Smad2 antibody, Smad2 phospho T8 Antibody, Rabbit Polyclonal Antibody

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Smad2 phospho T8 Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-272-197423

Buy Smad2 antibody -

Volume:

Related Product Names:

- Smad2 antibody; Smad2; 4087; SMAD2; 601366; Q15796; JV18; JV18-1; MADH2; MADR2; MGC22139; MGC34440; hMAD-2; hSMAD2;; Smad2 phospho T8
- Rabbit polyclonal to Smad2 ( phospho T8 ); 4087; SMAD2; 601366; Q15796; JV18; JV18-1; MADH2; MADR2; MGC22139; MGC34440; hMAD-2; hSMAD2;; Mothers against decapentaplegic homolog 2

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: Smad2	Gene Name Synonym: 4087; SMAD2; 601366; Q15796; JV18; JV18-1; MADH2; MADR2; MGC22139; MGC34440; hMAD-2; hSMAD2;
Gi #: N/A	NCBI Acc #: NP_001003652.1	Swiss Prot Acc #: Q15796
Length (aa): N/A	Mol. Weight (Da): 52306	Chrom Location: N/A

Isotype: IgG

Immunogen: Synthetic phospho peptide (Human) that contains threonine 8.The sequence is conserved in mouse and rat.

Antigen Species: Human

Specificity: SMAD 3 (92% homologous) has not been tested, but cross-reactivity may occur in cell systems expressing a high level of SMAD 3.

Positive Control: HepG2 cells treated with TGF

Target: Smad2

Localization: Cytoplasmic and Nuclear

Concentration: 0.35 mg/ml

Purification Note: The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated SMAD 2. The final product is generated by affinity chromatography using a SMAD 2 derived peptide that is phosphorylated at threonine 8.

Storage Buffer: Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3

Application Note: WB: Use at a concentration of 0.1 - 0.75 µg/ml. Detects a band of approximately 58 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Cellular Localization: Cytoplasmic and Nuclear

Source/Host: Rabbit

Purity/Purification: Immunogen affinity purified

Clonality: Polyclonal

Crossreactivity: Cross-reacts with Human. Expected to cross-react with Mouse (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species.

Storage: Aliquot and store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for SMAD2 ANTIBODY:

WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

SMAD2 ANTIBODY TARGET DESCRIPTION:

Synonym Names for Smad2 antibody: Smad2; 4087; SMAD2; 601366; Q15796; JV18; JV18-1; MADH2; MADR2; MGC22139; MGC34440; hMAD-2; hSMAD2;; Rabbit polyclonal to Smad2 ( phospho T8 ); 4087; SMAD2; 601366; Q15796; JV18; JV18-1; MADH2; MADR2; MGC22139; MGC34440; hMAD-2; hSMAD2;; Mothers against decapentaplegic homolog 2

SMAD 2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The SMAD family is divided into three subclasses: receptor-regulated SMAD's, activin/TGF‚ receptor-regulated (SMAD 2 and 3) or BMP receptor regulated (SMAD 1, 5, and 8); the common partner, (SMAD 4) that functions via its interaction to the various SMAD's; and the inhibitory SMAD's, (SMAD 6 and SMAD 7). SMAD 2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the SMAD's regulates SMAD 2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated SMAD 2 associates with SMAD 4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of SMAD 2 (as well as SMAD 3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances SMAD 2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of SMAD 2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for SMAD 2 mediated signaling.

Function: Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.

Subunit: Found in a complex with SMAD3 and TRIM33 upon addition of TGF-beta. Interacts with SMAD3 and TRIM33. Interacts with SARA (SMAD anchor for receptor activation); may form trimers with the SMAD4 co-SMAD. Interacts with FOXH1, homeobox protein TGIF, PEBP2-alpha subunit, CREB-binding protein (CBP), EP300 and SKI. Interacts with SNON; when phosphorylated at Ser-465/467. Interacts (via PY-motif) with SMURF2. Interacts with AIP1 and HGS. Interacts with NEDD4L in response to TGF-beta (By similarity). Interacts with LBXCOR1 and CORL2.

Subcellular Location: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.

Tissue Specificity: Expressed at high levels in skeletal muscle, heart and placenta.

Ptm: Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.

Ptm: In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation (By similarity).

Ptm: Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo.

Disease: Defects in SMAD2 are found in sporadic cases of colorectal carcinoma.

Similarity: Belongs to the dwarfin/SMAD family.

Similarity: Contains 1 MH1 (MAD homology 1) domain.

Similarity: Contains 1 MH2 (MAD homology 2) domain.

Smad2 phospho T8 reacts with cross-reacts with human. expected to cross-react with mouse (100% identity with immunogen) and rat (100% identity with immunogen) due to sequence homology. not yet tested in other species..

OMIM: 601366; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Adherens junction 04520
KEGG pathway: Cell cycle 04110
KEGG pathway: Colorectal cancer 05210
KEGG pathway: Pancreatic cancer 05212
KEGG pathway: TGF-beta signaling pathway 04350
KEGG pathway: Wnt signaling pathway 04310
Reactome Event:Signaling by TGF beta 170834

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Precautions: Smad2 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of Smad2 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

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