PARP cleaved antibody, PARP cleaved Antibody, Rabbit Polyclonal Antibody

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PARP cleaved Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-272-197373

Buy PARP cleaved antibody -

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Related Product Names:

- PARP cleaved antibody; PARP cleaved; 142; PARP1; 173870; P09874; ADPRT; ADPRT1; PARP; PARP-1; PPOL; pADPRT-1;; PARP cleaved
- Rabbit polyclonal to cleaved PARP; 142; PARP1; 173870; P09874; ADPRT; ADPRT1; PARP; PARP-1; PPOL; pADPRT-1;; Poly [ADP-ribose] polymerase 1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: PARP cleaved	Gene Name Synonym: 142; PARP1; 173870; P09874; ADPRT; ADPRT1; PARP; PARP-1; PPOL; pADPRT-1;
Gi #: N/A	NCBI Acc #: NP_001609.2	Swiss Prot Acc #: P09874
Length (aa): N/A	Mol. Weight (Da): 113084	Chrom Location: N/A

Isotype: IgG

Immunogen: Synthetic peptide (Human) corresponding to N-terminus of cleavage site (214/215) of human PARP.

Antigen Species: Human

Specificity: This antibody specifically recognizes the 85 kDa fragment of cleaved PARP and can be used as marker for detecting apoptotic cells.

Positive Control: HeLa cells treated with staurosporine at 0.5 µM for 5 hours or bovine PARP cleaved by caspase 3

Target: PARP cleaved

Localization: Nuclear

Purification Note: Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a peptide spanning the cleavage site to remove antibody that is reactive with full length PARP. The final product is generated by affinity chromatography using a peptide corresponding to the PARP cleavage site.

Storage Buffer: Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.4

Application Note: ICC: Use at a dilution of 1/100. WB: Use at a dilution of 1/1000. Predicted molecular weight: 85 kDa. Can be blocked with Blocking peptide for PARP Cleavage site antibody Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular Localization: Nuclear

Source/Host: Rabbit

Purity/Purification: Immunogen affinity purified

Clonality: Polyclonal

Crossreactivity: Cross-reacts with Cow and Human. Not yet tested in other species.

Format: Liquid

Storage: Upon Receipt - Keep as concentrated solution. Aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for PARP CLEAVED ANTIBODY:

ICC, WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

PARP CLEAVED ANTIBODY TARGET DESCRIPTION:

Synonym Names for PARP cleaved antibody: PARP cleaved; 142; PARP1; 173870; P09874; ADPRT; ADPRT1; PARP; PARP-1; PPOL; pADPRT-1;; Rabbit polyclonal to cleaved PARP; 142; PARP1; 173870; P09874; ADPRT; ADPRT1; PARP; PARP-1; PPOL; pADPRT-1;; Poly [ADP-ribose] polymerase 1

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, is a highly conserved nuclear enzyme implicated in DNA repair and in the apoptosis response of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARPs N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.

Function: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks.

Catalytic Activity: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.

Subunit: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity).

Subcellular Location: Nucleus.

Ptm: Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.

Ptm: Poly-ADP-ribosylated by PARP2.

Miscellaneous: The ADP-D-ribosyl group of NAD(+) is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units.

Similarity: Contains 1 BRCT domain.

Similarity: Contains 1 PARP alpha-helical domain.

Similarity: Contains 1 PARP catalytic domain.

Similarity: Contains 2 PARP-type zinc fingers.

PARP cleaved reacts with cross-reacts with cow and human. not yet tested in other species..

OMIM: 173870; gene. [NCBI / EBI]

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PRODUCT INTERACTIONS:

PARP cleaved interacts with Aprataxin Isoform D, Protein (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with CEBP-alpha, Protein (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with CEBP Alpha, Protein (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with CEBPA [15C8], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with CEBPA (CCAAT/enhancer binding protein (C/EBP). alpha), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with CEBPA, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with E2F1 (E2F transcription factor 1), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with E2F1 [KH129], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with E2F1 [6B5], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with E2F1 [18E10], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with E2F1 [10D1 2B5], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with E2F1 [17E2], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with E2F1 (E2F transcription factor 1), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with Anti-E2F-1 pS364 (Rabbit), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with E2F1, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with E2F1 [18E10], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with E2F1 [16G7], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with E2F1 [10D1 2B5], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with E2F1 [17E2], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with Cellular tumor antigen p53, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 Human, Protein (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53M Human, Protein (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [SP5], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 phospho S15, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [DO-13], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [DO-7], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [Pab240], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [D01], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [3F301], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [DO-7] (ready-to-use), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [BP53-12], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with I-kappaB-alpha (Phospho-Ser32/Ser36), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 (Phospho-Ser15), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 (Phospho-Ser46), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 (Phospho-Ser315), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 (Ab-15), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 (Ab-46), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 (Ab-315), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 (Phospho-Ser15) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 (Phospho-Ser46) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 (Phospho-Ser315) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with TP53 (tumor protein p53 (Li-Fraumeni syndrome)), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 (a.a. 32-79), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 (a.a. 88-109), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 (a.a. 306-393), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with Mouse p53, Wild Type, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with Human p53 Oncogene, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [B20.1 (BP 53.122)] antibody (ready-to-use), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 AIP1, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [BP53-12] (FITC), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [DO-2], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [DO-1], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with P53, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [B20.1 (BP 53.122)] antibody (FITC), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with P53 [SP5] (ready-to-use), IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [DO-1], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [B449], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [Pab240], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [Pab1801], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [D01], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53 [DO-2], IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with p53, IgG (GenWay Catalog #: 18-272-197373).

PARP cleaved interacts with WRN, IgG (GenWay Catalog #: 18-272-197373).

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Precautions: PARP cleaved antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of PARP cleaved antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about PARP cleaved antibody, all related information, articles, resources about PARP cleaved antibody are being stored on our online database. Let us know if you have questions regarding this product.

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