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| PKA alpha + beta phospho T197 Antibody, Rabbit Polyclonal Antibody |
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| Catalog Number: 18-272-197480 |
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| Related Product Names:
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PKA alpha + beta antibody; PKA alpha + beta; PKACA; PKA alpha + beta phospho T197
- Rabbit polyclonal to PKA alpha + beta ( catalytic subunits ) ( phospho T197 ); cAMP-dependent protein kinase catalytic subunit alpha |
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- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
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| Gene Name: PKA alpha + beta |
Gene Name Synonym: PKACA |
Gi #:
N/A |
NCBI Acc #:
NP_002721.1 |
Swiss Prot Acc #:
P17612 |
Length (aa): N/A |
Mol. Weight (Da): 40590 |
Chrom Location: N/A |
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 Isotype: IgG
Immunogen: Synthetic phosphopeptide derived from a region of human PKA that contains threonine 197.
Antigen Species: Human
Specificity: This antibody exibited a preference for PKA catalytic subunit beta in some tested cell lines.
Positive Control: Forskolin-treated NIH3T3 cells, and Y-1 mouse adrenal cortical cells
Target: PKA alpha + beta
Localization: Cytoplasmic and Nuclear
Concentration: 0.35 mg/ml
Purification Note: The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKA. The final product is generated by affinity chromatography using a PKA-derived peptide that is phosphorylated at threonine 197.
Storage Buffer: Preservative: 0.05% Sodium Azide; Constituents: PBS, 1mg/ml BSA. pH 7.3
Application Note: WB: Use at a concentration of 0.1 - 0.75 µg/ml. Detects a band of approximately 42 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Cellular Localization: Cytoplasmic and Nuclear
Source/Host: Rabbit
Purity/Purification: Immunogen affinity purified
Clonality: Polyclonal
Crossreactivity: Cross-reacts with Human and Mouse. Expected to cross-react with Pig (100% identity with immunogen), Cow (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species.
Format: Liquid
Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.
Shipping: Products may be shipped on ice pack or dry ice. |
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| APPLICATIONS for PKA ALPHA + BETA ANTIBODY: |
| WB: Tested |
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| PKA ALPHA + BETA ANTIBODY TARGET DESCRIPTION: |
Synonym Names for PKA alpha + beta antibody: PKA alpha + beta; PKACA; Rabbit polyclonal to PKA alpha + beta ( catalytic subunits ) ( phospho T197 ); cAMP-dependent protein kinase catalytic subunit alpha
c-AMP-dependent Protein Kinase (PKA) is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription. PKA is composed of conserved catalytic subunits and regulatory subunits that dissociate upon activation by cAMP. The catalytic subunit of PKA contains the activation loop and mediates DNA binding and substrate recognition. The catalytic subunit is assembled and expressed as an active form and is phosphorylated on threonine 197 by PDK-1 in the activation loop. Phosphorylation of threonine 197 is critical for PKA maturation, optimal conformation and catalytic activity and plays a key role in mediating its biological functions.
Function: Phosphorylates a large number of substrates in the cytoplasm and the nucleus.
Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.
Enzyme Regulation: Activated by cAMP.
Subunit: A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
Subcellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity).
Tissue Specificity: Isoform 2 is sperm specific.
Ptm: Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively (By similarity).
Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
Similarity: Contains 1 AGC-kinase C-terminal domain.
Similarity: Contains 1 protein kinase domain.
PKA alpha + beta phospho T197 reacts with cross-reacts with human and mouse. expected to cross-react with pig (100% identity with immunogen), cow (100% identity with immunogen) and rat (100% identity with immunogen) due to sequence homology. not yet tested in other species..
OMIM: 601639; gene. [NCBI / EBI]
Pathways:
KEGG pathway: Apoptosis 04210
KEGG pathway: Calcium signaling pathway 04020
KEGG pathway: Gap junction 04540
KEGG pathway: GnRH signaling pathway 04912
KEGG pathway: Hedgehog signaling pathway 04340
KEGG pathway: Insulin signaling pathway 04910
KEGG pathway: Long-term potentiation 04720
KEGG pathway: MAPK signaling pathway 04010
KEGG pathway: Melanogenesis 04916
KEGG pathway: Olfactory transduction 04740
KEGG pathway: Taste transduction 04742
KEGG pathway: Wnt signaling pathway 04310
Reactome Event:Integration of energy metabolism 163685
Reactome Event:Lipid and lipoprotein metabolism 73923
Products similar to PKA alpha + beta antibody:
Protein
 cAMP-dependent protein kinase, alpha-catalytic subunit, Protein
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| PRODUCT INTERACTIONS: |
PKA alpha + beta phospho T197 interacts with PKACa2- RIa2, Protein (GenWay Catalog #: 18-272-197480).
PKA alpha + beta phospho T197 interacts with PRKAR1A, Protein (GenWay Catalog #: 18-272-197480).
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SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: PKA alpha + beta antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of PKA alpha + beta antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about PKA alpha + beta antibody, all related information, articles, resources about PKA alpha + beta antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
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Thank you for visiting GenWay Biotech's Datasheet. GenWay is the world's leader in chicken, IgY antibody production, producing over 900 quality polyclonal IgY antibodies to date. From our unique recombinant protein production, utilizing a variety of protein expression systems, GenWay's polyclonal IgY antibodies incorporate more epitopes, increasing the success rate for ELISA, western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), and many more immunoassays. |
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