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| XRCC4 Antibody, Mouse Polyclonal Antibody |
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| Catalog Number: 18-272-198057 |
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| Related Product Names:
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XRCC4 antibody; XRCC4; XRCC, XRCC-4, ligase, lig, ku; XRCC4
- Mouse polyclonal antiserum to human XRCC4; 7518; XRCC4; 194363; Q13426;; 7518; XRCC4; 194363; Q13426; |
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- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
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| Gene Name: XRCC4 |
Gene Name Synonym: XRCC, XRCC-4, ligase, lig, ku |
Gi #:
N/A |
NCBI Acc #:
NP_003392.1 |
Swiss Prot Acc #:
Q13426 |
Length (aa): N/A |
Mol. Weight (Da): 38287 |
Chrom Location: N/A |
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 Isotype: IgG
Immunogen: Recombinant protein encoding full-length XRCC4 purified from E. coli.
Positive Control: HeLa
Target: XRCC4
Concentration: Antiserum
Purification Note: From ascitic fluid
Storage Buffer: Phosphate-buffered saline, pH 7.4, containing no preservatives
Application Note: For WB: Use at a dilution of 1:1, 000. Detects a band of approximately 42 kDa (predicted molecular weight: 38 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Target protein molecular weight: 46
Source/Host: Mouse
Purity/Purification: Mouse Polyclonal Antiserum
Clonality: Polyclonal
Crossreactivity: Human - Not yet tested in other species
Format: Liquid
Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.
Shipping: Products may be shipped on ice pack or dry ice. |
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| APPLICATIONS for XRCC4 ANTIBODY: |
| WB: Tested |
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| XRCC4 ANTIBODY TARGET DESCRIPTION: |
Synonym Names for XRCC4 antibody: XRCC4; XRCC, XRCC-4, ligase, lig, ku; Mouse polyclonal antiserum to human XRCC4; 7518; XRCC4; 194363; Q13426;; 7518; XRCC4; 194363; Q13426;
XRCC4 (X-ray cross complementation protein-4) is an essential component for DNA double strand break repair and V(D)J recombination. The XRCC4 gene encodes a 38kDa protein that is ubiquitously expressed and contains no sequence similarity to any previously described protein. Recent experiments have shown that XRCC4 directly interacts with DNA ligase IV implicating a possible role in DNA-end joining.
Function: Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Subunit: Homodimer and homotetramer in solution. The homodimer associates with LIG4, and the LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC. Seems to interact directly with PRKDC but not with the Ku p70/86 dimer. Interacts with XLF/Cernunnos. Interacts with APTX and APLF.
Subcellular Location: Nucleus.
Tissue Specificity: Widely expressed.
Ptm: Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
Ptm: Monoubiquitinated.
Ptm: Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination.
Similarity: Belongs to the XRCC4 family.
XRCC4 reacts with human - not yet tested in other species .
OMIM: 194363; gene. [NCBI / EBI]
Pathways:
Reactome Event:DNA Repair 73894
Reactome Event:HIV Infection 162906
Products similar to XRCC4 antibody:
IgG
 XRCC4, IgG
XRCC4, IgG
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| PRODUCT INTERACTIONS: |
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SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
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Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: XRCC4 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of XRCC4 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about XRCC4 antibody, all related information, articles, resources about XRCC4 antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
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