as IL3, IL6, SCF and GCSF, jagged 1 actually decreased myeloid colony formation by CD34+ cells, suggesting that jagged 1 modulates cytokine signals and helps preserve CD34+ cells in an immature state.

Function: Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).

Subunit: Interacts with NOTCH1, NOTCH2 and NOTCH3 (By similarity).

Subcellular Location: Membrane; Single-pass type I membrane protein.

Tissue Specificity: Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.

Developmental Stage: Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.

Disease: Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.

Disease: Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.

Disease: The mutation Asp-274 is "leaky". Two populations of proteins are produced from this allele. One population is abnormally glycosylated and is retained intracellularly rather than being transported to the cell surface. A second population is normally glycosylated and is transported to the cell surface where it is able to signal to the Notch receptor. The Asp-274 protein is temperature sensitive, with more abnormally glycosylated (and nonfunctional) molecules produced at higher temperatures. Carriers of this mutation therefore have more than 50% but less than 100% of the normal concentration of molecules on the cell surface. The cardiac-specific phenotype associated with this mutation suggests that the developing heart is more sensitive than the developing liver to decreased dosage of JAG1 protein.

Similarity: Contains 1 DSL domain.

Similarity: Contains 15 EGF-like domains.

Jagged 1 reacts with human and rat. not yet tested in other species..

OMIM: 118450; phenotype. [NCBI / EBI]
187500; phenotype. [NCBI / EBI]
601920; gene+phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Notch signaling pathway 04330
Reactome Event:Signaling by Notch 157118

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: Jagged 1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of Jagged 1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about Jagged 1 antibody, all related information, articles, resources about Jagged 1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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