PAK1 antibody, PAK1 (Phospho-Thr212) Antibody, Rabbit Polyclonal Antibody

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Peptides \| Secondary Antibodies \| ELISA Kits

PAK1 (Phospho-Thr212) Antibody, Rabbit Polyclonal Antibody

Catalog Number: 18-785-210349

Buy PAK1 antibody -

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Related Product Names:

- PAK1 antibody; PAK1; PAK1 (Phospho-Thr212)
- EC 2.7.11.1; p21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK; Serine/threonine-protein kinase PAK 1

- Gene Information - Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.

Gene Name: PAK1	Gene Name Synonym: N/A
Gi #: N/A	NCBI Acc #: N/A	Swiss Prot Acc #: Q13153
Length (aa): N/A	Mol. Weight (Da): 60647	Chrom Location: N/A

Binding Site: Thr212

WB Dilution: 500~1000

IHC Dilution: 50~100

Source/Host: Rabbit

Purity/Purification: Affinity Purified

Clonality: Polyclonal

Crossreactivity: Human, Mouse, Rat

Format: In PBS, 0.02% sodium azide and 50% glycerol.

Storage: 4C for short term (weeks) and -20C for long term. Avoid frequent freeze and thaw.

Stability: 1 year at -20C

Shipping: Products may be shipped on ice pack or dry ice.

APPLICATIONS for PAK1 ANTIBODY:

IHC, WB: Tested

TESTING: (secondary reagents and protocols )

Western Blot ( anti-PAK1 )

PAK1 antibody tern B image
PAK1

IHC ( anti-PAK1 )

PAK1 antibody image
PAK1

PAK1 ANTIBODY TARGET DESCRIPTION:

Synonym Names for PAK1 antibody: PAK1; EC 2.7.11.1; p21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK; Serine/threonine-protein kinase PAK 1

Function: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Enzyme Regulation: Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-423 and allows the kinase domain to adopt an active structure. Also activated by binding to GTP-bound CDC42, independent of the phosphorylation state of Thr-423. Phosphorylation of Thr-84 by OXSR1 inhibits this activation (By similarity).

Subunit: Homodimer in its autoinhibited state. Active as monomer. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1.

Subcellular Location: Cytoplasm. Cell junction, focal adhesion. Note=Recruited to focal adhesions upon activation.

Ptm: Autophosphorylated when activated by CDC42/p21 and RAC1.

Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Similarity: Contains 1 CRIB domain.

Similarity: Contains 1 protein kinase domain.

PAK1 (Phospho-Thr212) reacts with human, mouse, rat.

OMIM: 602590; gene. [NCBI / EBI]

Pathways:
KEGG pathway: Axon guidance 04360
KEGG pathway: Epithelial cell signaling in Helicobacter pylori infection 05120
KEGG pathway: ErbB signaling pathway 04012
KEGG pathway: Focal adhesion 04510
KEGG pathway: MAPK signaling pathway 04010
KEGG pathway: Natural killer cell mediated cytotoxicity 04650
KEGG pathway: Regulation of actin cytoskeleton 04810
KEGG pathway: Renal cell carcinoma 05211
KEGG pathway: T cell receptor signaling pathway 04660

Products similar to PAK1 antibody:

IgG

PAK1, IgG

PRODUCT INTERACTIONS:

PAK1 (Phospho-Thr212) interacts with Ras-related C3 botulinum toxin substrate 1, Protein (GenWay Catalog #: 18-785-210349).

PAK1 (Phospho-Thr212) interacts with RAC1 + CDC42 phospho S71, IgG (GenWay Catalog #: 18-785-210349).

PAK1 (Phospho-Thr212) interacts with RAC1 Human, Protein (GenWay Catalog #: 18-785-210349).

PAK1 (Phospho-Thr212) interacts with RAC1, Protein (GenWay Catalog #: 18-785-210349).

SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.

BACKGROUND REFERENCES for PAK1 ANTIBODY:

Background references for antibody target are not specific to GenWay products

[1] Brown J.L., Stowers L., Baer M., Trejo J., Coughlin S., Chant J.
     Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway.
[2] Sells M.A., Knaus U.G., Bagrodia S., Ambrose D.M., Bokoch G.M., Chernoff J.
     Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells.
[3] Reid T., Aspenstroem P., Bertoglio J.
     Human PAK1B.
[4] Manser E., Huang H.Y., Loo T.H., Chen X.Q., Dong J.M., Leung T., Lim L.
     Expression of constitutively active alpha-PAK reveals effects of the kinase on actin and focal complexes.
[5] Frost J.A., Khokhlatchev A., Stippec S., White M.A., Cobb M.H.
     Differential effects of PAK1-activating mutations reveal activity-dependent and -independent effects on cytoskeletal regulation.
[6] Zhao Z.S., Manser E., Chen X.Q., Chong C., Leung T., Lim L.
     A conserved negative regulatory region in alphaPAK: inhibition of PAK kinases reveals their morphological roles downstream of Cdc42 and Rac1.
[7] Zenke F.T., King C.C., Bohl B.P., Bokoch G.M.
     Identification of a central phosphorylation site in p21-activated kinase regulating autoinhibition and kinase activity.
[8] Parrini M.C., Lei M., Harrison S.C., Mayer B.J.
     Pak1 kinase homodimers are autoinhibited in trans and dissociated upon activation by Cdc42 and Rac1.
[9] Chen S., Yin X., Zhu X., Yan J., Ji S., Chen C., Cai M., Zhang S., Zong H., Hu Y., et al.
     The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis.
[10] Vadlamudi R.K., Barnes C.J., Rayala S., Li F., Balasenthil S., Marcus S., Goodson H.V., Sahin A.A., Kumar R.
     p21-activated kinase 1 regulates microtubule dynamics by phosphorylating tubulin cofactor B.

Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.

Precautions: PAK1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.

Important Notes: During shipment, small volumes of PAK1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.

Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about PAK1 antibody, all related information, articles, resources about PAK1 antibody are being stored on our online database. Let us know if you have questions regarding this product.

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