NBS1/P95 antibody, p95/NBS1 (Ab-343) Antibody, Rabbit Polyclonal Antibody

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Peptides \| Secondary Antibodies \| ELISA Kits

p95/NBS1 (Ab-343) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-785-210297

Buy NBS1/P95 antibody -

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Related Product Names:

- NBS1/P95 antibody; NBS1/P95; NBS; NBS1; P95; p95/NBS1 (Ab-343)
- Nijmegen breakage syndrome protein 1; Cell cycle regulatory protein p95; Nibrin

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: NBS1/P95	Gene Name Synonym: NBS; NBS1; P95
Gi #: N/A	NCBI Acc #: N/A	Swiss Prot Acc #: O60934
Length (aa): N/A	Mol. Weight (Da): 84959	Chrom Location: N/A

Binding Site: around Ser343

WB Dilution: 500~1000

IHC Dilution: 50~100

Source/Host: Rabbit

Purity/Purification: Affinity Purified

Clonality: Polyclonal

Crossreactivity: Human

Format: In PBS, 0.02% sodium azide and 50% glycerol.

Storage: 4C for short term (weeks) and -20C for long term. Avoid frequent freeze and thaw.

Stability: 1 year at -20C

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for NBS1/P95 ANTIBODY:

WB: Tested

TESTING: (secondary reagents and protocols )

Western Blot ( anti-NBS1/P95 )

NBS1/P95 antibody tern B image

NBS1/P95 ANTIBODY TARGET DESCRIPTION:

Synonym Names for NBS1/P95 antibody: NBS1/P95; NBS; NBS1; P95; Nijmegen breakage syndrome protein 1; Cell cycle regulatory protein p95; Nibrin

Function: Component of the MRE11/RAD50/NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability.

Subunit: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50 and MRE11A (By similarity). Interacts with histone H2AFX this requires phosphorylation of H2AFX on 'Ser-139'. Interacts with TERF2 and with KPNA2.

Subcellular Location: Nucleus (By similarity). Note=Localizes to discrete nuclear foci after treatment with genotoxic agents (By similarity).

Tissue Specificity: Ubiquitous. Expressed at high levels in testis.

Domain: The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.

Domain: The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.

Domain: The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.

Ptm: Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.

Disease: Defects in NBN are the cause of Nijmegen breakage syndrome (NBS) [MIM:251260]. NBS is an autosomal recessive syndrome characterized by chromosomal instability, radiation sensitivity, microcephaly, growth retardation, immunodeficiency and predisposition to cancer, particularly to lymphoid malignancies.

Disease: Defects in NBN are a cause of genetic susceptibility to breast cancer (BC) [MIM:114480]. BC is an extremely common malignancy, affecting one in eight women during their lifetime. A positive family history has been identified as major contributor to risk of development of the disease, and this link is striking for early-onset breast cancer.

Disease: Defects in NBN may be associated with aplastic anemia [MIM:609135]. Aplastic anemia is a disease of bone-marrow failure characterized by peripheral pancytopenia and marrow hypoplasia. Most of the cases of aplastic anemia are idiopathic, some are familial and some are due to a viral infection or to exposure to chemicals and radiation.

Disease: Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).

Miscellaneous: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.

Similarity: Contains 1 BRCT domain.

Similarity: Contains 1 FHA domain.

p95/NBS1 (Ab-343) reacts with human.

OMIM: 114480; phenotype. [NCBI / EBI]
251260; phenotype. [NCBI / EBI]
602667; gene. [NCBI / EBI]
609135; phenotype. [NCBI / EBI]

Pathways:
Reactome Event:DNA Repair 73894

Products similar to NBS1/P95 antibody:

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AKAP8 / AKAP95, IgG

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p95/NBS1 (Phospho-Ser343), IgG

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Rabbit anti NALP2, IgG

Peptide

p95/NBS1 (Phospho-Ser343) Antibody Blocking Peptide, Peptide

PRODUCT INTERACTIONS:

p95/NBS1 (Ab-343) interacts with G1/S-specific cyclin-E1, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with CYCLIN E, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with Cyclin E (ready-to-use), IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with Cyclin E [HE12], IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with Rabbit anti Cyclin E, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with Cyclin E, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with HISTONE H2AX, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with PHOSPHORYLATED HISTONE H2AX (GAMMA-H2AX), IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with Histone H2A.X (Phospho-Ser139), IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with Histone H2A.X (Ab-139), IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with Histone H2A.X (Phospho-Ser139) Antibody Blocking Peptide, Peptide (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with H2A.X, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with H2A.X phospho S139, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with H2A.X, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with MDC1, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with Anti-MDC1, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with MDC, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with MRE11, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with Mre11, IgG (GenWay Catalog #: 18-785-210297).

p95/NBS1 (Ab-343) interacts with Mre11 [12D7], IgG (GenWay Catalog #: 18-785-210297).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for NBS1/P95 ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Varon R., Vissinga C., Platzer M., Cerosaletti K.M., Chrzanowska K.H., Saar K., Beckmann G., Seemanova E., Cooper P.R., Nowak N.J., et al.
     Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome.
[2] Carney J.P., Maser R.S., Olivares H., Davis E.M., Le Beau M., Yates J.R. III, Hays L., Morgan W.F., Petrini J.H.J.
     The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response.
[3] Matsuura S., Tauchi H., Nakamura A., Kondo N., Sakamoto S., Endo S., Smeets D., Solder B., Belohradsky B.H., Kaloustian V.M., et al.
     Positional cloning of the gene for Nijmegen breakage syndrome.
[4] Tauchi H., Matsuura S., Isomura M., Kinjo T., Nakamura A., Sakamoto S., Kondo N., Endo S., Komatsu K., Nakamura Y., et al.
     Sequence analysis of an 800-kb genomic DNA region on chromosome 8q21 that contains the Nijmegen breakage syndrome gene, NBS1.
[5] Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
     Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
[6] Livingston R.J., Rieder M.J., Chung M.-W., Ritchie T.K., Olson A.N., Nguyen C.P., Nguyen D.A., Poel C.L., Chambers S.W., Schackwitz W.S., et al.
     NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).
[7] Trujillo K.M., Yuan S.-S.F., Lee E.Y.-H.P., Sung P.
     Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95.
[8] Desai-Mehta A., Cerosaletti K.M., Concannon P.
     Distinct functional domains of nibrin mediate Mre11 binding, focus formation, and nuclear localization.
[9] Gatei M., Young D., Cerosaletti K.M., Desai-Mehta A., Spring K., Kozlov S., Lavin M.F., Gatti R.A., Concannon P., Khanna K.K., et al.
     ATM-dependent phosphorylation of nibrin in response to radiation exposure.
[10] Zhao S., Weng Y.-C., Yuan S.-S.F., Lin Y.-T., Hsu H.-C., Lin S.-C., Gerbino E., Song M.-H., Zdzienicka M.Z., Gatti R.A., et al.
     Functional link between ataxia-telangiectasia and Nijmegen breakage syndrome gene products.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: NBS1/P95 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of NBS1/P95 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about NBS1/P95 antibody, all related information, articles, resources about NBS1/P95 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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