b-Catenin (CTNNB) antibody, b-Catenin (Ab-41/45) Antibody, Rabbit Polyclonal Antibody

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b-Catenin (Ab-41/45) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-785-210042

Buy b-Catenin (CTNNB) antibody -

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Related Product Names:

- b-Catenin (CTNNB) antibody; b-Catenin (CTNNB); CTNNB; b-Catenin (Ab-41/45)
- Beta-catenin; Catenin beta-1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: b-Catenin (CTNNB)	Gene Name Synonym: CTNNB
Gi #: N/A	NCBI Acc #: N/A	Swiss Prot Acc #: P35222
Length (aa): N/A	Mol. Weight (Da): 85497	Chrom Location: N/A

Binding Site: around Ser45

WB Dilution: 500~1000

IHC Dilution: 50~100

Source/Host: Rabbit

Purity/Purification: Affinity Purified

Clonality: Polyclonal

Crossreactivity: Human, Mouse, Rat

Format: In PBS, 0.02% sodium azide and 50% glycerol.

Storage: 4C for short term (weeks) and -20C for long term. Avoid frequent freeze and thaw.

Stability: 1 year at -20C

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for B-CATENIN (CTNNB) ANTIBODY:

WB: Tested

TESTING: (secondary reagents and protocols )

Western Blot ( anti-b-Catenin (CTNNB) )

b-Catenin (CTNNB) antibody tern B image

B-CATENIN (CTNNB) ANTIBODY TARGET DESCRIPTION:

Synonym Names for b-Catenin (CTNNB) antibody: b-Catenin (CTNNB); CTNNB; Beta-catenin; Catenin beta-1

Function: Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

Subunit: Two separate pools are found in the cytoplasm: one is PSEN1/cadherin/catenin complex which anchors to the actin cytoskeleton. The other pool is part of a large complex containing AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. Wnt-dependent activation of DVL antagonizes the action of GSK3B. When GSK3B activity is inhibited the complex dissociates, CTNNB1 is dephosphorylated and is no longer targeted for destruction. The stabilized protein translocates to the nucleus where it binds TCF/LEF-1 family members, TBP, BCL9 and possibly also RUVBL1 and DUPLIN. Binds CTNNBIP and EP300. CTNNB1 forms a ternary complex with LEF1 and EP300 that is disrupted by CTNNBIP1 binding (By similarity). Interacts with the PDZ domain of TAX1BP3; this interaction inhibits the transcriptional activity of CTNNB1 (By similarity). Interacts with AJAP1, BAIAP1, CARM1, CTNNA3, CXADR and PCDH11Y. Binds SLC9A3R1. Interacts with GLIS2. Interacts with SLC30A9 (By similarity).

Subcellular Location: Cytoplasm. Nucleus. Note=Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 promotes nuclear translocation.

Tissue Specificity: Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheats. Expressed in colon.

Ptm: Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.

Ptm: EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.

Ptm: Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1A, APC and TBL1X (Probable). Its ubiquitination leads to its subsequent proteasomal degradation.

Disease: Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.

Disease: Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.

Disease: Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.

Similarity: Belongs to the beta-catenin family.

Similarity: Contains 12 ARM repeats.

b-Catenin (Ab-41/45) reacts with human, mouse, rat.

OMIM: 116806; gene. [NCBI / EBI]
132600; phenotype. [NCBI / EBI]
155255; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Adherens junction 04520
KEGG pathway: Basal cell carcinoma 05217
KEGG pathway: Colorectal cancer 05210
KEGG pathway: Endometrial cancer 05213
KEGG pathway: Focal adhesion 04510
KEGG pathway: Leukocyte transendothelial migration 04670
KEGG pathway: Melanogenesis 04916
KEGG pathway: Pathogenic Escherichia coli infection - EHEC 05130
KEGG pathway: Pathogenic Escherichia coli infection - EPEC 05131
KEGG pathway: Prostate cancer 05215
KEGG pathway: Thyroid cancer 05216
KEGG pathway: Tight junction 04530
KEGG pathway: Wnt signaling pathway 04310
Reactome Event:Signaling by Wnt 195721

Products similar to b-Catenin (CTNNB) antibody:

IgG

beta Catenin phospho S33 [BC-76], IgG

BETA-CATENIN, IgG

Catenin, beta, dephosphorylated (27-37), IgG

Catenin, beta, dephosphorylated (35-50), IgG

Catenin beta, N-terminal (exon 2), IgG

Peptide

beta-Catenin (Phospho-Ser33) Antibody Blocking Peptide, Peptide

beta-Catenin (Phospho-Thr41/Ser45) Antibody Blocking Peptide, Peptide

beta-Catenin (Phospho-Ser37) Antibody Blocking Peptide, Peptide

PRODUCT INTERACTIONS:

b-Catenin (Ab-41/45) interacts with B-cell CLL/lymphoma 9 (BCL9), IgG (GenWay Catalog #: 18-785-210042).

b-Catenin (Ab-41/45) interacts with NDRG1 (N-myc downstream regulated gene 1), IgG (GenWay Catalog #: 18-785-210042).

b-Catenin (Ab-41/45) interacts with PRESENILIN-1, IgG (GenWay Catalog #: 18-785-210042).

b-Catenin (Ab-41/45) interacts with Presenilin 1 (ready-to-use), IgG (GenWay Catalog #: 18-785-210042).

b-Catenin (Ab-41/45) interacts with Rabbit anti Presenilin-1, IgG (GenWay Catalog #: 18-785-210042).

b-Catenin (Ab-41/45) interacts with Goat A' Presenilin-2 (N-terminal), IgG (GenWay Catalog #: 18-785-210042).

b-Catenin (Ab-41/45) interacts with Goat A' Presenilin-1 (N-terminal), IgG (GenWay Catalog #: 18-785-210042).

b-Catenin (Ab-41/45) interacts with Presenilin-1 N-terminal Peptide, Peptide (GenWay Catalog #: 18-785-210042).

b-Catenin (Ab-41/45) interacts with Presenilin 1, IgG (GenWay Catalog #: 18-785-210042).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for B-CATENIN (CTNNB) ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Huelsken J., Birchmeier W., Behrens J.
     E-cadherin and APC compete for the interaction with beta-catenin and the cytoskeleton.
[2] Zhang C., Yu Y., Zhang S., Wei H., Bi J., Zhou G., Dong C., Zai Y., Xu W., Gao F., et al.
     Functional prediction of the coding sequences of 75 new genes deduced by analysis of cDNA clones from human fetal liver.
[3] Livingston R.J., Rieder M.J., Chung M.-W., Ritchie T.K., Olson A.N., Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D., Schackwitz W.S., et al.
     NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).
[4] Kim J.-S., Crooks H., Dracheva T., Nishanian T.G., Singh B., Jen J., Waldman T.
     Oncogenic beta-catenin is required for bone morphogenetic protein 4 expression in human cancer cells.
[5] Shichijo S., Itoh K.
     Identification of immuno-peptidmics that are recognized by tumor-reactive CTL generated from TIL of colon cancer patients.
[6] Mueller T., Choidas A., Reichmann E., Ullrich A.
     Phosphorylation and free pool of beta-catenin are regulated by tyrosine kinases and tyrosine phosphatases during epithelial cell migration.
[7] Georgakopoulos A., Marambaud P., Efthimiopoulos S., Shioi J., Cui W., Li H.-C., Schutte M., Gordon R., Holstein G.R., Martinelli G., et al.
     Presenilin-1 forms complexes with the cadherin/catenin cell-cell adhesion system and is recruited to intercellular and synaptic contacts.
[8] von Kries J.P., Winbeck G., Asbrand C., Schwarz-Romond T., Sochnikova N., Dell'Oro A., Behrens J., Birchmeier W.
     Hot spots in beta-catenin for interactions with LEF-1, conductin and APC.
[9] Moreno-Bueno G., Gamallo C., Perez-Gallego L., Contreras F., Palacios J.
     Beta-catenin expression in pilomatrixomas. Relationship with beta-catenin gene mutations and comparison with beta-catenin expression in normal hair follicles.
[10] Tutter A.V., Fryer C.J., Jones K.A.
     Chromatin-specific regulation of LEF-1-beta-catenin transcription activation and inhibition in vitro.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: b-Catenin (CTNNB) antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of b-Catenin (CTNNB) antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about b-Catenin (CTNNB) antibody, all related information, articles, resources about b-Catenin (CTNNB) antibody are being stored on our online database. Let us know if you have questions regarding this product.

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