P53 antibody, p53 (Phospho-Ser46) Antibody, Rabbit Polyclonal Antibody

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p53 (Phospho-Ser46) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-785-210332

Buy P53 antibody -

Size:

Related Product Names:

- P53 antibody; P53; P53; p53 (Phospho-Ser46)
- Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; Cellular tumor antigen p53

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: P53	Gene Name Synonym: P53
Gi #: N/A	NCBI Acc #: N/A	Swiss Prot Acc #: P04637
Length (aa): N/A	Mol. Weight (Da): 43653	Chrom Location: N/A

Binding Site: Ser46

WB Dilution: 500~1000

IHC Dilution: 50~100

Source/Host: Rabbit

Purity/Purification: Affinity Purified

Clonality: Polyclonal

Crossreactivity: Human

Format: In PBS, 0.02% sodium azide and 50% glycerol.

Storage: 4C for short term (weeks) and -20C for long term. Avoid frequent freeze and thaw.

Stability: 1 year at -20C

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for P53 ANTIBODY:

WB: Tested

TESTING: (secondary reagents and protocols )

Western Blot ( anti-P53 )

P53 antibody tern B image
P53

P53 ANTIBODY TARGET DESCRIPTION:

Synonym Names for P53 antibody: P53; P53; Tumor suppressor p53; Phosphoprotein p53; Antigen NY-CO-13; Cellular tumor antigen p53

Function: Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression.

Cofactor: Binds 1 zinc ion per subunit.

Subunit: Interacts with AXIN1. Probably part of a complex consisting of TP53, HIPK2 and AXIN1 (By similarity). Binds DNA as a homotetramer. Interacts with histone acetyltransferases EP300 and methyltransferases HRMT1L2 and CARM1, and recruits them to promoters. In vitro, the interaction of TP53 with cancer-associated/HPV (E6) viral proteins leads to ubiquitination and degradation of TP53 giving a possible model for cell growth regulation. This complex formation requires an additional factor, E6-AP, which stably associates with TP53 in the presence of E6. C-terminus interacts with TAF1, when TAF1 is part of the TFIID complex. Interacts with ING4 and this interaction may be indirect. Found in a complex with CABLES1 and TP73. Interacts with HIPK1, HIPK2, and P53DINP1. Interacts with WWOX. May interacts with HCV core protein. Interacts with USP7 and SYVN1. Interacts with HSP90AB1 (By similarity). Interacts with BANP.

Subcellular Location: Cytoplasm. Nucleus. Endoplasmic reticulum. Note=Interaction with BANP promotes nuclear localization.

Domain: The nuclear export signal acts as a transcriptional repression domain.

Ptm: Acetylated. Acetylation of Lys-382 by CREBBP enhances transcriptional activity. Deacetylation of Lys-382 by SIRT1 impairs its ability to induce proapoptotic program and modulate cell senescence.

Ptm: Phosphorylation on Ser residues mediates transcriptional activation. Phosphorylated by HIPK1 (By similarity). Phosphorylated on Thr-18 by VRK1, which may prevent the interaction with MDM2. Phosphorylated on Thr-55 by TAF1, which promotes MDM2-mediated degradation. Phosphorylated on Ser-46 by HIPK2 upon UV irradiation. Phosphorylation on Ser-46 is required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated on Ser-15 upon ultraviolet irradiation; which is enhanced by interaction with BANP.

Ptm: Dephosphorylated by PP2A. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A.

Ptm: May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line.

Ptm: Ubiquitinated by SYVN1, which leads to proteasomal degradation.

Disease: TP53 is found in increased amounts in a wide variety of transformed cells. TP53 is frequently mutated or inactivated in about 60% of cancers.

Disease: Defects in TP53 are involved in esophageal squamous cell carcinoma (ESCC) [MIM:133239]. ESCC is a tumor of the esophagus.

Disease: Defects in TP53 are a cause of Li-Fraumeni syndrome (LFS) [MIM:151623]. LFS is an autosomal dominant familial cancer syndrome that in its classic form is defined by the existence of both a proband with a sarcoma and two other first-degree relatives with a cancer by age 45 years. In these families the affected relatives develop a diverse set of malignancies at unusually early ages. The spectrum of cancers in LFS includes breast carcinomas, soft-tissue sarcomas, brain tumors, osteosarcoma, leukemia and adreno-cortical carcinoma. Other possible component tumors of LFS are melanoma, gonadal cell tumors and carcinomas of the lung, pancreas and prostate.

Disease: Defects in TP53 may be associated with nasopharyngeal carcinoma [MIM:161550]; also known as nasopharyngeal cancer.

Disease: Defects in TP53 are found in Barrett metaplasia; also known as Barrett esophagus. It is a condition in which the normally stratified squamous epithelium of the lower esophagus is replaced by a metaplastic columnar epithelium. The condition develops as a complication in approximately 10% of patients with chronic gastroesophageal reflux disease and predisposes to the development of esophageal adenocarcinoma.

Disease: Defects in TP53 are involved in head and neck squamous cell carcinomas (HNSCC) [MIM:275355].

Disease: Defects in TP53 are involved in oral squamous cell carcinoma (OSCC). Cigarette smoke is a prime mutagenic agent in cancer of the aerodigestive tract.

Disease: Defects in TP53 are a cause of lung cancer [MIM:211980].

Disease: Defects in TP53 are a cause of choroid plexus papilloma [MIM:260500]. Choroid plexus papilloma is a slow-growing benign tumor of the choroid plexus that often invades the leptomeninges. In children it is usually in a lateral ventricle but in adults it is more often in the fourth ventricle. Hydrocephalus is common, either from obstruction or from tumor secretion of cerebrospinal fluid. If it undergoes malignant transformation it is called a choroid plexus carcinoma. Primary choroid plexus tumors are rare and usually occur in early childhood.

Similarity: Belongs to the p53 family.

p53 (Phospho-Ser46) reacts with human.

OMIM: 133239; phenotype. [NCBI / EBI]
151623; phenotype. [NCBI / EBI]
161550; phenotype. [NCBI / EBI]
191170; gene+phenotype. [NCBI / EBI]
211980; phenotype. [NCBI / EBI]
260500; phenotype. [NCBI / EBI]
275355; phenotype. [NCBI / EBI]

Pathways:
KEGG pathway: Amyotrophic lateral sclerosis (ALS) 05030
KEGG pathway: Apoptosis 04210
KEGG pathway: Basal cell carcinoma 05217
KEGG pathway: Cell cycle 04110
KEGG pathway: Chronic myeloid leukemia 05220
KEGG pathway: Colorectal cancer 05210
KEGG pathway: Endometrial cancer 05213
KEGG pathway: Glioma 05214
KEGG pathway: Huntington's disease 05040
KEGG pathway: MAPK signaling pathway 04010
KEGG pathway: Melanoma 05218
KEGG pathway: Non-small cell lung cancer 05223
KEGG pathway: Pancreatic cancer 05212
KEGG pathway: Prostate cancer 05215
KEGG pathway: Small cell lung cancer 05222
KEGG pathway: Thyroid cancer 05216
KEGG pathway: Wnt signaling pathway 04310
Reactome Event:Cell Cycle Checkpoints 69620

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Peptide

p53 (Phospho-Ser315) Antibody Blocking Peptide, Peptide

p53 (Phospho-Ser46) Antibody Blocking Peptide, Peptide

p53 (Phospho-Ser15) Antibody Blocking Peptide, Peptide

p53 Human, Protein

p53M Human, Protein

PRODUCT INTERACTIONS:

p53 (Phospho-Ser46) interacts with ATR [2B5], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with ATR, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Cytoplasmic tyrosine-protein kinase BMX, Protein (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with CEBPB [1H7], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with CEBPB (CCAAT/enhancer binding protein (C/EBP). beta), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with NFIL6 [21A1], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with CREB BINDING PROTEIN, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with CBP, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with p300 [3G230], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with p300, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Huntingtin, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Huntingtin (a.a. 1844-2131), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Huntingtin (a.a. 2703-2911), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Huntingtin (a.a. 997-1276), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Anti-Huntington pS421, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with HUNTINGTIN, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with HDAC1 (histone deacetylase 1), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with HDAC1 [HDAC1-21], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Anti-HDAC-1, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with HDAC1, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with HDAC1 (ready-to-use), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with HDAC1, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with HMGB1 [HAP46.5], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with HMGB1 Human, Protein (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Mitogen-activated protein kinase 11, Protein (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with MDM2 [2A10], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with MDM2 [SMP14], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Rabbit anti Mouse MDM2 pS185, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with MDM2 [HDM2-323], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with MDM2 [MD-219], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with MDM2 (ready-to-use), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with NFYA (nuclear transcription factor Y. alpha), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Anti-NF-Y (A subunit) (Rabbit), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with NFY, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Nucleophosmin [NA24], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Nucleophosmin [FC82291], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with PARP cleaved [194C1439], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with PARP [10H], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with PARP cleaved, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with PARP1 (poly (ADP-ribose) polymerase family. member 1), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with POLY(ADP-RIBOSE) POLYMERASE, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with PARP cleaved, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with PARP cleaved (FITC), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with PARP cleaved, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with PARP (ready-to-use), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with PIAS4 (protein inhibitor of activated STAT. 4), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with PAIAS gamma, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Rad51 [14B4], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Rad51 [13E4], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with Rad51 [14B4], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with replication protein A1, 70kDa (RPA1), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with RPA70, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with TATA box binding protein, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with TATA binding protein TBP [1TB18], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with TATA binding protein TBP [1TBP18], IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with TBP (TATA box binding protein), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with TATA-Binding Protein, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with TP53BP2 (tumor protein p53 binding protein. 2), IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with UBE3A, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with E6AP, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with UBE3A, IgG (GenWay Catalog #: 18-785-210332).

p53 (Phospho-Ser46) interacts with WRN, IgG (GenWay Catalog #: 18-785-210332).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for P53 ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Zakut-Houri R., Bienz-Tadmor B., Givol D., Oren M.
     Human p53 cellular tumor antigen: cDNA sequence and expression in COS cells.
[2] Lamb P., Crawford L.
     Characterization of the human p53 gene.
[3] Harlow E., Williamson N.M., Ralston R., Helfman D.M., Adams T.E.
     Molecular cloning and in vitro expression of a cDNA clone for human cellular tumor antigen p53.
[4] Harris N., Brill E., Shohat O., Prokocimer M., Wolf D., Arai N., Rotter V.
     Molecular basis for heterogeneity of the human p53 protein.
[5] Buchman V.L., Chumakov P.M., Ninkina N.N., Samarina O.P., Georgiev G.P.
     A variation in the structure of the protein-coding region of the human p53 gene.
[6] Matlashewski G., Lamb P., Pim D., Peacock J., Crawford L., Benchimol S.
     Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene.
[7] Farrell P.J., Allan G., Shanahan F., Vousden K.H., Crook T.
     p53 is frequently mutated in Burkitt's lymphoma cell lines.
[8] Allalunis-Turner M.J., Barron G.M., Day R.S. III, Dobler K.D., Mirzayans R.
     Isolation of two cell lines from a human malignant glioma specimen differing in sensitivity to radiation and chemotherapeutic drugs.
[9] Chang N.-S., Pratt N., Heath J., Schultz L., Sleve D., Carey G.B., Zevotek N.
     Hyaluronidase induction of a WW domain-containing oxidoreductase that enhances tumor necrosis factor cytotoxicity.
[10] Chumakov P.M., Almazov V.P., Jenkins J.R.
     Submitted (JUN-1991) to the EMBL/GenBank/DDBJ databases.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: P53 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of P53 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

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