EPO receptor antibody, EPO receptor Antibody, Goat Polyclonal Antibody

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EPO receptor Antibody, Goat Polyclonal Antibody

Catalog Number: 18-272-195181

Buy EPO receptor antibody -

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Related Product Names:

- EPO receptor antibody; EPO receptor; 2057; EPOR; 133100; P19235; MGC138358;; EPO receptor
- Goat polyclonal to EPO Receptor; 2057; EPOR; 133100; P19235; MGC138358;; Erythropoietin receptor

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: EPO receptor	Gene Name Synonym: 2057; EPOR; 133100; P19235; MGC138358;
Gi #: N/A	NCBI Acc #: NP_000112.1	Swiss Prot Acc #: P19235
Length (aa): N/A	Mol. Weight (Da): 55065	Chrom Location: N/A

Isotype: IgG

Immunogen: Recombinant full length protein (Human), expressed in mouse myeloma NSO cells.

Antigen Species: Human

Target: EPO receptor

Localization: Plasma membrane

Concentration: 0.1 mg/ml

Storage Buffer: Preservative: NoneConstituents: PBS

Application Note: ELISA: Use at a concentration of 0.5 - 1 µg/ml. The detection limit is approximately 0.3 ng/well. WB: Use at a concentration of 0.1 - 2 µg/ml. Detects a band of approximately 50 kDa. The detection limit is approximately 5 ng/lane under non-reducing and reducing conditions. Endotoxin: < 10 ng/mg of antibody determined by the LAL method. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular Localization: Plasma membrane

Source/Host: Goat

Purity/Purification: Immunogen affinity purified

Clonality: Polyclonal

Crossreactivity: Human - Not yet tested in other species

Storage: Aliquot and store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for EPO RECEPTOR ANTIBODY:

ELISA, WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

EPO RECEPTOR ANTIBODY TARGET DESCRIPTION:

Synonym Names for EPO receptor antibody: EPO receptor; 2057; EPOR; 133100; P19235; MGC138358;; Goat polyclonal to EPO Receptor; 2057; EPOR; 133100; P19235; MGC138358;; Erythropoietin receptor

Recombinant Human Erythropoietin Receptor (EPO R) is a type I membrane-spanning protein, expressed in a mouse myeloma cell line, NSO. This product is produced from a cDNA sequence encoding the extracellular domain of the EPO receptor. The 225 amino acid residue mature recombinant soluble EPO receptor, generated after cleavage of a 25 amino acid residue signal peptide, has predicted molecular mass of approximately 25 kDa. From glycosylation, recombinant human EPO receptor migrates as an approximately 32 kDa protein in SDS-PAGE.Erythropoietin (EPO), a glycoprotein produced primarily by the kidney and at lower levels by the liver, is the primary regulatory factor of erythropoiesis. It promotes the proliferation, differentiation, and survival of the erythroid progenitors. The biological effects of EPO are mediated by the erythropoietin receptor (EPO R). The EPO receptor belongs to the cytokine receptor superfamily, which includes the receptors for granulocyte-macrophage colony-stimulating factor (GMCSF), granulocyte colony-stimulating factor (G-CSF), IL2, IL3, IL4, IL5, IL6, IL7, LIF, and others.These receptors contain four conserved cysteines and a Trp-Ser-X-Trp-Ser (WSXWS) motif in their extracellular domains where X represents any amino acid. The EPO receptor differs from the other members of the cytokine receptor superfamily in that the EPO receptor can be stimulated to signal cell growth by binding either EPO or gp55. Human and mouse EPO receptor share 82 % identity in their amino acid sequence. Truncated and soluble forms of the EPO receptor result from alternative splicing of the EPO receptor gene. The gene for human EPO receptor has been mapped to the p region of chromosome 19. Recombinant EPO receptor binds EPO with high affinity and is a potent EPO antagonist. When EPO is present at low concentrations, the EPO receptor initiates prolongation of G1 of the cell cycle and sends a differentiation signal; whereas at high EPO concentrations, a proliferation signal is generated and the G1 is shortened. EPO binding induces the stimulation of Jak2 tyrosine kinase, which leads to tyrosine phosphorylation of several proteins, including the EPO receptor, resulting in the activation of intracellular pathways (Ras/MAP kinase, phosphatidylinositol 3-kinase, and STAT transcription factors). Recombinant Human Erythropoietin Receptor is expressed in megakaryocytes, erythoid progenitors, endothelial cells, and, possibly, neurons. It has also been found in human sera. Expression of the EPO receptor in normally IL-3-dependent cell lines such as Ba/F3 (pro-B lymphocyte cell line) or 32D (myeloid progenitor cell line) allows cell proliferation in the presence of either EPO or IL3, indicating that the EPO receptor is capable of generating a proliferative signal in these cells.

Function: Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.

Function: Isoform EPOR-T, missing the cytoplasmic tail, acts as a dominant-negative receptor of EPOR-mediated signaling.

Subunit: Forms homodimers on EPO stimulation. The tyrosine-phosphorylated form interacts with several SH2 domain-containing proteins including LYN (By similarity), the adapter protein APS, PTPN6 (By similarity), PTPN11, JAK2, PI3 kinases, STAT5A/B, SOCS3, CRKL (By similarity). Interacts with INPP5D/SHIP1 (By similarity). The N-terminal SH2 domain of PTPN6 binds Tyr-454 and inhibits signaling through dephosphorylation of JAK2 (By similarity). APS binding also inhibits the JAK-STAT signaling. Binding to PTPN11, preferentially through the N-terminal SH2 domain, promotes mitogenesis and phosphorylation of PTPN11 (By similarity). Binding of JAK2 (through its N-terminal) promotes cell-surface expression (By similarity). Interaction with the ubiquitin ligase NOSIP mediates EPO-induced cell proliferation. Interacts with ATXN2L.

Subcellular Location: Cell membrane; Single-pass type I membrane protein.

Subcellular Location: Isoform EPOR-S: Secreted. Note=Secreted and located to the cell surface.

Tissue Specificity: Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.

Domain: The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

Domain: The box 1 motif is required for JAK interaction and/or activation.

Domain: Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

Ptm: On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.

Ptm: Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.

Disease: Defects in EPOR are the cause of erythrocytosis familial type 1 (ECYT1) [MIM:133100]. ECYT1 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.

Similarity: Belongs to the type I cytokine receptor family. Type 1 subfamily.

Similarity: Contains 1 fibronectin type-III domain.

EPO receptor reacts with human - not yet tested in other species.

OMIM: 133100; phenotype. [NCBI / EBI]
133171; gene. [NCBI / EBI]

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PRODUCT INTERACTIONS:

EPO receptor interacts with PLC-gamma2 (Phospho-Tyr753), IgG (GenWay Catalog #: 18-272-195181).

EPO receptor interacts with PLC-gamma2 (Ab-753), IgG (GenWay Catalog #: 18-272-195181).

EPO receptor interacts with SOCS2, IgG (GenWay Catalog #: 18-272-195181).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

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Precautions: EPO receptor antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of EPO receptor antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about EPO receptor antibody, all related information, articles, resources about EPO receptor antibody are being stored on our online database. Let us know if you have questions regarding this product.

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