Ctnnb1 antibody, Beta-catenin Antibody, Rabbit Polyclonal Antibody

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Beta-catenin Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-662-20068

Buy Ctnnb1 antibody -

Volume:

Related Product Names:

- Ctnnb1 antibody; Ctnnb1; Catnb; Mesc; Beta-catenin
- Catenin (cadherin associated protein), beta 1, 88kDa [Mus musculus]

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: Ctnnb1	Gene Name Synonym: Catnb; Mesc
Gi #: 6671684	NCBI Acc #: NP_031640.1	Swiss Prot Acc #: Q02248
Length (aa): 781	Mol. Weight (Da): 85471	Chrom Location: N/A

Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser33,37 of human ß-catenin. The sequence of the immunogen is identical in human, mouse, rat and Xenopus.

Antibody Specificity: Specific for the ~83k beta-catenin phosphorylated at Ser33,37 in Western blots of human embryonic kidney cells. Recognizes human and rat forms of beta-catenin.

Source/Host: Rabbit

Purity/Purification: Affinity Purified

Clonality: Polyclonal

Crossreactivity: Human, mouse, rat, xenopus

Storage: 4 degree C for short term (weeks) and -20 degree C for long term. Avoid frequent freeze and thaw.

Stability: 6-12 months at -20 degree C.

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for CTNNB1 ANTIBODY:

WB: Tested

ELISA, ICC, IHC: Not Tested

TESTING: (secondary reagents and protocols )

Western Blot: Western Blots of human embryonic kidney cells. Recognizes human and rat forms of beta-catenin. ( anti-Ctnnb1 )

Ctnnb1 antibody Western Blot image
Ctnnb1

CTNNB1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for Ctnnb1 antibody: Ctnnb1; Catnb; Mesc; Catenin (cadherin associated protein), beta 1, 88kDa [Mus musculus]

Function: Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

Subunit: Two separate pools are found in the cytoplasm: one is PSEN1/cadherin/catenin complex which anchors to the actin cytoskeleton. The other pool is part of a large complex containing AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. Wnt-dependent activation of DVL antagonizes the action of GSK3B. When GSK3B activity is inhibited the complex dissociates, CTNNB1 is dephosphorylated and is no longer targeted for destruction. The stabilized protein translocates to the nucleus where it binds TCF/LEF-1 family members, TBP, BCL9 and possibly also RUVBL1 and DUPLIN. Binds CTNNBIP and EP300. CTNNB1 forms a ternary complex with LEF1 and EP300 that is disrupted by CTNNBIP1 binding (By similarity). Interacts with the PDZ domain of TAX1BP3; this interaction inhibits the transcriptional activity of CTNNB1 (By similarity). Interacts with BAIAP1, CARM1, CTNNA3, CXADR and PCDH11Y. Binds SLC9A3R1 (By similarity).

Subcellular Location: Cytoplasm. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Nucleus. Translocates to the nucleus when it is stabilized (low level of phosphorylation).

Ptm: Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-33 (By similarity).

Ptm: EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding (By similarity).

Ptm: Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X (Probable). Its ubiquitination leads to its subsequent proteasomal degradation (By similarity).

Similarity: Belongs to the beta-catenin family.

Similarity: Contains 12 ARM repeats.

Beta-catenin reacts with human, mouse, rat, xenopus.

Pathways:
KEGG pathway: Adherens junction 04520
KEGG pathway: Colorectal cancer 05210
KEGG pathway: Focal adhesion 04510
KEGG pathway: Leukocyte transendothelial migration 04670
KEGG pathway: Tight junction 04530
KEGG pathway: Wnt signaling pathway 04310

Products similar to Ctnnb1 antibody:

IgG

CTNNB1 (catenin (cadherin-associated protein). beta 1. 88kDa), IgG

beta Catenin [15B8], IgG

beta Catenin phospho S33 [BC-76], IgG

BETA-CATENIN, IgG

Catenin, beta, dephosphorylated (27-37), IgG

Peptide

beta-Catenin (Phospho-Ser33) Antibody Blocking Peptide, Peptide

beta-Catenin (Phospho-Thr41/Ser45) Antibody Blocking Peptide, Peptide

beta-Catenin (Phospho-Ser37) Antibody Blocking Peptide, Peptide

PRODUCT INTERACTIONS:

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for CTNNB1 ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Olson,L.E., Tollkuhn,J., Scafoglio,C., Krones,A., Zhang,J., Ohgi,K.A., Wu,W., Taketo,M.M., Kemler,R., Grosschedl,R., Rose,D.,, et al.
     Homeodomain-mediated beta-catenin-dependent switching events dictate cell-lineage determination
[2] Heiser,P.W., Lau,J., Taketo,M.M., Herrera,P.L. and Hebrok,M., et al.
     Stabilization of beta-catenin impacts pancreas growth
[3] Tobimatsu,T., Kaji,H., Sowa,H., Naito,J., Canaff,L., Hendy,G.N., Sugimoto,T. and Chihara,K.
     Parathyroid hormone increases beta-catenin levels through Smad3 in mouse osteoblastic cells
[4] Huang,Z., Xie,H., Ioannidis,V., Held,W., Clevers,H., Sadim,M.S. and Sun,Z.
     Transcriptional regulation of CD4 gene expression by T cell factor-1/beta-catenin pathway
[5] Cheon,S.S., Wei,Q., Gurung,A., Youn,A., Bright,T., Poon,R., Whetstone,H., Guha,A. and Alman,B.A.
     Beta-catenin regulates wound size and mediates the effect of TGF-beta in cutaneous healing
[6] , Butz S., Stappert J., Weissig H., Kemler R.
     Plakoglobin and beta-catenin: distinct but closely related.
[7] , Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., et al.
     The transcriptional landscape of the mammalian genome.
[8] , Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., et al.
     Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
[9] , Roose J., Molenaar M., Peterson J., Hurenkamp J., Brantjes H., Moerer P., van de Wetering M., Destree O., Clevers H.
     The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors.
[10] , Kikuchi A.
     Regulation of beta-catenin signaling in the Wnt pathway.

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Precautions: Ctnnb1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of Ctnnb1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about Ctnnb1 antibody, all related information, articles, resources about Ctnnb1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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