DNA-PK antibody, DNA PKcs (Ab-2609) Antibody, Rabbit Polyclonal Antibody

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Peptides \| Secondary Antibodies \| ELISA Kits

DNA PKcs (Ab-2609) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-785-210107

Buy DNA-PK antibody -

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Related Product Names:

- DNA-PK antibody; DNA-PK; HYRC; HYRC1; DNA PKcs (Ab-2609)
- EC 2.7.11.1; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460; DNA-dependent protein kinase catalytic subunit

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: DNA-PK	Gene Name Synonym: HYRC; HYRC1
Gi #: N/A	NCBI Acc #: N/A	Swiss Prot Acc #: P78527
Length (aa): N/A	Mol. Weight (Da): 469089	Chrom Location: N/A

Binding Site: around Thr2609

WB Dilution: 500~1000

IHC Dilution: 50~100

Source/Host: Rabbit

Purity/Purification: Affinity Purified

Clonality: Polyclonal

Crossreactivity: Human

Format: In PBS, 0.02% sodium azide and 50% glycerol.

Storage: 4C for short term (weeks) and -20C for long term. Avoid frequent freeze and thaw.

Stability: 1 year at -20C

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for DNA-PK ANTIBODY:

WB: Tested

TESTING: (secondary reagents and protocols )

Western Blot ( anti-DNA-PK )

DNA-PK antibody tern B image
DNA-PK

DNA-PK ANTIBODY TARGET DESCRIPTION:

Synonym Names for DNA-PK antibody: DNA-PK; HYRC; HYRC1; EC 2.7.11.1; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460; DNA-dependent protein kinase catalytic subunit

Function: Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, C1D, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, c-myc/MYC and RFA2.

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme Regulation: Inhibited by wortmannin.

Subunit: DNA-PK is a heterotrimer of PRKDC and the Ku p70-p86 (XRCC6-XRCC5) dimer. Formation of this complex may be promoted by interaction with ILF3. Associates with the DNA-bound Ku heterodimer, but it can also bind to and be activated by free DNA. Interacts with DNA-PKcs-interacting protein (KIP) with the region upstream the kinase domain. PRKDC alone also interacts with and phosphorylates DCLRE1C, thereby activating the latent endonuclease activity of this protein.

Subcellular Location: Nucleus.

Ptm: Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity: Belongs to the PI3/PI4-kinase family.

Similarity: Contains 1 FAT domain.

Similarity: Contains 1 FATC domain.

Similarity: Contains 2 HEAT repeats.

Similarity: Contains 1 PI3K/PI4K domain.

Similarity: Contains 3 TPR repeats.

DNA PKcs (Ab-2609) reacts with human.

OMIM: 600899; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Cell cycle 04110
Reactome Event:DNA Repair 73894

Products similar to DNA-PK antibody:

IgG

DNA PKcs (phospho-Thr2609), IgG

DNA PKcs, IgG

DNA PKcs [18-2], IgG

DNA PKcs, IgG

DNA PKcs (Phospho-Thr2609) Antibody Blocking Peptide, Peptide

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for DNA-PK ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Hartley K.O., Gell D., Smith G.C.M., Zhang H., Divecha N., Connelly M.A., Admon A., Lees-Miller S.P., Anderson C.W., Jackson S.P., et al.
     DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product.
[2] Gell D., Anderson C.W.
     Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases.
[3] Rieder M.J., Livingston R.J., Daniels M.R., Montoya M.A., Chung M.-W., Miyamoto K.E., Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D., et al.
     NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).
[4] Anderson C.W., Dunn J.J., Freimuth P.I., Galloway A.M., Allalunis-Turner M.J.
     Frameshift mutation in PRKDC, the gene for DNA-PKcs, in the DNA repair-defective, human, glioma-derived cell line M059J.
[5] Ladenburger E.M., Fackelmayer F.O., Hameister H., Knippers R.
     MCM4 and PRKDC, human genes encoding proteins MCM4 and DNA-PKcs, are close neighbours located on chromosome 8q12-->q13.
[6] Sipley J.D., Menninger J.C., Hartley K.O., Ward D.C., Jackson S.P., Anderson C.W.
     Gene for the catalytic subunit of the human DNA-activated protein kinase maps to the site of the XRCC7 gene on chromosome 8.
[7] Abe M.
     Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
[8] Poltoratsky V.P., Shi X., York J.D., Lieber M.R., Carter T.H.
     Human DNA-activated protein kinase (DNA-PK) is homologous to phosphatidylinositol kinases.
[9] Anderson C.W., Dunn J.J., Freimuth P.I.
     Sequence of the 3' segment (exons 70-86) of PRKDC, the gene for human DNA-PKcs.
[10] Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno F.R.
     Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.

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Precautions: DNA-PK antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of DNA-PK antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about DNA-PK antibody, all related information, articles, resources about DNA-PK antibody are being stored on our online database. Let us know if you have questions regarding this product.

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