|
|
| Shc1 (Phospho-Tyr427) Antibody, Rabbit Polyclonal Antibody |
 |
|
|
 |
| Catalog Number: 18-785-210408 |
|
|
|
| Related Product Names:
|
-
Shc1 antibody; Shc1; SHC; SHCA; Shc1 (Phospho-Tyr427)
- SH2 domain protein C1; Src homology 2 domain-containing-transforming protein C1; SHC-transforming protein 1 |
|
- Gene Information -
Information in yellow represents specific gene information and does not necessarily represent specific product details. For more information please contact sales@genwaybio.com.
|
|
| Gene Name: Shc1 |
Gene Name Synonym: SHC; SHCA |
Gi #:
N/A |
NCBI Acc #:
N/A |
Swiss Prot Acc #:
P29353 |
Length (aa): N/A |
Mol. Weight (Da): 62852 |
Chrom Location: N/A |
|
|
 Binding Site: Tyr427
Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Shc1 around the phosphorylation site of tyrosine 427 (P-S-YP-V-N).
WB Dilution: 500~1000
IHC Dilution: 50~100
Specificity/Sensitivity: Shc1 (Phospho-Tyr427) antibody detects endogenous levels of Shc1 only when phosphorylated at tyrosine 427.
Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Source/Host: Rabbit
Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation
Clonality: Polyclonal
Crossreactivity: Human, Mouse, Rat
Format: In PBS, 0.02% sodium azide and 50% glycerol.
Storage: Store at -20C/1 year
Stability: 1 year at -20C
Shipping: Products may be shipped on ice pack or dry ice. |
|
| APPLICATIONS for SHC1 ANTIBODY: |
| WB: Tested |
|
|
| TESTING: (secondary reagents and protocols
) |
IF ( anti-Shc1 )Shc1 antibody image
 IH ( anti-Shc1 )Shc1 antibody image
 WB ( anti-Shc1 )Shc1 antibody image
|
|
|
| SHC1 ANTIBODY TARGET DESCRIPTION: |
Synonym Names for Shc1 antibody: Shc1; SHC; SHCA; SH2 domain protein C1; Src homology 2 domain-containing-transforming protein C1; SHC-transforming protein 1
Function: Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity).
Subunit: Interacts with the Trk receptors in a phosphotyrosine-dependent manner. Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR in vitro via the PID domain. Once activated, binds to GRB2. Interacts with tyrosine-phosphorylated CD3T. Interacts with the N-terminal region of APS. Interacts with phosphorylated LRP1.
Subcellular Location: Cytoplasm. Isoform p46Shc: Mitochondrion matrix. Note=Isoform p46Shc is localized to the mitochondria matrix. Targeting of isoform p46Shc to mitochondria is mediated by its first 32 amino acids, which behave as a bona fide mitochondrial targeting sequence. Isoform p52Shc and isoform p66Shc, that contain the same sequence but more internally located, display a different subcellular localization.
Tissue Specificity: Widely expressed. Expressed in neural stem cells but absent in mature neurons.
Domain: In response to a variety of growth factors, isoform p46Shc and isoform p52Shc bind to phosphorylated Trk receptors through their phosphotyrosine binding (PID) and/or SH2 domains. The PID and SH2 domains bind to specific phosphorylated tyrosine residues in the Asn-Pro-Xaa-Tyr(P) motif of the Trk receptors. Isoform p46Shc and isoform p52Shc are in turn phosphorylated on three tyrosine residues within the extended proline-rich domain. These phosphotyrosines act as docking site for GRB2 and thereby are involved in Ras activation (By similarity).
Ptm: Phosphorylated by activated epidermal growth factor receptor. Isoform p46Shc and isoform p52Shc are phosphorylated on tyrosine residues of the Pro-rich domain. Isoform p66Shc is phosphorylated on Ser-36 upon treatment with insulin, hydrogen peroxide or irradiation with ultraviolet light (By similarity).
Similarity: Contains 1 PID domain.
Similarity: Contains 1 SH2 domain.
Shc1 (Phospho-Tyr427) reacts with human, mouse, rat.
OMIM: 600560; gene. [NCBI / EBI]
Pathways:
KEGG pathway: Chronic myeloid leukemia 05220
KEGG pathway: ErbB signaling pathway 04012
KEGG pathway: Focal adhesion 04510
KEGG pathway: Glioma 05214
KEGG pathway: Insulin signaling pathway 04910
KEGG pathway: Natural killer cell mediated cytotoxicity 04650
Reactome Event:Signaling by insulin receptor 74752
Products similar to Shc1 antibody:
IgG
 Shc1 (Ab-349), IgG
SHC phospho Y239, Y240, IgG
Shc1 (Phospho-Tyr349), IgG
SLI, IgG
Shc1 (Ab-427), IgG
|
|
| PRODUCT INTERACTIONS: |
Shc1 (Phospho-Tyr427) interacts with Amyloid beta A4 protein, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Amyloid beta A4 protein, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with AMYLOID PRECURSOR PROTEIN, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with AMYLOID BETA, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Anti Human Beta Amyloid Protein 1-40 (Anti-betaAP1-40), IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Anti Human Beta Amyloid Protein 17-42 (Anti-betaAP17-42), IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Anti-Human Beta Amyloid Protein Monoclonal Antibody (Anti-Human betaAP), IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Amyloid Precursor Protein [3E9], IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with beta Amyloid [2C8], IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Amyloid Precursor Protein Frameshift Mutant, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Amyloid Precursor Protein, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with beta Amyloid [3G5], IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with beta Amyloid [BAM-10], IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Beta Amyloid N-term. (a.a. 1-8), IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Beta Amyloid N-term. (a.a. 1-8), IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Rabbit anti Beta Amyloid 1-42, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Rabbit anti Beta Amyloid 1-40, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Rabbit A' Amyloid Precursor Pro.(a.a.653-662), IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Rabbit A' Amyloid Precursor Pro.(a.a.737-751), IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Rabbit anti Amyloid, Beta, N-terminal, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Rabbit anti Rat/Mouse Beta Amyloid (3-16), IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Goat A'Amyloid Pre.Pro. Frame (C-term), IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Amyloid Precursor Protein C-term Peptide, Peptide (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Anti-Beta Amyloid, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Amyloid Precursor Protein, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Amyloid Precursor Protein (ready-to-use), IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Amyloid Precursor Protein, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Amyloid Precursor Protein, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with beta Amyloid [DE2B4], IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Beta Amyloid, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Amyloid Precursor Protein, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with PRC1, IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Amyloid beta A4 Variant 40 (A40), IgG (GenWay Catalog #: 18-785-210408).
Shc1 (Phospho-Tyr427) interacts with Amyloid Beta A4 precusor (APP), IgG (GenWay Catalog #: 18-785-210408).
|
|
|
SUGGESTED ANITBODY REAGENTS - GenWay has an extensive selection of reagents useful for antibody applications. Find blocking buffers, stop reagents, chemiluminescent substrates, diluents, Immunohistochemistry reagents & more.
|
|
| BACKGROUND
REFERENCES for SHC1 ANTIBODY: |
| Background references for antibody target are not specific to GenWay products |
[1] Pelicci G., Lanfrancone L., Grignani F., McGlade J., Cavallo F., Forni G., Nicoletti I., Grignani F., Pawson T., Pelicci P.G., et al.
A novel transforming protein (SHC) with an SH2 domain is implicated in mitogenic signal transduction.
[2] Migliaccio E., Mele S., Salcini A.E., Pelicci G., Lai K.M., Superti-Furga G., Pawson T., Di Fiore P.P., Lanfrancone L., Pelicci P.G., et al.
Opposite effects of the p52shc/p46shc and p66shc splicing isoforms on the EGF receptor-MAP kinase-fos signalling pathway.
[3] Harun R.B., Smith K.K., Leek J.P., Markham A.F., Norris A., Morrison J.F.
Characterization of human SHC p66 cDNA and its processed pseudogene mapping to Xq12-q13.1.
[4] Craparo A., O'Neill T.J., Gustafson T.A.
Non-SH2 domains within insulin receptor substrate-1 and SHC mediate their phosphotyrosine-dependent interaction with the NPEY motif of the insulin-like growth factor I receptor.
[5] He W., O'Neill T.J., Gustafson T.A.
Distinct modes of interaction of SHC and insulin receptor substrate-1 with the insulin receptor NPEY region via non-SH2 domains.
[6] Gustafson T.A., He W., Craparo A., Schaub C.D., O'Neill T.J.
Phosphotyrosine-dependent interaction of SHC and insulin receptor substrate 1 with the NPEY motif of the insulin receptor via a novel non-SH2 domain.
[7] van der Geer P., Wiley S., Gish G.D., Pawson T.
The Shc adaptor protein is highly phosphorylated at conserved, twin tyrosine residues (Y239/240) that mediate protein-protein interactions.
[8] Yokouchi M., Suzuki R., Masuhara M., Komiya S., Inoue A., Yoshimura A.
Cloning and characterization of APS, an adaptor molecule containing PH and SH2 domains that is tyrosine phosphorylated upon B-cell receptor stimulation.
[9] Ventura A., Luzi L., Pacini S., Baldari C.T., Pelicci P.-G.
The p66Shc longevity gene is silenced through epigenetic modifications of an alternative promoter.
[10] Salomon A.R., Ficarro S.B., Brill L.M., Brinker A., Phung Q.T., Ericson C., Sauer K., Brock A., Horn D.M., Schultz P.G., et al.
Profiling of tyrosine phosphorylation pathways in human cells using mass spectrometry. |
|
Order Confirmation: Sales order confirmations are sent out upon the receipt of all orders. Please contact GenWay if you do not receive a confirmation within 1 business day of submitting your order.
Precautions: Shc1 antibody is for in vitro research use only. Not for use in diagnostics or therapeutic procedures.
Important Notes: During shipment, small volumes of Shc1 antibody vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. Actual concentration, volume and quantity will be printed on the vial's label. Please refer to the vials label for this information.
Copyright: This GenWay TDS is copyrighted. This datasheet is produced based partially on data from Swiss-Prot/TrEMBL and NCBI. To better serve our clients with everything we know about Shc1 antibody, all related information, articles, resources about Shc1 antibody are being stored on our online database. Let us know if you have questions regarding this product.
Disclaimer: For documents and software available from this server, GenWay neither warrants nor assumes any legal liability or responsibility for the accuracy, completeness or utility of any information, product or process disclosed. |
|
|
|
GenWay is a Protein and Antibody Solutions Provider. Customer Satisfaction is Our Top Priority.
|
|
Thank you for visiting GenWay Biotech's Datasheet. GenWay is the world's leader in chicken, IgY antibody production, producing over 900 quality polyclonal IgY antibodies to date. From our unique recombinant protein production, utilizing a variety of protein expression systems, GenWay's polyclonal IgY antibodies incorporate more epitopes, increasing the success rate for ELISA, western blot, immunohistochemistry (IHC), immunocytochemistry (ICC), and many more immunoassays. |
|
|
|
