Lck antibody, Lck phospho Y192 Antibody, Rabbit Polyclonal Antibody

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Lck phospho Y192 Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-272-197396

Buy Lck antibody -

Volume:

Related Product Names:

- Lck antibody; Lck; Lck phospho Y192
- Rabbit polyclonal to Lck ( phospho Y192 ); Proto-oncogene tyrosine-protein kinase LCK

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: Lck	Gene Name Synonym: N/A
Gi #: N/A	NCBI Acc #: NP_001036236.1	Swiss Prot Acc #: P06239
Length (aa): N/A	Mol. Weight (Da): 58001	Chrom Location: N/A

Isotype: IgG

Immunogen: Synthetic peptide (Human) derived from the region of human Lck that contains tyrosine 192 (based on Swiss Protein database, accession number P06239). The sequence is conserved in human and mouse.

Antigen Species: Human

Specificity: Lck (79% homologous), Hck (79% homologous) and Lyn (93% homologous) have not been tested.

Positive Control: Full length untagged recombinant human Lck protein

Target: Lck

Localization: Cytoplasmic

Purification Note: Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Lck, and (ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine (irrespective of the sequence). The final product is generated by affinity chromatography using a Lck-derived peptide that is phosphorylated at tyrosine 192.

Storage Buffer: Preservative: 0.05% Sodium Azide; Constituents: PBS, 50% glycerol, 1mg/ml BSA (IgG, protease free). pH 7.4

Application Note: WB: Use at a concentration of 0.1 - 1.0 µg/ml. Predicted molecular weight: 60 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular Localization: Cytoplasmic

Source/Host: Rabbit

Purity/Purification: Immunogen affinity purified

Clonality: Polyclonal

Crossreactivity: Cross-reacts with Human. Expected to cross-react with Chicken (79% identity with immunogen), Mouse (100% identity with immunogen) due to sequence homology. Not yet tested in other species.

Storage: Aliquot and store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for LCK ANTIBODY:

WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

LCK ANTIBODY TARGET DESCRIPTION:

Synonym Names for Lck antibody: Lck; Rabbit polyclonal to Lck ( phospho Y192 ); Proto-oncogene tyrosine-protein kinase LCK

Lck (p56lck), a member of the Src family of non-receptor tyrosine protein kinases, is expressed predominantly in T cells. Lck function is critical both for T cell development in the thymus and activation of mature T cells in the periphery by antigen. The activity of Lck is regulated by phosphorylation of multiple sites, including the two conserved tyrosine residues, Tyr-505 (equivalent to Tyr-529 in c-Src) and Tyr-394 (equivalent to Tyr-418 in c-Src). Phosphorylation of Lck Tyr-192 blocks high affinity binding of other proteins to the SH2 domain of Lck, thereby negatively regulating its participation in T cell antigen receptor signalling.

Function: Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR.

Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Enzyme Regulation: Inhibited by tyrosine phosphorylation.

Subunit: Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDC2, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with SQSTM1. Interacts with phosphorylated LIME1. Interacts with CBLB and PTPRH.

Subcellular Location: Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Present in lipid rafts in an unactive form.

Tissue Specificity: Expressed specifically in lymphoid cells.

Domain: The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.

Ptm: Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.

Mass Spectrometry: Mass=57869.42; Method=MALDI; Range=2-509; Source=PubMed:11840567;.

Disease: A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.

Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

Similarity: Contains 1 protein kinase domain.

Similarity: Contains 1 SH2 domain.

Similarity: Contains 1 SH3 domain.

Lck phospho Y192 reacts with cross-reacts with human. expected to cross-react with chicken (79% identity with immunogen), mouse (100% identity with immunogen) due to sequence homology. not yet tested in other species..

OMIM: 153390; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Natural killer cell mediated cytotoxicity 04650
KEGG pathway: T cell receptor signaling pathway 04660
Reactome Event:HIV Infection 162906
Reactome Event:Signaling in Immune system 168256

Products similar to Lck antibody:

IgG

Lck [LCK-01], IgG

CLCNKB (chloride channel Kb), IgG

PRODUCT INTERACTIONS:

Lck phospho Y192 interacts with CD247, IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with CD3 zeta, IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with TCR zeta chain [G3] (FITC), IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with CD247, IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with TCR zeta chain [G3], IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with CD247, IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with KH domain-containing, RNA-binding, signal transduction-associated protein 1, IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with LAT, IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with LAT [LAT-01], IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with LAT phospho Y132, IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with LAT phospho Y191, IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with Tyrosine-protein kinase ZAP-70, Protein (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with ZAP 70, IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with Zap-70 (Phospho-Tyr319), IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with Zap-70 (Phospho-Tyr493), IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with Zap-70 (Ab-319), IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with Zap-70 (Ab-493), IgG (GenWay Catalog #: 18-272-197396).

Lck phospho Y192 interacts with ZAP 70, IgG (GenWay Catalog #: 18-272-197396).

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Precautions: Lck antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of Lck antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about Lck antibody, all related information, articles, resources about Lck antibody are being stored on our online database. Let us know if you have questions regarding this product.

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