RB1 antibody, RB1 phospho S780 Antibody, Rabbit Polyclonal Antibody

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RB1 phospho S780 Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-272-197345

Buy RB1 antibody -

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Related Product Names:

- RB1 antibody; RB1; 5925; RB1; 109800; P06400; OSRC; RB;; RB1 phospho S780
- Rabbit polyclonal to Rb ( phospho S780 ); 5925; RB1; 109800; P06400; OSRC; RB;; Retinoblastoma-associated protein

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: RB1	Gene Name Synonym: 5925; RB1; 109800; P06400; OSRC; RB;
Gi #: N/A	NCBI Acc #: NP_000312.2	Swiss Prot Acc #: P06400
Length (aa): N/A	Mol. Weight (Da): 106159	Chrom Location: N/A

Isotype: IgG

Immunogen: Synthetic peptide (Human) derived from a region of human Rb that contains serine 780 (based on Swiss Protein database, accession number P06400). The sequence is conserved in mouse and rat.

Antigen Species: Human

Positive Control: Jurkat cells in high growth phase

Target: RB1

Localization: Nuclear

Concentration: 0.5 mg/ml

Purification Note: Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Rb protein, and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phosphoserine, irrespective of the sequence. The final product is generated by affinity chromatography using a Rb derived peptide that is phosphorylated at serine 780.

Storage Buffer: PBS pH 7.3 (without Mg2+ and Ca2+) containing 50% glycerol, 1.0mg/ml BSA (IgG, protease free). and 0.05% sodium azide.

Application Note: For WB: Use at a concentration of 1:900 - 1:1,100µg/ml. Predicted molecular weight: 106 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.

Target protein molecular weight: 120kD

Cellular Localization: Nuclear

Source/Host: Rabbit

Purity/Purification: Affinity purified with antigen

Clonality: Polyclonal

Crossreactivity: Human, Mouse, Rat - Not tested in other species - Expected to cross-react with Chicken (93% identity with immunogen).

Format: Liquid

Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for RB1 ANTIBODY:

WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

RB1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for RB1 antibody: RB1; 5925; RB1; 109800; P06400; OSRC; RB;; Rabbit polyclonal to Rb ( phospho S780 ); 5925; RB1; 109800; P06400; OSRC; RB;; Retinoblastoma-associated protein

Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F 1, PU 1, ATF 2, UBF, Elf 1 and cAbl. This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus 16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Function: Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Subunit: Interacts with ATAD5 (By similarity). The hypophosphorylated form interacts with and sequesters the E2F1 transcription factor. The unphosphorylated form interacts with ARID3B, JARID1A, SUV39H1, MJD2A/JHDM3A and THOC1. Interacts with the N-terminal domain of TAF1. Interacts with AATF, DNMT1, LIN9, LMNA, SUV420H1, SUV420H2, PELP1 and TMPO-alpha. May interact with NDC80. Interacts with EID1 and UBR4. Interacts with ARID4A and JARID1B. Interacts with E4F1. Interacts with adenovirus E1A protein, HPV E7 protein and SV40 large T antigen.

Subcellular Location: Nucleus.

Tissue Specificity: Expressed in the retina.

Ptm: Phosphorylated in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb.

Disease: Defects in RB1 are the cause of childhood cancer retinoblastoma (RB) [MIM:180200]. RB is a congenital malignant tumor that arises from the nuclear layers of the retina. It occurs in about 1:20'000 live births and represents about 2% of childhood malignancies. It is bilateral in about 30% of cases. Although most RB appear sporadically, about 20% are transmitted as an autosomal dominant trait with incomplete penetrance. The diagnosis is usually made before the age of 2 years when strabismus or a gray to yellow reflex from pupil ("cat eye") is investigated.

Disease: Defects in RB1 are a cause of bladder cancer [MIM:109800].

Disease: Defects in RB1 are a cause of osteogenic sarcoma [MIM:259500].

Similarity: Belongs to the retinoblastoma protein (RB) family.

RB1 phospho S780 reacts with human, mouse, rat - not tested in other species - expected to cross-react with chicken (93% identity with immunogen)..

OMIM: 109800; phenotype. [NCBI / EBI]
180200; gene+phenotype. [NCBI / EBI]
259500; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Cell cycle 04110
KEGG pathway: Chronic myeloid leukemia 05220
KEGG pathway: Glioma 05214
KEGG pathway: Melanoma 05218
KEGG pathway: Non-small cell lung cancer 05223
KEGG pathway: Pancreatic cancer 05212
KEGG pathway: Prostate cancer 05215
KEGG pathway: Small cell lung cancer 05222
Reactome Event:Cell Cycle, Mitotic 69278
Reactome Event:DNA Replication 69306

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PRODUCT INTERACTIONS:

RB1 phospho S780 interacts with CDK2, IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with Cdk2, IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with CDK2 (Phospho-Thr160), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with CDK2 (Ab-160), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with CDK2 (Phospho-Thr160) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with CDK2, IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with Anti-cdk2, IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with E2F1 (E2F transcription factor 1), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with E2F1 [KH129], IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with E2F1 [6B5], IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with E2F1 [18E10], IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with E2F1 [10D1 2B5], IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with E2F1 [17E2], IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with E2F1 (E2F transcription factor 1), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with Anti-E2F-1 pS364 (Rabbit), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with E2F1, IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with E2F1 [18E10], IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with E2F1 [16G7], IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with E2F1 [10D1 2B5], IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with E2F1 [17E2], IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with HDAC1 (histone deacetylase 1), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with HDAC1 [HDAC1-21], IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with Anti-HDAC-1, IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with HDAC1, IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with HDAC1 (ready-to-use), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with HDAC1, IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with MORF4L2 (mortality factor 4 like 2), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with AIB3/NCOA6 (aa 22-146), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with AIB3/NCOA6 (aa 903-1029), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with AIB3/NCOA6 (aa 1954-2067), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with PRMT2 (protein arginine methyltransferase 2), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with TBP Associated Factor 1 [0.T.145], IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with TAF1 (TAF1 RNA polymerase II. TATA box binding protein (TBP)-associated factor. 250kDa), IgG (GenWay Catalog #: 18-272-197345).

RB1 phospho S780 interacts with Anti-Transcription Initiation Factor TFIID Subunit 1 (TAF1), IgG (GenWay Catalog #: 18-272-197345).

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Precautions: RB1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of RB1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about RB1 antibody, all related information, articles, resources about RB1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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