AKT1 antibody, Akt (Phospho-Thr308) Antibody, Rabbit Polyclonal Antibody

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Peptides \| Secondary Antibodies \| ELISA Kits

Akt (Phospho-Thr308) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-785-210011

Buy AKT1 antibody -

Size:

Related Product Names:

- AKT1 antibody; AKT1; PKB; RAC; Akt (Phospho-Thr308)
- EC 2.7.11.1; RAC-PK-alpha; Protein kinase B; PKB; C-AKT; RAC-alpha serine/threonine-protein kinase

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: AKT1	Gene Name Synonym: PKB; RAC
Gi #: N/A	NCBI Acc #: N/A	Swiss Prot Acc #: P31749
Length (aa): N/A	Mol. Weight (Da): 55686	Chrom Location: N/A

Binding Site: Thr308

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Akt around the prosphorylation site of threonine 308 (M-K-T^P-F-C).

Specificity/Sensitivity: Akt (phospho-Thr308) antibody detects endogenous levels of Akt only when phosphorylated at threonine 308.

WB Dilution: 1:500~1:1000

IHC Dilution: 1:50~1:100

IF Dilution: 1:100~1:200

Source/Host: Rabbit

Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation

Clonality: Polyclonal

Crossreactivity: Human, Mouse, Rat

Format: Rabbit IgG in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage: 4C for short term (weeks) and -20C for long term. Avoid frequent freeze and thaw.

Stability: 1 year at -20C

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for AKT1 ANTIBODY:

IHC, WB: Tested

TESTING: (secondary reagents and protocols )

Western Blot ( anti-AKT1 )

AKT1 antibody tern B image
AKT1

IHC ( anti-AKT1 )

AKT1 antibody image
AKT1

AKT1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for AKT1 antibody: AKT1; PKB; RAC; EC 2.7.11.1; RAC-PK-alpha; Protein kinase B; PKB; C-AKT; RAC-alpha serine/threonine-protein kinase

Function: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme Regulation: Three specific sites, one in the kinase domain (Thr-308) and the two other ones in the C-terminal regulatory region (Ser-473 and Tyr-474), need to be phosphorylated for its full activation.

Subunit: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN.

Subcellular Location: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).

Tissue Specificity: In all human cell types so far analyzed.

Domain: Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane.

Ptm: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).

Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.

Similarity: Contains 1 AGC-kinase C-terminal domain.

Similarity: Contains 1 PH domain.

Similarity: Contains 1 protein kinase domain.

Akt (Phospho-Thr308) reacts with human, mouse, rat.

OMIM: 164730; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Adipocytokine signaling pathway 04920
KEGG pathway: Apoptosis 04210
KEGG pathway: B cell receptor signaling pathway 04662
KEGG pathway: Chronic myeloid leukemia 05220
KEGG pathway: Colorectal cancer 05210
KEGG pathway: Endometrial cancer 05213
KEGG pathway: ErbB signaling pathway 04012
KEGG pathway: Fc epsilon RI signaling pathway 04664
KEGG pathway: Focal adhesion 04510
KEGG pathway: Glioma 05214
KEGG pathway: Insulin signaling pathway 04910
KEGG pathway: Jak-STAT signaling pathway 04630
KEGG pathway: MAPK signaling pathway 04010
KEGG pathway: Melanoma 05218
KEGG pathway: Non-small cell lung cancer 05223
KEGG pathway: Pancreatic cancer 05212
KEGG pathway: Prostate cancer 05215
KEGG pathway: Renal cell carcinoma 05211
KEGG pathway: Small cell lung cancer 05222
KEGG pathway: T cell receptor signaling pathway 04660
KEGG pathway: Tight junction 04530
KEGG pathway: Toll-like receptor signaling pathway 04620
KEGG pathway: VEGF signaling pathway 04370
KEGG pathway: mTOR signaling pathway 04150
Reactome Event:Apoptosis 109581

Products similar to AKT1 antibody:

IgG

NCOA3 [0.T.198], IgG

CD25 [MRC OX-39] (RPE), IgG

SMARCC1, IgG

PKB/Akt, C-terminal, IgG

AKT1 phospho S473 [104A282], IgG

Native_Protein

PKB (AKT) (460-480) Peptide, Native_Protein

Akt (Phospho-Thr308) Antibody Blocking Peptide, Peptide

Akt (Phospho-Ser473) Antibody Blocking Peptide, Peptide

RAC-alpha serine/threonine-protein kinase, Protein

PKAkt1/PKBa Human, Protein

PRODUCT INTERACTIONS:

Akt (Phospho-Thr308) interacts with B-cell lymphoma/leukemia 10, IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with Bcl-10, IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with Bcl10 [Carmen-1], IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with Bcl10, IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with Bcl10 [151], IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with Bcl10, IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with CDC37, IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with Glycogen synthase kinase-3 beta, Protein (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with GSK3-beta, IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with GSK3-beta (Phospho-Ser9), IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with GSK3-beta (Phospho-Tyr216), IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with GSK3-beta (Ab-9), IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with GSK3-beta (Ab-216), IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with GSK3-beta (Phospho-Ser9) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with Rabbit anti GSK3b pS9, IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with Phospho-Ser⁹ GSK3-beta, IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with Anti-GSK3 beta pS9, IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with ASK1, IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with ASK1 (Phospho-Ser83), IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with ASK1 (Phospho-Ser966), IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with ASK1 (Ab-83), IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with ASK1 (Ab-966), IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with ASK1 (Phospho-Ser83) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with ASK1 (Phospho-Ser966) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with Rabbit anti ASK-1 pS83, IgG (GenWay Catalog #: 18-785-210011).

Akt (Phospho-Thr308) interacts with Anti-ASK-1 phospho specific pS83, IgG (GenWay Catalog #: 18-785-210011).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for AKT1 ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Jones P.F., Jakubowicz T., Pitossi F.J., Maurer F., Hemmings B.A.
     Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily.
[2] Matsubara A., Wasson J.C., Donelan S.S., Welling C.M., Glaser B., Permutt M.A.
     Isolation and characterization of the human AKT1 gene, identification of 13 single nucleotide polymorphisms (SNPs), and their lack of association with Type II diabetes.
[3] Coffer P.J., Woodgett J.R.
     Molecular cloning and characterisation of a novel putative protein-serine kinase related to the cAMP-dependent and protein kinase C families.
[4] Coffer P.J., Woodgett J.R.
     Eur. J. Biochem. 205:1217-1218(1992).
[5] Delcommenne M., Tan C., Gray V., Rue L., Woodgett J.R., Dedhar S.
     Phosphoinositide-3-OH kinase-dependent regulation of glycogen synthase kinase 3 and protein kinase B/AKT by the integrin-linked kinase.
[6] Alessi D.R., Andjelkovic M., Caudwell F.B., Cron P., Morrice N., Cohen P., Hemmings B.A.
     Mechanism of activation of protein kinase B by insulin and IGF-1.
[7] Walker K.S., Deak M., Paterson A., Hudson K., Cohen P., Alessi D.R.
     Activation of protein kinase B beta and gamma isoforms by insulin in vivo and by 3-phosphoinositide-dependent protein kinase-1 in vitro: comparison with protein kinase B alpha.
[8] Kane S., Sano H., Liu S.C.H., Asara J.M., Lane W.S., Garner C.C., Lienhard G.E.
     A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain.
[9] Conus N.M., Hannan K.M., Cristiano B.E., Hemmings B.A., Pearson R.B.
     Direct identification of tyrosine 474 as a regulatory phosphorylation site for the Akt protein kinase.
[10] Ahn J.-Y., Rong R., Kroll T.G., Van Meir E.G., Snyder S.H., Ye K.
     PIKE (phosphatidylinositol 3-kinase enhancer)-A GTPase stimulates Akt activity and mediates cellular invasion.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: AKT1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of AKT1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about AKT1 antibody, all related information, articles, resources about AKT1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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