Lactate Dehydrogenase antibody, Lactate Dehydrogenase (HRP)*B1G1 Antibody, Goat Polyclonal Antibody

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Lactate Dehydrogenase (HRP)*B1G1 Antibody, Goat Polyclonal Antibody

Catalog Number: 18-272-197672

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Related Product Names:

- Lactate Dehydrogenase antibody; Lactate Dehydrogenase; Lactate Dehydrogenase (HRP)*B1G1
- Goat polyclonal to Lactate Dehydrogenase ( HRP ); L-lactate dehydrogenase B chain

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: Lactate Dehydrogenase	Gene Name Synonym: N/A
Gi #: N/A	NCBI Acc #: NP_002291.1	Swiss Prot Acc #: P07195
Length (aa): N/A	Mol. Weight (Da): 36638	Chrom Location: N/A

Isotype: IgG

Immunogen: Full length native Lactate Dehydrogenase (purified)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle]

Positive Control: WB: purified enzyme from rabbit muscle (Worthington Biochemicals)IHC-P: non-small-cell lung cancer (see Koukourakis et al)

Target: Lactate Dehydrogenase

Localization: Cytoplasmic

Conjugation: HRP

Conjugation Note: Horseradish Peroxidase (HRP)

Concentration: 10 mg/ml

Purification Note: From polyclonal serum

Storage Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin and 0.01% gentamicin sulfate

Application Note: Dot: Use at an assay dependent dilution. ELISA: 1:1,000 - 1:5,000. IHC-P: 1:200. Quench peroxidases with methanol and H2O2 3% for 15 min. Perform heat mediated antigen retrieval via the microwave method (3x5 min).before commencing with IHC staining protocol. Incubate antibody overnight. A modified streptavidin technique was used (Kwik kit) (see Koukourakis MI et al reference). IM: Use at an assay dependent dilution. WB: 1:1,000-1:10,000. Please note: The non-conjugated version of this antibody (18-272-197038) has been used in WB in the reference Zaman K et al (protocol available on the datasheet for 18-272-197038). Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Target protein molecular weight: 35kD

Cellular Localization: Cytoplasmic

Source/Host: Goat

Purity/Purification: IgG fraction

Clonality: Polyclonal

Crossreactivity: Reacts with Human, Mouse and Rabbit. Not yet tested in other species. Predicted to react with a wide range of species due to sequence homology.

Format: Liquid

Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for LACTATE DEHYDROGENASE ANTIBODY:

Dot, ELISA, IHC-Fr, IM, WB: Tested

TESTING: (secondary reagents and protocols )

Not Available

LACTATE DEHYDROGENASE ANTIBODY TARGET DESCRIPTION:

Synonym Names for Lactate Dehydrogenase antibody: Lactate Dehydrogenase; Goat polyclonal to Lactate Dehydrogenase ( HRP ); L-lactate dehydrogenase B chain

Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD).There are 5 different isoenzymes of LDH, LDH-1 to LDH-5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB.Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream).

Catalytic Activity: (S)-lactate + NAD+ = pyruvate + NADH.

Pathway: Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.

Subunit: Homotetramer.

Subcellular Location: Cytoplasm.

Disease: Defects in LDHB are a cause of hereditary LDHB deficiency [MIM:150100]. LDHB deficiency is usually asymptomatic.

Similarity: Belongs to the LDH/MDH superfamily. LDH family.

Lactate Dehydrogenase (HRP)*B1G1 reacts with reacts with human, mouse and rabbit. not yet tested in other species. predicted to react with a wide range of species due to sequence homology..

OMIM: 150100; gene+phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Cysteine metabolism 00272
KEGG pathway: Glycolysis / Gluconeogenesis 00010
KEGG pathway: Propanoate metabolism 00640
KEGG pathway: Pyruvate metabolism 00620

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SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

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Precautions: Lactate Dehydrogenase antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of Lactate Dehydrogenase antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about Lactate Dehydrogenase antibody, all related information, articles, resources about Lactate Dehydrogenase antibody are being stored on our online database. Let us know if you have questions regarding this product.

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